Orthotopic corneal transplantation in the mouse — a new surgical technique with minimal endothelial cell loss

Zhang, Er-Ping; Schründer, Stephan; Hoffmann, Friedrich

doi:10.1007/bf00292359

Cited by 44 publications

(38 citation statements)

References 16 publications

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“…Experimental corneal grafts were performed as previously described [20]. Briefly, before all surgical procedures mice were deeply anesthetized by intraperitoneal injection of 3 mg ketamine (Ketavet, Pharmacia GmbH, Germany) and 0.0075 mg xylazine (Rompun 2%, Bayer Vital GmbH, Germany).…”

Section: Orthotopic Corneal Transplantationmentioning

confidence: 99%

Influence of combined treatment of low dose rapamycin and cyclosporin A on corneal allograft survival

Stanojlović

Schlickeiser

Appelt

et al. 2010

Graefes Arch Clin Exp Ophthalmol

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Section: Orthotopic Corneal Transplantationmentioning

confidence: 99%

Influence of combined treatment of low dose rapamycin and cyclosporin A on corneal allograft survival

Stanojlović

Schlickeiser

Appelt

et al. 2010

Graefes Arch Clin Exp Ophthalmol

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“…Experimental keratoplasty in mice was performed as previously described (16). Briefly, mice were anesthetized by i.p.…”

Section: Orthotopic Corneal Transplantationmentioning

confidence: 99%

Control of TNF-Induced Dendritic Cell Maturation by Hybrid-Type N-Glycans

Schlickeiser

Stanojlović²,

Appelt

et al. 2011

The Journal of Immunology

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The activity of α-1,2-mannosidase I is required for the conversion of high-mannose to hybrid-type (ConA reactive) and complex-type N-glycans (Phaseolus vulgaris-leukoagglutinin [PHA-L] reactive) during posttranslational protein N-glycosylation. We recently demonstrated that α-1,2-mannosidase I mRNA decreases in graft-infiltrating CD11c+ dendritic cells (DCs) prior to allograft rejection. Although highly expressed in immature DCs, little is known about its role in DC functions. In this study, analysis of surface complex-type N-glycan expression by lectin staining revealed the existence of PHA-Llow and PHA-Lhigh subpopulations in murine splenic conventional DCs, as well as in bone marrow-derived DC (BMDCs), whereas plasmacytoid DCs are nearly exclusively PHA-Lhigh. Interestingly, all PHA-Lhigh DCs displayed a strongly reduced responsiveness to TNF-α–induced p38-MAPK activation compared with PHA-Llow DCs, indicating differences in PHA-L–binding capacities between DCs with different inflammatory properties. However, p38 phosphorylation levels were increased in BMDCs overexpressing α-1,2-mannosidase I mRNA. Moreover, hybrid-type, but not complex-type, N-glycans are required for TNF-α–induced p38-MAPK activation and subsequent phenotypic maturation of BMDCs (MHC-II, CD86, CCR7 upregulation). α-1,2-mannosidase I inhibitor-treated DCs displayed diminished transendothelial migration in response to CCL19, homing to regional lymph nodes, and priming of IFN-γ–producing T cells in vivo. In contrast, the activity of α-1,2-mannosidase I is dispensable for LPS-induced signaling, as well as the DCs’ general capability for phenotypic and functional maturation. Systemic application of an α-1,2-mannosidase I inhibitor was able to significantly prolong allograft survival in a murine high-responder corneal transplantation model, further highlighting the importance of N-glycan processing by α-1,2-mannosidase I for alloantigen presentation and T cell priming.

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“…Procedure for transplantation and post-operative assessment of rejection was as described [47,48], with the assessment being done by a masked observer. All transplants were unilateral unless otherwise specified, on the right eye.…”

Section: Corneal Transplantationmentioning

confidence: 99%

Function of indoleamine 2,3‐dioxygenase in corneal allograft rejection and prolongation of allograft survival by over‐expression

et al. 2006

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Indoleamine 2,3-dioxygenase (IDO) suppresses T cell responses by its action in catabolising tryptophan. It is important in maintenance of immune privilege in the placenta. We investigated the activity of IDO in the cornea, following corneal transplantation and the effect of IDO over-expression in donor corneal endothelium on the survival of corneal allografts. IDO expression was analysed and functional activity was quantified in normal murine cornea and in corneas following transplantation as allografts. Low levels of IDO, at both mRNA and protein levels, was detected in the normal cornea, up-regulated by IFN-c and TNF. Expression of IDO in cornea was significantly increased following corneal transplantation. However, inhibition of IDO activity in vivo had no effect on graft survival. Following IDO cDNA transfer, murine corneal endothelial cells expressed functional IDO, which was effective at inhibiting allogeneic T cell proliferation. Over-expression of IDO in donor corneal allografts resulted in prolonged graft survival. While, on one hand, our data indicate that IDO may augment corneal immune privilege, up-regulated IDO activity following cytokine stimulation may serve to inhibit inflammatory cellular responses. While increasing IDO mRNA expression was found in allogeneic corneas at rejection, over-expression in donor cornea was found to significantly extend survival of allografts.

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Orthotopic corneal transplantation in the mouse — a new surgical technique with minimal endothelial cell loss

Cited by 44 publications

References 16 publications

Influence of combined treatment of low dose rapamycin and cyclosporin A on corneal allograft survival

Influence of combined treatment of low dose rapamycin and cyclosporin A on corneal allograft survival

Control of TNF-Induced Dendritic Cell Maturation by Hybrid-Type N-Glycans

Function of indoleamine 2,3‐dioxygenase in corneal allograft rejection and prolongation of allograft survival by over‐expression

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