Ortho-silicic acid enhances osteogenesis of osteoblasts through the upregulation of miR-130b which directly targets PTEN

You, Yunhao; Ma, Wenzheng; Wang, Fuan; Jiao, Guangjun; Lu, Zhongxin; Zhou, Hongming; Wu, Wenliang; Wang, Hongliang; Chen, Yunzhen

doi:10.1016/j.lfs.2020.118680

Cited by 16 publications

(10 citation statements)

References 62 publications

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“…For reaction bonding, nitrogen at a high temperature is used to heat porous silicon blocks with specific geometries formed from silicon powder. The sintering method refers to the compaction of silicon nitride powder (combined with sintering agent Al 2 O 3 , Y 2 O 3 ), followed by heating in high-temperature N 2 (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30). Based on the sintering method, hot pressing technology can produce more dense silicon nitride than sintering due to the higher given sintering pressure.…”

Section: Molding Process Of Silicon Nitride Implants and Its Impact O...mentioning

confidence: 99%

“…21,22 Studies have demonstrated that silicon has good osteogenic activity, 23,24 and that orthosilicic acid, the only soluble form of silicon in an aqueous solution, can influence a cell's biological behavior intracellularly by modulating various signal transductions, which eventually promotes osteogenesis. 25,26 On the other hand, the N element is present primarily as NH 4 + in a low pH and NH 3 in a high pH. 20 Studies have shown that NH 4 + is involved in the metabolic pathway of glutamine synthetase formation, while glutamine provides the material synthesis raw material for osteogenesis-related cell differentiation and proliferation.…”

Section: Introductionmentioning

confidence: 99%

“…Meanwhile, studies have demonstrated that orthosilicic acid increases the expression of miR-130b, which directly targets PTEN, and that the inhibition of PTEN can significantly up-regulate the downstream factors PI3K and Akt, leading to an increase in mTOR activity. 89,95,96 Enhanced mTOR activity inhibits autophagy, despite the fact that the final product also promotes osteogenic differentiation. However, the regulation of autophagy of osteoblast-related cells by orthosilicic acid released from the surface of silicon nitride may be bidirectional, involving variables such as the concentration of orthosilicic acid.…”

mentioning

confidence: 99%

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Recent advances in the application and biological mechanism of silicon nitride osteogenic properties: a review

Liu,

Wang,

Liu

et al. 2023

Biomater. Sci.

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Section: Molding Process Of Silicon Nitride Implants and Its Impact O...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Recent advances in the application and biological mechanism of silicon nitride osteogenic properties: a review

Liu,

Wang,

Liu

et al. 2023

Biomater. Sci.

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“…The PTEN/PI3K/AKT signaling pathway is widely considered a tumor suppressor pathway; however, in recent years, it has been found that this signaling pathway is also closely related to osteoblast formation ( You et al, 2021 ). There is evidence that PTEN and BMP2 function mutually antagonistically; activated PI3K/AKT is very important for BMP2-induced osteoblast differentiation ( Dong et al, 2021 ).…”

Section: Mechanism Of Mirnas In Bone Regenerationmentioning

confidence: 99%

MicroRNA-loaded biomaterials for osteogenesis

Wang

Cui

et al. 2022

Front. Bioeng. Biotechnol.

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The large incidence of bone defects in clinical practice increases not only the demand for advanced bone transplantation techniques but also the development of bone substitute materials. A variety of emerging bone tissue engineering materials with osteogenic induction ability are promising strategies for the design of bone substitutes. MicroRNAs (miRNAs) are a class of non-coding RNAs that regulate intracellular protein expression by targeting the non-coding region of mRNA3′-UTR to play an important role in osteogenic differentiation. Several miRNA preparations have been used to promote the osteogenic differentiation of stem cells. Therefore, multiple functional bone tissue engineering materials using miRNA as an osteogenic factor have been developed and confirmed to have critical efficacy in promoting bone repair. In this review, osteogenic intracellular signaling pathways mediated by miRNAs are introduced in detail to provide a clear understanding for future clinical treatment. We summarized the biomaterials loaded with exogenous cells engineered by miRNAs and biomaterials directly carrying miRNAs acting on endogenous stem cells and discussed their advantages and disadvantages, providing a feasible method for promoting bone regeneration. Finally, we summarized the current research deficiencies and future research directions of the miRNA-functionalized scaffold. This review provides a summary of a variety of advanced miRNA delivery system design strategies that enhance bone regeneration.

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“…Osteoporosis is a common systemic skeletal disorder characterized by low bone mass and destruction of the bone microstructure, gradually resulting in bone fragility and increased fracture risk [ 1 – 4 ]. During osteoporosis, the adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is enhanced, and osteogenic differentiation is weakened.…”

Section: Introductionmentioning

confidence: 99%

WTAP-mediated m6A modification modulates bone marrow mesenchymal stem cells differentiation potential and osteoporosis

et al. 2023

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An imbalance in the differentiation potential of bone marrow mesenchymal stem cells (BMSCs) is an important pathogenic mechanism underlying osteoporosis (OP). N6-methyladenosine (m6A) is the most common post-transcriptional modification in eukaryotic cells. The role of the Wilms’ tumor 1-associated protein (WTAP), a member of the m6A functional protein family, in regulating BMSCs differentiation remains unknown. We used patient-derived and mouse model-derived samples, qRT-PCR, western blot assays, ALP activity assay, ALP, and Alizarin Red staining to determine the changes in mRNA and protein levels of genes and proteins associated with BMSCs differentiation. Histological analysis and micro-CT were used to evaluate developmental changes in the bone. The results determined that WTAP promoted osteogenic differentiation and inhibited adipogenic differentiation of BMSCs. We used co-immunoprecipitation (co-IP), RNA immunoprecipitation (RIP), methylated RNA immunoprecipitation (MeRIP), RNA pulldown, and dual-luciferase assay to explore the direct mechanism. Mechanistically, the expression of WTAP increased during osteogenic differentiation and significantly promoted pri-miR-181a and pri-miR-181c methylation, which was recognized by YTHDC1, and increased the maturation to miR-181a and miR-181c. MiR-181a and miR-181c inhibited the mRNA expression of SFRP1, promoting the osteogenic differentiation of BMSCs. Our results demonstrated that the WTAP/YTHDC1/miR-181a and miR-181c/SFRP1 axis regulated the differentiation fate of BMSCs, suggesting that it might be a potential therapeutic target for osteoporosis.

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Ortho-silicic acid enhances osteogenesis of osteoblasts through the upregulation of miR-130b which directly targets PTEN

Cited by 16 publications

References 62 publications

Recent advances in the application and biological mechanism of silicon nitride osteogenic properties: a review

Recent advances in the application and biological mechanism of silicon nitride osteogenic properties: a review

MicroRNA-loaded biomaterials for osteogenesis

WTAP-mediated m6A modification modulates bone marrow mesenchymal stem cells differentiation potential and osteoporosis

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