OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice (<i>Oryza sativa</i>)

Qi, Yanhua; Wang, Suikang; Shen, Chenjia; Zhang, SaiNa; Chen, Yue; Xu, Yan-Xia; Liu, Yu; Wu, Yunrong; Jiang, Dongxiang

doi:10.1111/j.1469-8137.2011.03910.x

Cited by 165 publications

(143 citation statements)

References 53 publications

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“…The miR167d targeted and negatively regulated auxin OsARF12 . When OsARF12 was knocked out, the expression of OsMIR , OsIRT1 , and OsSPR1 decreased, resulting in shortened roots, and decreased iron contents in the root tips [67]. In Arabidopsis thaliana , the same phenotype of miR169 was observed, indicating that miR169 plays a role in controlling root architecture [19].…”

Section: Discussionmentioning

confidence: 99%

Identification of microRNAs in Response to Drought in Common Wild Rice (Oryza rufipogon Griff.) Shoots and Roots

et al. 2017

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BackgroundDrought is the most important factor that limits rice production in drought-prone environments. Plant microRNAs (miRNAs) are involved in biotic and abiotic stress responses. Common wild rice (Oryza rufipogon Griff.) contains abundant drought-resistant genes, which provide an opportunity to explore these excellent resources as contributors to improve rice resistance, productivity, and quality.ResultsIn this study, we constructed four small RNA libraries, called CL and CR from PEG6000-free samples and DL and DR from PEG6000-treated samples, where ‘R’ indicates the root tissue and ‘L’ indicates the shoot tissue. A total of 200 miRNAs were identified to be differentially expressed under the drought-treated conditions (16% PEG6000 for 24 h), and the changes in the miRNA expression profile of the shoot were distinct from those of the root. At the miRNA level, 77 known miRNAs, which belong to 23 families, including 40 up-regulated and 37 down-regulated in the shoot, and 85 known miRNAs in 46 families, including 65 up-regulated and 20 down-regulated in the root, were identified as differentially expressed. In addition, we predicted 26 new miRNA candidates from the shoot and 43 from the root that were differentially expressed during the drought stress. The quantitative real-time PCR analysis results were consistent with high-throughput sequencing data. Moreover, 88 miRNAs that were differentially-expressed were predicted to match with 197 targets for drought-stress.ConclusionOur results suggest that the miRNAs of O. rufipogon are responsive to drought stress. The differentially expressed miRNAs that are tissue-specific under drought conditions could play different roles in the regulation of the auxin pathway, the flowering pathway, the drought pathway, and lateral root formation. Thus, the present study provides an account of tissue-specific miRNAs that are involved in the drought adaption of O. rufipogon.

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Section: Discussionmentioning

confidence: 99%

Identification of microRNAs in Response to Drought in Common Wild Rice (Oryza rufipogon Griff.) Shoots and Roots

et al. 2017

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“…Furthermore, we identified a potential AuxRE element in the promoter of OsCKX4. To investigate whether ARFs interact with the OsCKX4 promoter region, we performed yeast one-hybrid assays to test whether OsARF1, OsARF12, and OsARF25, three transcription factors known to act in regulating root development (Waller et al, 2002;Inukai et al, 2005;Qi et al, 2012), bind to the OsCKX4 promoter. Our results showed that only OsARF25 directly binds to the OsCKX4 promoter (Fig.…”

Section: Osckx4 Is Regulated By Auxin and Cytokinin Via Orrs And Osarmentioning

confidence: 99%

CYTOKININ OXIDASE/DEHYDROGENASE4 Integrates Cytokinin and Auxin Signaling to Control Rice Crown Root Formation

et al. 2014

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Crown roots constitute the majority of the rice (Oryza sativa) root system and play an important role in rice growth and development. However, the molecular mechanism of crown root formation in rice is not well understood. Here, we characterized a rice dominant mutant, root enhancer1 (ren1-D), which was observed to exhibit a more robust root system, increased crown root number, and reduced plant height. Molecular and genetic analyses revealed that these phenotypes are caused by the activation of a cytokinin oxidase/dehydrogenase (CKX) family gene, OsCKX4. Subcellular localization demonstrated that OsCKX4 is a cytosolic isoform of CKX. OsCKX4 is predominantly expressed in leaf blades and roots. It is the dominant CKX, preferentially expressed in the shoot base where crown root primordia are produced, underlining its role in root initiation. OsCKX4 is induced by exogenous auxin and cytokinin in the roots. Furthermore, one-hybrid assays revealed that OsCKX4 is a direct binding target of both the auxin response factor OsARF25 and the cytokinin response regulators OsRR2 and OsRR3. Overexpression and RNA interference of OsCKX4 confirmed that OsCKX4 plays a positive role in crown root formation. Moreover, expression analysis revealed a significant alteration in the expression of auxin-related genes in the ren1-D mutants, indicating that the OsCKX4 mediates crown root development by integrating the interaction between cytokinin and auxin. Transgenic plants harboring OsCKX4 under the control of the root-specific promoter RCc3 displayed enhanced root development without affecting their shoot parts, suggesting that this strategy could be a powerful tool in rice root engineering.

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“…Furthermore, previous works revealed the mechanisms of IAA-mediated gene expression, signaling and transport in the model dicot Arabidopsis (Leyser 2002;Paponov et al 2005). Cross-talk between auxin and Fe homeostasis suggests that auxin plays a role in the root morphology in response to Fe deficiency and Fe deficiency leads to increased auxin synthesis in Arabidopsis (Schmidt et al 2000;Chen et al 2010) and rice (Qi et al 2012;Shen et al 2015). Oxidative stress or increase in reactive oxygen species (ROS) is a consequence of several abiotic stresses.…”

Section: Introductionmentioning

confidence: 99%

Auxin signaling is closely associated with Zn-efficiency in rice (Oryza sativa L.)

Begum

Islam

Sarkar

et al. 2016

Journal of Plant Interactions

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This study elucidates the involvement of auxin with Zn-efficiency (ZE) in Zn-efficient rice var. Pokkali. Pokkali showed no significant decrease in morpho-physiological features, electrolyte leakage and total soluble proteins due to Zn deficiency as compared with Zn-sufficient seedlings. However, auxin inhibitor under Zn deficiency severely affected these characteristics, suggesting that ZE is associated with auxin signaling in rice. Results further revealed significant decreases in the expression of Zn transporter genes (OsIRT1, OsZIP4 and OsZIP1), OsDMAS1 (deoxymugeneic acid synthase) and phytochelatin in roots due to auxin inhibitor. It implies that auxin signaling may trigger Zn uptake, transport and chelation in rice seedlings to withstand Zn-deficiency. Further, significant reduction of major S-metabolites (cysteine, methionine, glutathione) and antioxidant enzymes (superoxide dismutase and glutathione reductase) along with increased H 2 O 2 content, due to auxin inhibitor under Zn deficiency compared with controls. Taken together, these findings reveal that mechanisms associated with ZE in Pokkali are dependent on auxin signaling. ARTICLE HISTORY

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OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice (Oryza sativa)

Cited by 165 publications

References 53 publications

Identification of microRNAs in Response to Drought in Common Wild Rice (Oryza rufipogon Griff.) Shoots and Roots

Identification of microRNAs in Response to Drought in Common Wild Rice (Oryza rufipogon Griff.) Shoots and Roots

CYTOKININ OXIDASE/DEHYDROGENASE4 Integrates Cytokinin and Auxin Signaling to Control Rice Crown Root Formation

Auxin signaling is closely associated with Zn-efficiency in rice (Oryza sativa L.)

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