Orthopoxvirus DNA: Strain differentiation by electrophoresis of restriction endonuclease fragmented virion DNA

Esposito, Joseph J.; Obijeski, John F.; Nakano, James H.

doi:10.1016/0042-6822(78)90039-9

Cited by 90 publications

(34 citation statements)

References 41 publications

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“…CPDV was purified from scabs obtained from an experimentally inoculated sheep. Scabs from the groin region were collected 10-14 days after inoculation and processed [10,11]. Approximately 3-4 g of tissue was mixed with carborundum (Turnpike Road, Cressex Industrial State, High Wycombe HP12 3NR, U.K.) in 15 ml TE buffer solution (10 mM Tris-HCl, pH 7-5 and 01 mM EDTA) and ground with a pestle and mortar.…”

Section: Purification Of Virusesmentioning

confidence: 99%

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Western blot analysis of virus-specific antibody responses for capripox and contagious pustular dermatitis viral infections in sheep

1994

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SUMMARYThis paper reports the development and evaluation of serological tests for the differentiation of antibodies in animals infected with capripox and parapox viruses. Agar-gel immunodiffusion tests using sera from sheep with naturallyacquired infections and from sheep experimentally inoculated with orf or capripox viruses showed cross reactions. Virus-specific antibody responses to structural proteins of the viruses were analysed by Western-blot analysis. This analysis readily differentiated the infections as either capripox or contagious pustular dermatitis. The antibody responses to the 32 kDa and 26 kDa proteins of capripoxvirus provided a firm basis for differention.

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Section: Purification Of Virusesmentioning

confidence: 99%

“…Sheep inoculated with CPDV were given additional intramuscular inoculations (2-5 ml of 20 % autogenous CPD-scab) 16 and 18 weeks after the first inoculation. The serum obtained 10 …”

Section: Hyperimmune Antiseramentioning

confidence: 99%

Western blot analysis of virus-specific antibody responses for capripox and contagious pustular dermatitis viral infections in sheep

1994

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“…DNA HindlII fragment analysis of poxviruses has provided a useful approach to taxonomic and epidemiological studies (Esposito et al, 1978;Esposito & Knight, 1985;Rosen et al, 1987;Gershon & Black, 1988). The ERPV P-4 HindlII electropherogram was similar to, but different from, that of CECT, which further implies that ERPV might be either a new poxvirus or a subspecies of ectromelia virus.…”

Section: Discussionmentioning

confidence: 97%

Further characterization of the biological and pathogenic properties of erythromelalgia-related poxviruses

Zheng

Specter

Jiang-Hong³

et al. 1992

Journal of General Virology

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Six isolates of erythromelalgia-related poxvirus (ERPV) were characterized with respect to host range, c.p.e, and inclusions, pock formation on chorioallantoic membrane (CAM), morphogenesis, serological reactivity, pathogenesis in animals and DNA restriction fragment profile. The results suggest that ERPV is either a new member of the Orthopoxvirus genus or a subspecies of ectromelia virus. Evidence is provided that (i) ERPV has a wide host range in vitro in which characteristic viral c.p.e, and inclusion bodies are induced; (ii) ERPV, unlike ectromelia virus, causes the formation of tiny greyish-white pocks on CAM both at 34 °C and 39 *C; (iii) eosinophilic A-type inclusions of ERPV do not contain viral particles; (iv) ERPV isolates are neutralized by both rabbit anti-vaccinia virus and mouse anti-ectromelia virus sera, but not vice versa; (v) young rabbits are not susceptible to ERPV by skin and/or corneal scratch infection even though ERPV is lethal for mice by intraperitoneal inoculation; (vi) the HindlII and Sail fragment profiles ofERPV P-4 DNA are similar to, but obviously different from, those of Chinese ectromelia virus. These biological and pathogenic characteristics of ERPV are distinguishable from those of other members of the genus Orthopoxvirus currently described in the literature.

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“…As a source for the target B9R gene (Fig. I) [1,2] DNA fragments of the highly virulent India-1967 (IND) strain of VAR (variola major) [4] and low virulent Garcia-1966 (GAR) strain of VAR (variola minor) [5] which were cloned in bacterial plasmids [6] were used.…”

Section: Introductionmentioning

confidence: 99%

Comparative studies of gamma‐interferon receptor‐like proteins of variola major and variola minor viruses

et al. 1996

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Orthopoxvirus DNA: Strain differentiation by electrophoresis of restriction endonuclease fragmented virion DNA

Cited by 90 publications

References 41 publications

Western blot analysis of virus-specific antibody responses for capripox and contagious pustular dermatitis viral infections in sheep

Western blot analysis of virus-specific antibody responses for capripox and contagious pustular dermatitis viral infections in sheep

Further characterization of the biological and pathogenic properties of erythromelalgia-related poxviruses

Comparative studies of gamma‐interferon receptor‐like proteins of variola major and variola minor viruses

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