Orthogonal test design for optimization of lipid accumulation and lipid property in Nannochloropsis oculata for biodiesel production

Wei, Likun; Huang, Xuxiong; Huang, Zhenzheng; Zhou, Zhigang

doi:10.1016/j.biortech.2013.08.079

Cited by 30 publications

(21 citation statements)

References 28 publications

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“…oculata with a high TL content [14]. However, little information is available on the regulatory processes of multi-factor collaborative stresses (MFCS) on the oil-yielding performances of N .…”

Section: Introductionmentioning

confidence: 99%

Long-duration effect of multi-factor stresses on the cellular biochemistry, oil-yielding performance and morphology of Nannochloropsis oculata

Wei

Huang

2017

PLoS ONE

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Microalga Nannochloropsis oculata is a promising alternative feedstock for biodiesel. Elevating its oil-yielding capacity is conducive to cost-saving biodiesel production. However, the regulatory processes of multi-factor collaborative stresses (MFCS) on the oil-yielding performance of N. oculata are unclear. The duration effects of MFCS (high irradiation, nitrogen deficiency and elevated iron supplementation) on N. oculata were investigated in an 18-d batch culture. Despite the reduction in cell division, the biomass concentration increased, resulting from the large accumulation of the carbon/energy-reservoir. However, different storage forms were found in different cellular storage compounds, and both the protein content and pigment composition swiftly and drastically changed. The analysis of four biodiesel properties using pertinent empirical equations indicated their progressive effective improvement in lipid classes and fatty acid composition. The variation curve of neutral lipid productivity was monitored with fluorescent Nile red and was closely correlated to the results from conventional methods. In addition, a series of changes in the organelles (e.g., chloroplast, lipid body and vacuole) and cell shape, dependent on the stress duration, were observed by TEM and LSCM. These changes presumably played an important role in the acclimation of N. oculata to MFCS and accordingly improved its oil-yielding performance.

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Section: Introductionmentioning

confidence: 99%

Long-duration effect of multi-factor stresses on the cellular biochemistry, oil-yielding performance and morphology of Nannochloropsis oculata

Wei

Huang

2017

PLoS ONE

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“…The orthogonal test is an effective measurement to assay the comprehensive effect of multiple factors (Wei et al, 2013). An L 27 (3 13 ) orthogonal test probing the frequency sweeping rate, acceleration amplitude, laying style, and their 2-way interactions was designed according to the results of the single-factor experiments.…”

Section: Methodsmentioning

confidence: 99%

Orthogonal test design to optimize the acoustic vibration method for pear texture measurement

Wen

Cui

2015

Postharvest Biology and Technology

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“…It has high lipid content, being a promising species for the production of biodiesel; it is also used as food in aquaculture, especially for the larval stage of fish and crustaceans due to its easy and rapid cultivation and small size compatible with the feeding mechanism of larvae (WEI et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Effect of nitrate depletion on lipid accumulation by the marine microalga Nannochloropsis oculata

Silva¹,

Araújo²,

Coelho³

et al. 2015

Bol. Inst. Pesca

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Nannochloropsis oculata is a marine algae with high lipid content, being a promising species for biofuel production. It is also used as food in aquaculture for larval fish and crustaceans due to its high nutritional value and compatible size with the larvae feeding. This study evaluated the lipid content in the biomass of N. oculata under nitrate depletion. The cultivation was carried out in 8 L bottles with three replications, at salinity 27, illuminance of 60 μE cm -2 s -1 and temperature 28 °C. The levels of sodium nitrate used were 15; 30; 45; 60 and 75 mg L -1 , while maintaining constant the levels of all other nutrients of the Guillard f/2 medium. The growth curve was monitored every two days by spectrophotometry at 680 nm (DO680nm) and cell counting in a Neubauer chamber. For separation of the culture medium from the cells, we used chemical flocculation method, adding 2N NaOH. The higher lipid content in the biomass was obtained at the lowest level of nitrate in the culture medium. Keywords

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Orthogonal test design for optimization of lipid accumulation and lipid property in Nannochloropsis oculata for biodiesel production

Cited by 30 publications

References 28 publications

Long-duration effect of multi-factor stresses on the cellular biochemistry, oil-yielding performance and morphology of Nannochloropsis oculata

Long-duration effect of multi-factor stresses on the cellular biochemistry, oil-yielding performance and morphology of Nannochloropsis oculata

Orthogonal test design to optimize the acoustic vibration method for pear texture measurement

Effect of nitrate depletion on lipid accumulation by the marine microalga Nannochloropsis oculata

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