Orthosomella lipae sp. n. (Microsporidia) a parasite of the weevil, Liophloeus lentus Germar, 1824 (Coleoptera: Curculionidae)

Ovcharenko, Mykola; Świątek, Piotr; Ironside, Joseph E.; Skalski, Tomasz

doi:10.1016/j.jip.2012.10.007

Cited by 11 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thelonia contejeani and Thelonia parastaci were used as outgroups in the analysis. (Becnel et al, 2002;Ovcharenko et al, 2013). These previous molecular phylogenetic analyses have been confirmed by our results.…”

Section: Discussionsupporting

confidence: 91%

Morphological, ultrastructural, and molecular identification of a new microsporidian pathogen isolated from Crepidodera aurata (Coleoptera, Chrysomelidae)

Yaman¹,

Algı²,

Radek³

2019

Turk J Zool

View full text Add to dashboard Cite

A new microsporidian pathogen isolated from Crepidodera aurata was identified based on morphological and ultrastructural characteristics, coupled with a molecular phylogenetic analysis. The spores of the microsporidian pathogen were slightly curved in shape, and measured 2.44-3.55 µm in length and 1.25-1.55 µm in width (n = 50). Its ultrastructure is characteristic of monokaryotic groups. All lifecycle stages of the pathogen, including meronts, sporonts, sporoblasts, and mature spores, are monokaryotic. The spore has 6-8 windings of the polar filament. Morphological and ultrastructural characteristics of the lifecycle stages place it within the family Unikaryonidae. However, the phylogenetic tree constructed on the 16S rRNA gene sequence analysis indicates that the pathogen is closely related to the Nosema/Vairimorpha clade of microsporidia. Therefore, we have classified the microsporidian of C. aurata in the tentative group Microsporidium in order to avoid creating an unnecessary or incorrect new genus/species.

show abstract

Section: Discussionsupporting

confidence: 91%

Morphological, ultrastructural, and molecular identification of a new microsporidian pathogen isolated from Crepidodera aurata (Coleoptera, Chrysomelidae)

Yaman¹,

Algı²,

Radek³

2019

Turk J Zool

View full text Add to dashboard Cite

show abstract

“…They are particularly well studied in arthropods and fish (Keeling and Fast 2002;Smith 2009). Notably, microsporidia have been documented in the most diverse insect orders, including Coleoptera (e.g., Yaman et al 2010;Kyei-Poku et al 2011;Ovcharenko et al 2013), Lepidoptera (e.g., Solter et al 2000), and Hymenoptera and Diptera (see references below). Some microsporidian species can infect many distantly related hosts, but specificity to a single host or a group of closely related hosts may predominate (Baker et al 1998;Keeling and Fast 2002;Smith 2009;Vávra and Lukeš 2013).…”

Section: Animal-associated Fungi Microsporidiamentioning

confidence: 99%

Inordinate Fondness Multiplied and Redistributed: the Number of Species on Earth and the New Pie of Life

Larsen

Miller

Rhodes

et al. 2017

The Quarterly Review of Biology

286

221

View full text Add to dashboard Cite

The number of species on Earth is one of the most fundamental numbers in science, but one that remains highly uncertain. Clearly, more species exist than the present number of formally described species (approximately 1.5 million), but projected species numbers differ dramatically among studies. Recent estimates range from about 2 million species to approximately 1 trillion, but most project around 11 million species or fewer. Numerous studies have focused on insects as a major component of overall richness, and many have excluded other groups, especially non-eukaryotes. Here, we re-estimate global biodiversity. We also estimate the relative richness of the major clades of living organisms, summarized as a "Pie of Life." Unlike many previous estimates, we incorporate morphologically cryptic arthropod species from molecular-based species delimitation. We also include numerous groups of organisms that have not been simultaneously included in previous estimates, especially those often associated with particular insect host species (including mites, nematodes, apicomplexan protists, microsporidian fungi, and bacteria). Our estimates suggest that there are likely to be at least 1 to 6 billion species on Earth. Furthermore, in contrast to previous estimates, the new Pie of Life is dominated by bacteria (approximately 70-90% of species) and insects are only one of many hyperdiverse groups. 230 Volume 92 THE QUARTERLY REVIEW OF BIOLOGY

show abstract

“…The present work is the rst to describe the occurrence of microsporidiosis in an insect colony from the second generation and in eld populations of eucalyptus snout beetles collected in commercial Eucalyptus plantations, with the disease being con rmed in two species present in Brazil, G. platensis and G. pulverulentus (Coleoptera: Curculionidae). Microsporidian infections have been reported in other Curculionidae 17,18 as well as other coleopterans 19,20,21,22, which demonstrate that this fungal pathogen is more common than previously thought infecting this diverse insect order. Adult beetles collected across the three Brazilian tested positive for the presence of this pathogen.…”

Section: Discussionmentioning

confidence: 92%

First Record of A New Microsporidium Pathogenic to the Eucalyptus Snout Beetle in Brazil

Jordan¹,

Carvalho²,

Mascarin³

et al. 2021

Preprint

View full text Add to dashboard Cite

Microsporidia are naturally occurring fungal-related parasites that can infect nearly all animal hosts, but their biocontrol potential of insect pests is routinely overlooked in agriculture and forestry. This research brings the first report describing the natural occurrence of a microsporidium causing disease in field-collected populations of the invasive eucalyptus snout beetle, Gonipterus platensis (Coleoptera: Curculionidae), a major destructive pest of eucalyptus plantations in Brazil. Adult beetles were collected during field surveys in commercial eucalyptus plantations in southern Brazil to be examined and dissected with typical symptoms to verify presence of microsporidian spores in haemolymph. From 14 plantations in different sites, the natural infection occurrence in these populations ranged from 0 to 65%, while a lab colony exhibited an infection incidence of 70%. Spore density in haemolymph of symptomatic insects averaged 2.1(±0.4) × 107 spores/mL. Symptoms in infected adults were identified by abnormal abdomen with malformation of the second pair of wings, impairing their flight activity. Electron transmission microscopy of the pathogen showed morphological features similar to species belonging to the genus Nosema or Vairimorpha. Phylogenetic analysis of the full-length small subunit ribosomal RNA gene suggests this pathogen’s placement in the genus Vairimorpha, but with a sequence identity of ~94% with the nearest neighbours. The low level of sequence identity suggests this pathogen may represent a novel taxon in the genus and further requires whole genome sequencing for definitive taxonomic resolution. These findings provide insights on the natural occurrence of this novel pathogen for potential use in biocontrol of this invasive pest in Eucalyptus plantations in Brazil, and prompts the design of conservative or augmentative strategies for the establishment of this microsporidium in field populations for sustainable management of eucalyptus snout beetles.

show abstract

Orthosomella lipae sp. n. (Microsporidia) a parasite of the weevil, Liophloeus lentus Germar, 1824 (Coleoptera: Curculionidae)

Cited by 11 publications

References 21 publications

Morphological, ultrastructural, and molecular identification of a new microsporidian pathogen isolated from Crepidodera aurata (Coleoptera, Chrysomelidae)

Morphological, ultrastructural, and molecular identification of a new microsporidian pathogen isolated from Crepidodera aurata (Coleoptera, Chrysomelidae)

Inordinate Fondness Multiplied and Redistributed: the Number of Species on Earth and the New Pie of Life

First Record of A New Microsporidium Pathogenic to the Eucalyptus Snout Beetle in Brazil

Contact Info

Product

Resources

About