Orthogonal Test Design for Optimization of the Extraction of Polysaccharides from Inonotus cuticularis and Their Antioxidant Activities

Abstract

Medical fungi polysaccharides belong to a very important species of biological macromolecules, which are the basic substances that effectively maintain and ensure the normal operation of biological life activities. However, research on extraction and biological activity of Inonotus cuticularis polysaccharides has never been reported. In this study, the optimum yield of Inonotus cuticularis polysaccharides was determined by the orthogonal experimental design. The highest yield of 3.10±0.06 % was obtained with extraction temperature of 80 °C, extraction time of 150 min, and water to raw material ratio of 30 mL/g and repeated twice. After deproteinization for 5 times, the protein removal rate reached 70.10±1.75 %, and the content of polysaccharides and protein were 46.64 and 0.42 %. Infrared spectrometer indicated that Inonotus cuticularis polysaccharides are typical β‐pyranose with characteristic peaks of polysaccharides. Subsequently, the activities of scavenging free radicals for the deproteinated polysaccharides were studied. When the concentration of Inonotus cuticularis polysaccharides was 0.3 mg/mL, the scavenging activities of the sample on DPPH., .OH, ABTS.+ and O2.− reached 83.67±0.27, 65.21±4.82, 43.45±1.36 and 80.28±2.30 %, respectively, and the reducing power reached 0.46±0.01. The IC50 values scavenging DPPH., .OH, ABTS.+ and O2.− were 0.139±0.13, 0.162±0.14, 0.317±0.30 and 0.121±0.10 mg/mL, respectively. Results showed that Inonotus cuticularis polysaccharides present potential stronger antioxidant activities, especially .OH scavenging activity and reducing power. Experimental results could provide research basis of Inonotus cuticularis polysaccharides for further exploitation and utilization.

Optimization of Extraction and Purification of Polysaccharides from Veronicastrum axillare, and Evaluation of Their Biological Activities

“…FT‐IR of the VAP ( Figure 5 (a)) showed a strong but wide absorption at 3404 cm −1 , which indicated hydrogen‐bonded hydroxy groups (free hydroxy groups would show absorption at 3560 cm −1 ) [19] . The peak at 2932 cm −1 belonged to methylene C−H anti‐symmetric stretching, which was characteristic to carbohydrates [19] . The peaks at 1743 cm −1 and 1625 cm −1 represented the stretching vibration of the C=O bond [20] .…”

Structure Elucidation of a Novel Polysaccharide Isolated from Euonymus fortunei and Establishing Its Antioxidant and Anticancer Properties

“…However, the single-factor experiments just considered the infuence of each factor on the extraction yield and did not involve the interactions among them, as well as the infuence of each factor on the extraction yield. Terefore, the optimal experimental range of material-liquid ratios, extraction temperature, ultrasonic power, and extraction time was determined by the orthogonal test [44,45]. In the orthogonal test, only nine experiments were conducted for four factors at three levels each, whereas 81 experiments would be necessary for the full-factorial design.…”

Optimization of the Extraction Process and Antioxidant Activity of Polysaccharide Extracted from Centipeda minima

“…The polysaccharide sample was accurately weighed and prepared into a 5 mg/mL solution and then centrifuged at 12000 rpm for 10 min; the supernatant was filtered with a microporous filter membrane, and the transferred into a 2 mL injection vial. The molecular weight of the Centipeda minima polysaccharide was determined using the HPGPC method [36–37] . Dextran with known molecular weight was used as the standard to construct a standard curve.…”