Orthotopic transplantation of LH receptor knockout and wild-type ovaries

Chudgar, Daksha; Lei, Zhenmin; Rao, Ch.V.

doi:10.1016/j.lfs.2005.03.024

Cited by 11 publications

(8 citation statements)

References 29 publications

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“…The increased Lhcgr expression at the time of implantation provides further support for a possible role of LHCGR signalling at the maternal-fetal interface during embryo implantation in mice. Our results also agree with those from a study by Chudgar et al (2005), who found that LuRKO mice were not able to become pregnant after hormonal substitution or after orthotopic transplantation of wild-type ovaries. The contribution of uterine LHCGR signalling to the infertility of LuRKO mice was demonstrated by one group (Lin et al 2005); however, conflicting results were published by Pakarainen et al (2005), who were unable to show that orthotopic transplantation of wild-type ovaries into an LuRKO mouse resulted in impaired implantation.…”

Section: Discussionsupporting

confidence: 93%

Evidence for cross-talk between the LH receptor and LH during implantation in mice

Gridelet

Tsampalas

Berndt

et al. 2013

Reprod. Fertil. Dev.

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Abstract. The present study investigated the first interaction that occurs between the blastocyst and endometrium during implantation. Given the ethical objections to studying implantation in humans, a mouse model was used to study the dialogue between luteinising hormone (LH) and luteinising hormone receptor (LHCGR). Several studies performed on LHCGR-knockout mice have generated controversy regarding the importance of the dialogue between LH and LHCGR during implantation. There has been no demonstration of a bioactive LH-like signal produced by the murine blastocyst. The first aim of the present study was to examine and quantify, using radioimmunoassay, the generation of a bioactive LH signal by the murine blastocyst. We went on to examine and quantify endometrial Lhcgr expression to validate the mouse model. Expression of LHCGR in mouse uteri was demonstrated using immunohistochemistry and western blot analysis. To quantify the expression of Lh in the mouse blastocyst and Lhcgr in the endometrium, reverse transcription-polymerase chain reaction (RT-PCR) and real-time quantitative (q) RT-PCR were performed. The results demonstrate that Lhcgr expression in BALB/c mouse endometrial epithelium is increased at the time of implantation and indicate that LHCGR may contribute to the implantation process. In support of this hypothesis, we identified a bioactive LH signal at the time of murine blastocyst implantation.

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Section: Discussionsupporting

confidence: 93%

Evidence for cross-talk between the LH receptor and LH during implantation in mice

Gridelet

Tsampalas

Berndt

et al. 2013

Reprod. Fertil. Dev.

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show abstract

“…Especially if the results are negative, in the absence of positive control, it remains a possibility that the exogenous hormone treatment has not been able to simulate completely that caused by endogenous hormones. The same auhors were also unable to achieve pregnancies following orthotopic transplantations of WT ovarian tissue in their LHR KO mice (Chudgar et al, 2005), which negative finding also remains inconclusive.…”

Section: Orthotopic Ovarian Transplantation In Lurko Micementioning

confidence: 97%

Extragonadal LH/hCG action—Not yet time to rewrite textbooks

Pakarainen¹,

Ahtiainen²,

Zhang³

et al. 2007

Molecular and Cellular Endocrinology

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Please cite this article as: Pakarainen, T., Ahtiainen, P., Zhang, F.-P., Rulli, S., Poutanen, M., Huhtaniemi, I., Extragonadal LH/hCG action -not yet time to rewrite textbooks, Molecular and Cellular Endocrinology (2007), doi:10.1016/j.mce.2006 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.A c c e p t e d M a n u s c r i p t A c c e p t e d M a n u s c r i p t AbstractGonadotropins are indispensable in both sexes in the regulation of gonadal sex steroid production and gametogenesis. In addition to their well established classical actions, numerous recent publications have indicated the presence and function of luteinizing hormone/chorionic gonadotropin receptors (LH/hCG-R) in a variety of extragonadal tissues. However, the physiological significance of such effects has remained unclear. We have generated two genetically modified mouse models, one with excessive production of hCG and the other with targeted disruption of LH/hCG-R gene, and used them to address the functions of LH and hCG.Numerous gonadal and extragonadal phenotypes were found in the models with the two extremes of LH/hCG action. However, when the extragonadal effects were scrutinized in greater detail, they all appeared to arise through modification of gonadal function, either through enhanced or inhibited response to LH/hCG stimulation. Hence, further evidence is needed before the extragonadal LH/hCG-R expression can be considered functionally significant.

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“…Leptin mRNA and protein were detected in human granulosa and theca cells, follicular fluid, oocytes, and corpus luteum (44)(45)(46)(47) as well as in these tissues in mice (41,48). The effects of leptin were mediated through the activation of the receptor of the long (Ob-Rb) form, and the Ob-Rb has been found in human and mouse granulose and theca cells and stromal tissues (29)(30)(31).…”

Section: Discussionmentioning

confidence: 99%

“…At 12 weeks of age, db/db and WT mice received reciprocal ovary transplantation as shown in Figure 1 (41). Experiment 1: To determine whether defective leptin signaling within local ovary contributes to the impairment of reproduction and metabolism in db/db mice, we performed reciprocal ovary transplantation between db/db and WT mice.…”

Section: Ovary Transplantationmentioning

confidence: 99%

The impairment of reproduction in db/db mice is not mediated by intraovarian defective leptin signaling

Zhang

et al. 2012

Fertility and Sterility

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Orthotopic transplantation of LH receptor knockout and wild-type ovaries

Cited by 11 publications

References 29 publications

Evidence for cross-talk between the LH receptor and LH during implantation in mice

Evidence for cross-talk between the LH receptor and LH during implantation in mice

Extragonadal LH/hCG action—Not yet time to rewrite textbooks

The impairment of reproduction in db/db mice is not mediated by intraovarian defective leptin signaling

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