Orthotopic Osteogenecity Enhanced by a Porous Gelatin Sponge in a Critical‐Sized Rat Calvaria Defect

Kanda, Naoki; Anada, Takahisa; Handa, Tomoyuki; Kobayashi, Kuriko; Ezoe, Yushi; Takahashi, Tsuneo; Сузукі, Осаму

doi:10.1002/mabi.201500191

Cited by 22 publications

(23 citation statements)

References 48 publications

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“…8). 53 It is probable that the ability of gelatin itself 53 should contribute enhancing bone formation by OCP/Gel more than that by OCP granules alone (Fig. 9).…”

Section: Discussionmentioning

confidence: 99%

Osteogenic cellular activity around onlaid octacalcium phosphate‐gelatin composite onto rat calvaria

Iwama

Anada

Tsuchiya

et al. 2018

J Biomedical Materials Res

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The activity and the distribution of osteogenic cells around octacalcium phosphate (OCP) granules combined with gelatin matrix (OCP/Gel) onlaid on rat calvaria were analyzed histomorphometrically and immunohistochemically during vertical bone augmentation under mechanical or nonmechanical environment until 8 weeks. OCP/Gel disk was placed in subperiosteal pocket on the calvaria with or without polytetrafluoroethylene (PTFE) support. The latter is a nonmechanical stress model to alleviate the mechanical stress from the subcutaneous tissues. Onlay grafts of gelatin (Gel) sponge disk and OCP granules were also carried out for the comparison purpose. When bone augmentation was evaluated in first area from bone surface (area until 150 μm high from bone surface) and second area above the newly formed bone (area until 150 µm high from the first area), bone formation was enhanced most in first area followed by second area of OCP/Gel with PTFE. The appearance of tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like cells was suppressed more in the newly formed tissue with PTFE than those without PTFE with an emphasis of the presence of gelatin. Although Runx2 positive-cells were accumulated more in both OCP/Gel with and without PTFE, osteocalcin-positive cells were abundant in OCP/Gel with PTFE than that without PTFE, suggesting that nonmechanical stress condition is more suitable for osteoblast differentiation. The appearance of receptor activator of nuclear factor (NF)-kappaB ligand (RANKL)-positive cells was restrained in OCP/Gel and Gel with PTFE while osteoprotegerin-positive cells were most accumulated in OCP/Gel without PTFE. The results suggest that OCP/Gel composite under the mechanical stress-alleviated condition can enhance bone augmentation through balanced osteoblastic and osteoclastic cellular activities. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1322-1333, 2018.

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“…8). 53 It is probable that the ability of gelatin itself 53 should contribute enhancing bone formation by OCP/Gel more than that by OCP granules alone (Fig. 9).…”

Section: Discussionmentioning

confidence: 99%

Osteogenic cellular activity around onlaid octacalcium phosphate‐gelatin composite onto rat calvaria

Iwama

Anada

Tsuchiya

et al. 2018

J Biomedical Materials Res

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“…It is known that gelatin molecule has a cellular binding motif and the reconstituted gelatin has been used as a biomedical material as well as collagen . A recent study indicated that Gelatin prepared in specific condition induces orthotopic bone formation in critical‐sized defect of rat calvaria . However, it is recognized that Gelatin sponge does not positively induce the bone formation usually .…”

Section: Discussionmentioning

confidence: 99%

“…38 A recent study indicated that Gelatin prepared in specific condition induces orthotopic bone formation in critical-sized defect of rat calvaria. 28 However, it is recognized that Gelatin sponge does not positively induce the bone formation usually. 39 This is because active molecules, such as bone morphogenetic proteins, are included in Gelatin in tissue regeneration.…”

Section: Discussionmentioning

confidence: 99%

Effect of resorption rate and osteoconductivity of biodegradable calcium phosphate materials on the acquisition of natural bone strength in the repaired bone

Chiba

Anada

Suzuki

et al. 2016

J Biomedical Materials Res

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The purpose of this study was to compare the biodegradation rate and quality of regenerated bone among four materials. A short time period of 8 weeks was chosen to examine early bone healing. The rod-shaped implants of commercially available two β-tricalcium phosphate (β-TCP) ceramics with porosity 60% and 71-80%, respectively, laboratory prepared octacalcium phosphate/gelatin composite (OCP/Gel), which has been proven to have a highly osteoconductive and biodegradable property in rat calvarial defect, and gelatin sponge (Gelatin) were implanted in rabbit tibia defect of 6 mm diameter and 7 mm depth for 2, 4 and 8 weeks. Analyses by μCT, histomorphometry and push-in test were carried out to evaluate the extent of the tissue regeneration and the material biodegradation in the long bone. OCP/Gel and Gelatin were completely resorbed but only OCP/Gel induced cortical bone bridge until 8 weeks that has strength compatible to that of the natural bone. β-TCP (71%-80%) and β-TCP (60%) were not completely resorbed and never induced the amount of new bone formation beyond that by OCP/Gel. The results indicate that the new bone having enough strength could be regenerated if the material shows not only higher biodegradation rate but also higher osteoconductivity. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2833-2842, 2016.

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“…Gelatin is a partial hydrolysis product of native collagen and characterized by non-toxicity, non-carcinogenicity, biocompatibility, and biodegradability 1 – 3 ; gelatin is widely used in the pharmaceutical and medical fields, such as in wound dressing materials 4 , 5 , tissue engineering scaffolds 6 – 8 , and drug delivery carriers 9 , 10 . Gelatin can be prepared in a spongy form to be suitable for tissue engineering applications.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the characteristics and biocompatibility of gelatin sponge scaffolds prepared by various crosslinking methods

Yang

Xiao

Long³

et al. 2018

Sci Rep

192

156

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This comparative study aims to identify a biocompatible and effective crosslinker for preparing gelatin sponges. Glutaraldehyde (GTA), genipin (GP), 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide (EDC), and microbial transglutaminase (mTG) were used as crosslinking agents. The physical properties of the prepared samples were characterized, and material degradation was studied in vitro with various proteases and in vivo through subcutaneous implantation of the sponges in rats. Adipose-derived stromal stem cells (ADSCs) were cultured and inoculated onto the scaffolds to compare the cellular biocompatibility of the sponges. Cellular seeding efficiency and digestion time of the sponges were also evaluated. Cellular viability and proliferation in scaffolds were analyzed by fluorescence staining and MTT assay. All the samples exhibited high porosity, good swelling ratio, and hydrolysis properties; however, material strength, hydrolysis, and enzymolytic properties varied among the samples. GTA–sponge and GP–sponge possessed high compressive moduli, and EDC–sponge exhibited fast degradation performance. GTA and GP sponge implants exerted strong in vivo rejections, and the former showed poor cell growth. mTG–sponge exhibited the optimal comprehensive performance, with good porosity, compressive modulus, anti-degradation ability, and good biocompatibility. Hence, mTG–sponge can be used as a scaffold material for tissue engineering applications.

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Orthotopic Osteogenecity Enhanced by a Porous Gelatin Sponge in a Critical‐Sized Rat Calvaria Defect

Cited by 22 publications

References 48 publications

Osteogenic cellular activity around onlaid octacalcium phosphate‐gelatin composite onto rat calvaria

Osteogenic cellular activity around onlaid octacalcium phosphate‐gelatin composite onto rat calvaria

Effect of resorption rate and osteoconductivity of biodegradable calcium phosphate materials on the acquisition of natural bone strength in the repaired bone

Assessment of the characteristics and biocompatibility of gelatin sponge scaffolds prepared by various crosslinking methods

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