Orthogonal separation protocol for the simultaneous preparation of four medically active compounds from Anemarrhenae Rhizoma by sequential polyamide and macroporous resin adsorbent chromatography

Nian, Sihui; Liu, E‐Hu; Fan, Yong; Alolga, Raphael N.; Li, Huijun; Li, Ping

doi:10.1002/jssc.201600274

Cited by 12 publications

(4 citation statements)

References 22 publications

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“…In addition, the purified mangiferin displayed a convincing IC 50 value of 17.6 µg/mL whereas Vc obtained a value of 11.9 µg/mL. When compared to other investigations, the mangiferin recovered from the current study had 2.18 times more free-radical scavenging activity than the mangiferin purified by a macroporous D101 resin (IC 50 = 38.5 µg/mL) and by solvents with different levels of polarization (IC 50 = 22.2 µg/mL) [ 50 ]. The existence of hydroxyl groups, which serve as metal chelators, and hydrogen donors neutralizers is undoubtedly significant [ 51 ].…”

Section: Resultsmentioning

confidence: 49%

An Insight into In Vitro Antioxidant, Antimicrobial, Cytotoxic, and Apoptosis Induction Potential of Mangiferin, a Bioactive Compound Derived from Mangifera indica

Yehia

Altwaim

2023

Plants

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Due to their low cost, toxicity, and health risks, medicinal plants have come to be seen as useful products and sources of biologically active compounds. Mangifera indica L., a medicinal plant with a long history, has a high bioactive metabolites content. Mangiferin (C19H18O11) is primary isolated from M. indica’s leaves, which has many pharmacological benefits. In this investigation, ultrasonic-assisted extraction with ethanol as the extraction solvent was applied to obtain mangiferin from a local type of M. indica leaves. HPLC was performed after a dichloromethane-ethyl acetate liquid–liquid fractionation method. Further, UV–vis, FTIR, and NMR spectroscopy were utilized to elucidate the structure. Interestingly, purified mangiferin displayed promising antimicrobial efficacy against a diverse variety of fungal and bacterial pathogens with MICs of 1.95–62.5 and 1.95–31.25 µg/mL, respectively. Time–kill patterns also showed that mangiferin had both bactericidal and fungicidal action. Furthermore, it exhibited strong radical dosage-dependent scavenging activity (IC50 = 17.6 μg/mL) compared to vitamin C (Vc, IC50 = 11.9 μg/mL), suggesting it could be developed into a viable antioxidant agent. To our delight, the IC50 values of mangiferin for the MCF-7 and HeLa cell lines were 41.2 and 44.7 μg/mL, respectively, from MTT cell viability testing, and it was less harmful when tested against the noncancerous cell line. Notably, it significantly induced cell apoptosis in MCF-7 cells by 62.2–83.4% using annexin V-FITC/PI labeling. Hence, our findings suggest that mangiferin can be used in the medical industry to create therapeutic interventions and medication delivery systems for society.

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Section: Resultsmentioning

confidence: 49%

An Insight into In Vitro Antioxidant, Antimicrobial, Cytotoxic, and Apoptosis Induction Potential of Mangiferin, a Bioactive Compound Derived from Mangifera indica

Yehia

Altwaim

2023

Plants

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“…The voucher specimen was deposited in School of Pharmacy, Wannan Medical College, Wuhu, Anhui Province. HPLC analysis for AR [26] Qualitative analysis of TS BII and TS AIII was executed according to the Shimadzu LC-20AT…”

Section: Methodsmentioning

confidence: 99%

A Simple and Economic Limewater-based Conversion of Spirostanol Saponins: Using Paridis Rhizoma as a case study

Yu¹,

Bu²,

Shen³

et al. 2020

Preprint

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Background Green and economic conversion of spirostanol saponins in the plant is considered as an attracting area in pharmaceutical applications. We aimed to provide a practical paradigm named limewater-based conversion of spirostanol saponins (LCSS), meanwhile, a widely-used traditional Chinese herbal medicine, Paridis Rhizoma (PR), was selected as a case study. Methods Based on single factor experiments, response surface methodology (RSM) using a Box-Behnken design (BBD) was employed to optimize processing time, limewater concentration and solvent volume to obtain a maximum total saponins yield from PR. H1299, A549 and HeLa cell lines was carried out to check pharmacological effect of Crude Paridis Rhizoma (CPR) and Processed Paridis Rhizoma (PPR), and the technology was reconfirmed by another herbal medicine, Anemarrhenae Rhizoma (AR). Results The optimal conditions were: processing time of 24 h, limewater concentration of 0.12% limewater and solvent volume of 60 mL/30 g. Under these conditions, the contents of polyphyllin VII, polyphyllin II, dioscin, gracillin, and polyphyllin I had 1.131 ± 0.448, 1.835 ± 0.479, 1.430 ± 0.550, 1.761 ± 0.571 and 1.668 ± 0.360 times increasing in four batches of PR, which was responsible for the increasing of total spirostanol saponins (TSS) in PPR. In addition, the extracts of PPR exhibited stronger antitumor potential than that of CPR on H1299, A549 and HeLa cell lines based on MTT test and cell scratch test. The efficiency of proposed LCSS was then reconfirmed by Anemarrhenae Rhizome (AR), indicating its capacity in broader application. Conclusion This study depicted a LCSS strategy that may have great potential in achieving effective and economic improvement of spirostanol saponin accumulation in herbal medicines.

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“…According to previous studies, xanthones, including mangiferin and neomangiferin, and steroidal saponins, including timosaponin BII, timosaponin BIII, and timosaponin AIII, are considered the major bioactive constituents of AR . These compounds show various pharmacological properties, such as antidementia , antidepression , blood glucose lowering , anti‐inflammatory , antiplatelet , and antithrombotic activities .…”

Section: Introductionmentioning

confidence: 99%

A novel ultra high‐performance liquid chromatography‐tandem mass spectrometry method for the simultaneous determination of xanthones and steroidal saponins in crude and salt‐processed Anemarrhenae Rhizoma aqueous extracts

Huang

et al. 2018

J of Separation Science

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We established a rapid and sensitive ultra high-performance liquid chromatography tandem mass spectrometry method for the simultaneous quantification of xanthones and steroidal saponins in rat plasma. Chromatographic separation was achieved on a C column with a mobile phase comprising acetonitrile and 0.1% formic acid. The detection was performed by negative electrospray ionization in multiple reaction monitoring mode. The validated method showed good linearity within the tested range (r > 0.9945). The intra- and interday precision at high, medium, and low concentrations was less than 7.96%. The bias of accuracies ranged from -1.92 to 9.62%. The extraction recoveries of the compounds ranged from 84.78 to 88.69%, and the matrix effects ranged from 96.76 to 108.59%. This method was successfully applied to a pharmacokinetic comparison of crude and salt-processed Anemarrhenae Rhizoma aqueous extracts after oral administration in rats. The maximum plasma concentration and area under concentration-time curve of timosaponin BIII and timosaponin AIII increased significantly (P < 0.05 or 0.01) and those of timosaponin BII decreased significantly (P < 0.05) after processing. These results could contribute to the clinical application of crude and salt-processed Anemarrhenae Rhizoma and reveal the processing mechanism.

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Orthogonal separation protocol for the simultaneous preparation of four medically active compounds from Anemarrhenae Rhizoma by sequential polyamide and macroporous resin adsorbent chromatography

Cited by 12 publications

References 22 publications

An Insight into In Vitro Antioxidant, Antimicrobial, Cytotoxic, and Apoptosis Induction Potential of Mangiferin, a Bioactive Compound Derived from Mangifera indica

An Insight into In Vitro Antioxidant, Antimicrobial, Cytotoxic, and Apoptosis Induction Potential of Mangiferin, a Bioactive Compound Derived from Mangifera indica

A Simple and Economic Limewater-based Conversion of Spirostanol Saponins: Using Paridis Rhizoma as a case study

A novel ultra high‐performance liquid chromatography‐tandem mass spectrometry method for the simultaneous determination of xanthones and steroidal saponins in crude and salt‐processed Anemarrhenae Rhizoma aqueous extracts

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