Orthotopic transplantation of neonatal GFP rat ovary as experimental model to study ovarian development and toxicology

Marano, Jason; Sun, Dongming; Zama, Aparna Mahakali; Young, Wise; Uzumcu, Mehmet

doi:10.1016/j.reprotox.2008.09.001

Cited by 13 publications

(13 citation statements)

References 25 publications

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“…Following the same protocol, we isolated 17 v-myc transduced clones from E14.5 dorsal (Ctx) and ventral (GE) forebrains from GFP + Fischer rats (Marano et al, 2008). The progenitor cell differentiation protocol has been described previously (Li et al, 2008).…”

Section: Methodsmentioning

confidence: 99%

Isolation of a novel rat neural progenitor clone that expresses dlx family transcription factors and gives rise to functional gabaergic neurons in culture

Hader

Han

et al. 2012

Developmental Neurobiology

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GABAergic interneurons are lost in conditions including epilepsy and CNS injury, but there are few culture models available to study their function. Towards the goal of obtaining renewable sources of GABAergic neurons, we used the molecular profile of a functionally-incomplete GABAergic precursor clone to screen 17 new clones isolated from GFP+ rat E14.5 cortex and ganglionic eminence (GE) that were generated by viral introduction of v-myc. The clones grow as neurospheres in medium with FGF2, and after withdrawal of FGF2 they exhibit varying patterns of differentiation. Transcriptional profiling and qPCR indicated that one clone (GE6) expresses high levels of mRNAs encoding Dlx1, 2, 5 and 6, glutamate decarboxylases, and presynaptic proteins including neuropeptide Y and somatostatin. Protein expression confirmed that GE6 is a progenitor with restricted differentiation giving rise mostly to neurons with GABAergic markers. In co-cultures with hippocampal neurons, GE6 neurons became electrically excitable and received both inhibitory and excitatory synapses. After withdrawal of FGF2 in cultures of GE6 alone, neurons matured to express βIII-tubulin, and staining for synaptophysin and vesicular GABA transporter (VGAT) were robust after 1-2 weeks of differentiation. GE6 neurons also became electrically excitable and displayed synaptic activity, but synaptic currents were carried by chloride and were blocked by bicuculline. The results suggest that the GE6 clone, which is ventrally derived from the GE, resembles GABAergic interneuron progenitors that migrate into the developing forebrain. This is the first report of a relatively stable fetal clone that can be differentiated into GABAergic interneurons with functional synapses.

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Section: Methodsmentioning

confidence: 99%

Isolation of a novel rat neural progenitor clone that expresses dlx family transcription factors and gives rise to functional gabaergic neurons in culture

Hader

Han

et al. 2012

Developmental Neurobiology

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“…The GFP transgenic Sprague-Dawley rats (SD-Tg (CAG-EGFP) CZ-004Osb rats) were provided kindly by Dr. Okabe, University of Osaka, Japan. This transgenic strain carries the enhanced green fluorescent protein gene driven by ubiquitous CAG (CMV early enhancer/chicken β-actin (CAG) promoter [12,13]. All surgical interventions and postoperative animal care were provided in accordance with the Guide for the Care and Use of Laboratory Animals (National Research Council, 1996) and the Guidelines and Policies for Rodent Survival Surgery provided by the Animal Care and Use Committees of Shanghai Jiaotong University.…”

Section: Animal Carementioning

confidence: 99%

“…In this study, we adopted GFP transgenic Sprague-Dawley rat as the source of NSCs [12,13]. After transplantation into injured spinal cords of Wistar rats, the GFP-NSCs could be tracked over 1 year, which allowed us to observe their long-term fate, including survival, migration, differentiation and functional significance in vivo.…”

Section: Introductionmentioning

confidence: 99%

Long-term Fate of Allogeneic Neural Stem Cells Following Transplantation into Injured Spinal Cord

Lü

et al. 2009

Stem Cell Rev and Rep

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To characterize the fate of allogeneic neural stem cells (NSCs) following transplantation into injured spinal cord, green fluorescent protein (GFP)-NSCs isolated from GFP transgenic Sprague-Dawley rat embryos were transplanted into contused spinal cords of Wistar rats. The GFP-NSCs survived for at least 6 months in injured spinal cord; most of them differentiated rapidly into astrocytes, and a few were able to undergo proliferation. After transplantation, the GFP-NSCs remained in the transplantation site at the early stage, and then migrated along white-matter, and gathered around the injured cavity. At 6 months post-transplantation, CD8 T-lymphocytes infiltrated the spinal cord, and mixed lymphocyte culture from host and donor showed that lymphocytes from the host spleen were primed by allogeneic GFP-NSCs. At 12 months post-transplantation, most GFP cells in the spinal cord lost their morphology and disintegrated. The Basso, Beattie and Bresnahan score and footprint analysis indicated that the improvement of locomotor function in transplanted rats appeared only at the early stage, and was not seen even at 6 months after transplantation All these results suggest that the allogeneic NSCs, after transplantation into injured spinal cord, activate the host immune system. Therefore, if immunosuppressive agents are not used, the grafted allogeneic NSCs, although they can survive for a long time, are subjected to host immune rejection, and the effect of NSCs on functional recovery is limited.

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“…To further assess the endothelial functionality of the rOM-derived EP-like cells, we have investigated their tube formation potential on Matrigel. In this assay, we also included rOM-derived EP-like cells isolated from GFP transgenic rats [39] (GrOM-EPCs) (Fig. 4A), HUVECs, RAECs, and hDP-MSCs (Fig.…”

Section: Resultsmentioning

confidence: 99%

Oral Mucosa Harbors a High Frequency of Endothelial Cells: A Novel Postnatal Cell Source for Angiogenic Regeneration

Zhou

Rogers

Lee

et al. 2017

Stem Cells and Development

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Endothelial progenitor cells/endothelial cells (EPCs/ECs) have great potential to treat pathological conditions such as cardiac infarction, muscle ischemia, and bone fractures, but isolation of EPC/ECs from existing cell sources is challenging due to their low EC frequency. We have isolated endothelial progenitor (EP)-like cells from rat oral mucosa and characterized their yield, immunophenotype, growth, and in vivo angiogenic potential. The frequency of EP-like cells derived from oral mucosa is thousands of folds higher than EPCs derived from donor-match bone marrow samples. EP-like cells from oral mucosa were positive for EC markers CD31, VECadherin, and VEGFR2. Oral mucosa-derived EP-like cells displayed robust uptake of acetylated low-density lipoprotein and formed stable capillary networks in Matrigel. Subcutaneously implanted oral mucosa-derived EP-like cells anastomosed with host blood vessels, implicating their ability to elicit angiogenesis. Similar to endothelial colony-forming cells, EP-like cells from oral mucosa have a significantly higher proliferative rate than human umbilical vein endothelial cells. These findings identify a putative EPC source that is easily accessible in the oral cavity, potentially from discarded tissue specimens, and yet with robust yield and potency for angiogenesis in tissue and organ regeneration.

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Orthotopic transplantation of neonatal GFP rat ovary as experimental model to study ovarian development and toxicology

Cited by 13 publications

References 25 publications

Isolation of a novel rat neural progenitor clone that expresses dlx family transcription factors and gives rise to functional gabaergic neurons in culture

Isolation of a novel rat neural progenitor clone that expresses dlx family transcription factors and gives rise to functional gabaergic neurons in culture

Long-term Fate of Allogeneic Neural Stem Cells Following Transplantation into Injured Spinal Cord

Oral Mucosa Harbors a High Frequency of Endothelial Cells: A Novel Postnatal Cell Source for Angiogenic Regeneration

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