Orthogonal Reversed-Phase C₁₈ and Pentafluorophenyl HPLC Separation for Phytochemical Profiling of Serrulatanes in Eremophila denticulata

Chemical and Antiplasmodial Investigations on Eremophila-Derived Alkaloids and Semisynthetic Ether Analogues

“…Due to the high degree of ECD data homology between 2 and 1, as well as biosynthetic considerations, the absolute configuration of microthecaline B was assigned as 1R, 3S. 6,16,17 Due to the limited amount of raw root material available for the original isolation work conducted by Kumar et al in 2018 and the low abundance of the novel alkaloid microthecaline A (1), only one analogue was synthesized at that time. 6 For the 10 new semisynthetic analogues described in this paper, we used two ether-forming reactions to make the library.…”

“…The ECD spectrum of 1 showed positive Cotton effects at 208 nm (Δε +7.43), 251 (Δε +2.41), and 287 nm (Δε +1.58), while 2 showed positive Cotton effects at 208 nm (Δε +5.76), 247 (Δε +2.47), and 291 nm (Δε +1.49), respectively (see S40). Due to the high degree of ECD data homology between 2 and 1 , as well as biosynthetic considerations, the absolute configuration of microthecaline B was assigned as 1 R , 3 S . ,, …”