OsbZIP42 is a positive regulator of ABA signaling and confers drought tolerance to rice

Joo, Joungsu; Lee, Youn Hab; Song, Sang Ik

doi:10.1007/s00425-019-03104-7

Cited by 75 publications

(39 citation statements)

References 49 publications

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“…The bZIP TF family is a plant-specific transcription factor family and is involved in diverse signaling pathways underlying biotic and abiotic stress responses and biological processes. Although the genome-wide analysis of the bZIP transcription factor family has been conducted in soybean [17] and there are several bZIP genes' functions reported in plants [26,27], little is known about the functions of bZIP genes in soybean. In our study, GmbZIP19 was isolated from full-length soybean cDNA.…”

Section: Discussionmentioning

confidence: 99%

A Soybean bZIP Transcription Factor GmbZIP19 Confers Multiple Biotic and Abiotic Stress Responses in Plant

Cai

Bai

et al. 2020

IJMS

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The basic leucine zipper (bZIP) is a plant-specific transcription factor family that plays crucial roles in response to biotic and abiotic stresses. However, little is known about the function of bZIP genes in soybean. In this study, we isolated a bZIP gene, GmbZIP19, from soybean. A subcellular localization study of GmbZIP19 revealed its nucleus localization. We showed that GmbZIP19 expression was significantly induced by ABA (abscisic acid), JA (jasmonic acid) and SA (salicylic acid), but reduced under salt and drought stress conditions. Further, GmbZIP19 overexpression Arabidopsis lines showed increased resistance to S. sclerotiorum and Pseudomonas syringae associated with upregulated ABA-, JA-, ETH- (ethephon-)and SA-induced marker genes expression, but exhibited sensitivity to salt and drought stresses in association with destroyed stomatal closure and downregulated the salt and drought stresses marker genes’ expression. We generated a soybean transient GmbZIP19 overexpression line, performed a Chromatin immunoprecipitation assay and found that GmbZIP19 bound to promoters of ABA-, JA-, ETH-, and SA-induced marker genes in soybean. The yeast one-hybrid verified the combination. The current study suggested that GmbZIP19 is a positive regulator of pathogen resistance and a negative regulator of salt and drought stress tolerance.

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Section: Discussionmentioning

confidence: 99%

A Soybean bZIP Transcription Factor GmbZIP19 Confers Multiple Biotic and Abiotic Stress Responses in Plant

Cai

Bai

et al. 2020

IJMS

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“…Since rice is most susceptible to drought and heat stress at reproductive stage, thus this could be a potential TF regulating drought tolerance in rice at reproductive stage [1,30]. In addition, Homeodomain, MYB, bZIP and C2H2 TFs were shown to be involved in drought tolerance in crop plants by modulating the expression of several genes [31-33]. Here, we report novel drought responsive TFs belonging to blue module, including Homeodomain protein (LOC_Os04g49450), MYB (LOC_Os05g04210 and LOC_Os04g43680), bZIP (LOC_Os06g10880) and C2H2 (LOC_Os03g32230) which could be functionally characterized using systems biology approaches to improve our understanding of the molecular mechanism of drought tolerance.…”

Section: Discussionmentioning

confidence: 99%

Identification of putative drought-responsive genes in rice using gene co-expression analysis

Dossa³

et al. 2019

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Drought is one of the major abiotic stresses causing yield losses and restricted growing area for several major crops. Rice being a major staple food crop and sensitive to water-deficit conditions bears heavy yield losses due to drought stress. To breed drought tolerant rice cultivars, it is of interest to understand the mechanisms of drought tolerance. In this regard, large amount of publicly available transcriptome datasets could be utilized. In this study, we used different transcriptome datasets obtained under drought stress in comparison to normal conditions (control) to identify novel drought responsive genes and their underlying molecular mechanisms. We found 517 core drought responsive differentially expressed genes (DEGs) and different modules using gene co-expression analysis to specifically predict their biological roles in drought tolerance. Gene ontology and KEGG analyses showed key biological processes and metabolic pathways involved in drought tolerance. Further, network analysis pinpointed important hub DEGs and major transcription factors regulating the expression of drought responsive genes in each module. These identified novel DEGs and transcription factors could be functionally characterized using systems biology approaches, which can significantly enhance our knowledge about the molecular mechanisms of drought tolerance in rice.

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“…To identify the potential target genes regulated by ZmBES1/BZR1-5, the 2000 bp upstream of DEGs were retrieved using the sequence data in the TAIR10 genome release and used to search for E-box or BRRE elements. The results of the promoter analysis showed there were abundant E-box elements (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) in the promoter regions of the 30 stress-related DEGs (Table S2), indicating that ZmBES1/BZR1-5 might bind to these E-box elements to regulate their expression. Hence, a yeast-one hybrid (Y1H) assay was performed to test whether ZmBES1/BZR1-5 binds to the E-box elements.…”

Section: Zmbes1/bzr1-5 Regulates the Expression Of Stress-related Genesmentioning

confidence: 99%

“…For instance, as the root is the first defensive line for soilborne stresses, including water deficits and high salt, plants can resist these stresses by adjusting their root system architecture [7,8]. Moreover, phytohormones and transcription factors regulate the expression of stress-induced genes, and the resulting signal transduction cascade can help plants survive under adverse conditions [9,10].…”

Section: Introductionmentioning

confidence: 99%

Maize ZmBES1/BZR1-5 Decreases ABA Sensitivity and Confers Tolerance to Osmotic Stress in Transgenic Arabidopsis

Sun

et al. 2020

IJMS

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The BRI1-EMS suppressor 1 (BES1)/brassinazole-resistant 1 (BZR1) transcription factors, key components in the brassinosteroid signaling pathway, play pivotal roles in plant growth and development. However, the function of BES1/BZR1 in crops during stress response remains poorly understood. In the present study, we characterized ZmBES1/BZR1-5 from maize, which was localized to the nucleus and was responsive to abscisic acid (ABA), salt and drought stresses. Heterologous expression of ZmBES1/BZR1-5 in transgenic Arabidopsis resulted in decreased ABA sensitivity, facilitated shoot growth and root development, and enhanced salt and drought tolerance with lower malondialdehyde (MDA) content and relative electrolyte leakage (REL) under osmotic stress. The RNA sequencing (RNA-seq) analysis revealed that 84 common differentially expressed genes (DEGs) were regulated by ZmBES1/BZR1-5 in transgenic Arabidopsis. Subsequently, gene ontology and KEGG pathway enrichment analyses showed that the DEGs were enriched in response to stress, secondary metabolism and metabolic pathways. Furthermore, 30 DEGs were assigned to stress response and possessed 2–15 E-box elements in their promoters, which could be potentially recognized and bound by ZmBES1/BZR1-5. Taken together, our results reveal that the ZmBES1/BZR1-5 transcription factor positively regulates salt and drought tolerance by binding to E-box to induce the expression of downstream stress-related genes. Therefore, our study contributes to the better understanding of BES1/BZR1 function in the stress response of plants.

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OsbZIP42 is a positive regulator of ABA signaling and confers drought tolerance to rice

Cited by 75 publications

References 49 publications

A Soybean bZIP Transcription Factor GmbZIP19 Confers Multiple Biotic and Abiotic Stress Responses in Plant

A Soybean bZIP Transcription Factor GmbZIP19 Confers Multiple Biotic and Abiotic Stress Responses in Plant

Identification of putative drought-responsive genes in rice using gene co-expression analysis

Maize ZmBES1/BZR1-5 Decreases ABA Sensitivity and Confers Tolerance to Osmotic Stress in Transgenic Arabidopsis

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