Orthogonal characterization and pharmacokinetic studies of polylactide-polyethyleneglycol polymeric nanoparticles with different physicochemical properties

Processing Parameters and Ion Excipients Affect the Physicochemical Characteristics of the Stereocomplex-Formed Polylactide-b-Polyethylene Glycol Nanoparticles and Their Pharmacokinetics

“…The Animal Ex-perimentation Committee of Kyoto Pharmaceutical University approved all experimental protocols involving animals. PLLA-PEG uni and PDLA-PEG uni were synthesized using previously described procedures [12]. Briefly, mPEG 5 kDa was added to a round-bottom flask and vacuumed at 110 • C. L-or D-lactide was added to the flask for the synthesis.…”

“…Hydrodynamic Diameter (Dh) and Polydispersity Index of SC-PEG NPs (P Dh and PdINP were determined by dynamic light scattering (DLS) using a Nano ZS (Malvern Instruments, Malvern, UK). The measurement methods h described previously [12]. Each sample was diluted 1:50 in 10 mM phosphate bu 7.4) and measured by DLS.…”

“…The concentrations of SC-PEGuni in the NPs were determined as previously d [12]. The prepared NPs were diluted with DMF and analyzed by SEC concentration of SC-PEGuni was calculated using external standard samp concentrations ranging from 2 to 8 mg/mL.…”

“…Conventional Procedure (DLC conv. ) and SPE (DLC SPE ) Concentrations of TAM in NPs were determined using a previously described HPLC procedure [12]. Briefly, prepared NPs were diluted with acetonitrile (MeCN).…”

“…where CTAM load is the TAM concentration in the prepared NPs, Vrec is the volum recovered NP solution, CTAM feed is fed TAM concentration to prepare the NPs, V D h and PdI NP were determined by dynamic light scattering (DLS) using a Zetasizer Nano ZS (Malvern Instruments, Malvern, UK). The measurement methods have been described previously [12]. Each sample was diluted 1:50 in 10 mM phosphate buffer (pH 7.4) and measured by DLS.…”