OsAAP15, an amino acid transporter in response to nitrogen concentration, mediates panicle branching and grain yield in rice

Yang, Xiuyan; Yang, Guo; Wei, Xilin; Huang, Weiting; Fang, Zhongming

doi:10.1016/j.plantsci.2023.111640

Cited by 20 publications

(13 citation statements)

References 53 publications

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“…Additionally, it has been reported that OsAAP14 expression levels are regulated by exogenous basic amino acids (Yang et al, 2022). The expression of OsAAP15 is significantly up‐regulated under 0.5–15 mM N treatment (Yang et al, 2023). Therefore, the expression of some genes involved in the nitrogen pathway is affected by exogenous nitrogen treatment.…”

Section: Discussionmentioning

confidence: 99%

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Physiological and transcriptomic analysis provides novel insight into growth in Kam sweet rice under different nitrogen supplies

Amin,

Luo,

Shi

et al. 2024

Physiologia Plantarum

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Kam Sweet Rice (KSR) is a rice cultivar cultivated for thousands of years in China. Proper and balanced use of nitrogen sources is essential for achieving optimal growth and yield in KSR. This study was carried out to find the most effective hydroponic nutrient solution for cultivating and promoting the growth of KSR. Different nitrogen treatments were applied to KSR cultivars GouDan (GD), GouHa (GH), HongHe (HH), LeiLongGang (LG), and ZaiBianXiaoXiangHe (ZH), resulting in noticeable changes in both growth and physiological traits. Our findings revealed that D3 treatment [1.2 mM NH4Cl + 2.4 mM NaNO3+ neutral amino acids (0.1 mM Ser + 0.2 mM Thr + 0.2 mM Gly + 0.2 mM Pro +0.3 mM Ala)] had a notable positive impact on the growth of GH and ZH which showed the best performance in terms of plant height, root length and fresh weight when compared to the control (CK). Through transcriptome analysis, we discovered that these treatments altered the expression levels of genes associated with carbon metabolism, photosynthesis, and nitrogen pathways within 40 days. Interestingly, by altering the growth and tillering in rice transgenic plants, we validated the role of amino acid transporter OsAAP13 identified in transcriptome profiling, underscoring its significance. Our results are helpful for large‐scale production of KSR with the key methods for controlling the growth of KSR. It is a valuable asset for future investigations aimed at unravelling the intricate mechanisms governing plant growth in the context of different nitrogen supplies.

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Section: Discussionmentioning

confidence: 99%

“…OsAAP14 overexpression leads to increased tillers in rice subjected to melatonin concentrations (Yang et al, 2022). The response of OsAAP15 negatively influences panicle branching under high nitrogen conditions, affecting the levels of endogenous amino acids Tyr and Val in rice panicles (Yang et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Physiological and transcriptomic analysis provides novel insight into growth in Kam sweet rice under different nitrogen supplies

Amin,

Luo,

Shi

et al. 2024

Physiologia Plantarum

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“…Of course, the N concentration that promotes the panicle architecture needs to be maintained within a certain range, not the higher the better. Excessive N may even inhibit spike development (Yang et al ., 2023), possibly due to the imbalance between excessive N and other elements. Moreover, because of the different NUEs, the responses of panicle architecture with increasing N may be inconsistent among different rice varieties (Luo et al ., 2020; Liu et al ., 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Of course, the N concentration that promotes the panicle architecture needs to be maintained within a certain range, not the higher the better. Excessive N may even inhibit spike development (Yang et al, 2023) OsNLP3/4 performs overlapping but differential roles in panicle architecture regulation Both the OsNLP3-OE and OsNLP4-OE plants exhibited largerpanicle phenotypes, and the nlp3 and nlp4 mutants showed similar abnormal panicle architectures with shortened rachis, reduced branching and grain, due to regulation of the common OsRFL-OsMOC1 module (Figs 1-6, S2; Table S2), suggesting overlapping functions of OsNLP3/4 in rice panicle architecture regulation. However, OsNLP4 played a stronger role in panicle architecture than OsNLP3 (Figs 1, 2, S2; Table S2).…”

Section: New Phytologistmentioning

confidence: 99%

The OsNLP3/4‐OsRFL module regulates nitrogen‐promoted panicle architecture in rice

Wu,

Sun,

Song

et al. 2023

New Phytologist

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Summary Rice panicles, a major component of yield, are regulated by phytohormones and nutrients. How mineral nutrients promote panicle architecture remains largely unknown. Here, we report that NIN‐LIKE PROTEIN3 and 4 (OsNLP3/4) are crucial positive regulators of rice panicle architecture in response to nitrogen (N). Loss‐of‐function mutants of either OsNLP3 or OsNLP4 produced smaller panicles with reduced primary and secondary branches and fewer grains than wild‐type, whereas their overexpression plants showed the opposite phenotypes. The OsNLP3/4‐regulated panicle architecture was positively correlated with N availability. OsNLP3/4 directly bind to the promoter of OsRFL and activate its expression to promote inflorescence meristem development. Furthermore, OsRFL activates OsMOC1 expression by binding to its promoter. Our findings reveal the novel N‐responsive OsNLP3/4‐OsRFL‐OsMOC1 module that integrates N availability to regulate panicle architecture, shedding light on how N nutrient signals regulate panicle architecture and providing candidate targets for the improvement of crop yield.

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“…Amino acid transporter OsAAP14 determines bud outgrowth under melatonin treatment, thereby influencing rice tillering and grain yield [ 23 ]. Overexpressing of OsAAP15 in rice enhances both primary and secondary branches in the panicle, attributed to its impact on the concentrations of amino acids Tyr and Val in the rice panicle [ 24 ]. In maize, ZmAAP6 positively modulates grain protein [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Blocking of amino acid transporter OsAAP7 promoted tillering and yield by determining basic and neutral amino acids accumulation in rice

Jin,

Xie,

et al. 2024

BMC Plant Biol

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Background Amino acids are not only the main form of N in rice, but also are vital for its growth and development. These processes are facilitated by amino acid transporters within the plant. Despite their significance, only a few AAP amino acid transporters have been reported. Results In this study, we observed that there were differences in the expression of amino acid transporter OsAAP7 among 521 wild cultivated rice varieties, and it directly negatively correlated with tillering and grain yield per plant. We revealed that OsAAP7 protein was localized to the endoplasmic reticulum and had absorption and transport affinity for amino acids such as phenylalanine (Phe), lysine (Lys), leucine (Leu), and arginine (Arg) using subcellular localization, yeast substrate testing, fluorescent amino acid uptake, and amino acid content determination. Further hydroponic studies showed that exogenous application of amino acids Phe, Lys and Arg inhibited the growth of axillary buds in the overexpression lines, and promoted the elongation of axillary buds in the mutant lines. Finally, RNA-seq analysis showed that the expression patterns of genes related to nitrogen, auxin and cytokinin pathways were changed in axillary buds of OsAAP7 transgenic plants. Conclusions This study revealed the gene function of OsAAP7, and found that blocking of amino acid transporter OsAAP7 with CRISPR/Cas9 technology promoted tillering and yield by determining basic and neutral amino acids accumulation in rice.

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OsAAP15, an amino acid transporter in response to nitrogen concentration, mediates panicle branching and grain yield in rice

Cited by 20 publications

References 53 publications

Physiological and transcriptomic analysis provides novel insight into growth in Kam sweet rice under different nitrogen supplies

Physiological and transcriptomic analysis provides novel insight into growth in Kam sweet rice under different nitrogen supplies

The OsNLP3/4‐OsRFL module regulates nitrogen‐promoted panicle architecture in rice

Blocking of amino acid transporter OsAAP7 promoted tillering and yield by determining basic and neutral amino acids accumulation in rice

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