Oscillatory inertial focusing in infinite microchannels

Mutlu, Baris R.; F., Jon; Toner, Mehmet

doi:10.1073/pnas.1721420115

Cited by 78 publications

(70 citation statements)

References 30 publications

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“…However, an oscillatory flow strategy enables microchannels to have an infinite effective length, enabling the inertial focusing of small particles in relatively short channels. This was demonstrated by Mutlu et al in 2017, who used a 10–20 Hz oscillatory flow to focus a variety of particles as small as 500 nm in 10 s . In addition to focusing, other modes of self‐assembly are possible in oscillatory inertial microfluidic systems …”

Section: Pulsatile Flows In Microfluidic Processesmentioning

confidence: 99%

Pulsatile Flow in Microfluidic Systems

Dincau

Dressaire

Sauret

2019

Small

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This review describes the current knowledge and applications of pulsatile flow in microfluidic systems. Elements of fluid dynamics at low Reynolds number are first described in the context of pulsatile flow. Then the practical applications in microfluidic processes are presented: the methods to generate a pulsatile flow, the generation of emulsion droplets through harmonic flow rate perturbation, the applications in mixing and particle separation, and the benefits of pulsatile flow for clog mitigation. The second part of the review is devoted to pulsatile flow in biological applications. Pulsatile flows can be used for mimicking physiological systems, to alter or enhance cell cultures, and for bioassay automation. Pulsatile flows offer unique advantages over a steady flow, especially in microfluidic systems, but also require some new physical insights and more rigorous investigation to fully benefit future applications.

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Section: Pulsatile Flows In Microfluidic Processesmentioning

confidence: 99%

Pulsatile Flow in Microfluidic Systems

Dincau

Dressaire

Sauret

2019

Small

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“…Due to the symmetry of the velocity field, the particles preserve their motion direction, which allows for continuous separation like in infinite channels. For instance, Mutlu et al demonstrated the successful isolation of submicron particles and Staphylococcus aureus bacteria exploiting this approach [311]. However, the preparation of curved and wave-shaped microchannels might be challenging, and, for some applications, the straight microchannels are preferable due to the ease of preparation and parallelization.…”

Section: Inertial Focusing In Microfluidic Channelsmentioning

confidence: 99%

Section: Inertial Focusing In Microfluidic Channelsmentioning

confidence: 99%

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

Voronin

Kozlova

Verkhovskii

et al. 2020

IJMS

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Flow cytometry nowadays is among the main working instruments in modern biology paving the way for clinics to provide early, quick, and reliable diagnostics of many blood-related diseases. The major problem for clinical applications is the detection of rare pathogenic objects in patient blood. These objects can be circulating tumor cells, very rare during the early stages of cancer development, various microorganisms and parasites in the blood during acute blood infections. All of these rare diagnostic objects can be detected and identified very rapidly to save a patient’s life. This review outlines the main techniques of visualization of rare objects in the blood flow, methods for extraction of such objects from the blood flow for further investigations and new approaches to identify the objects automatically with the modern deep learning methods.

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“…Recently, oscillatory flows have been used to eliminate the need for long microchannel lengths and successfully applied to the inertial focusing of particles down to 500 nm (49). However, the use of oscillatory flows to manipulate species below this size is still unexplored.…”

Section: Introductionmentioning

confidence: 99%

“…However, the use of oscillatory flows to manipulate species below this size is still unexplored. Mutlu et al have demonstrated that oscillatory inertial microfluidics can be used to focus particles in low Reynolds number regimes (one order of magnitude less than steady-flow inertial microfluidics) (49), however, the efficient focusing of species with dimensions below 100 nm would require microchannels with reduced dimensional cross-sections and channel lengths (on the order of few millimeters or microns) to allow access to high flow velocities. Although, such structures have been fabricated in rigid epoxy (42) or silicon (50) for example, system complexity and cost are unavoidably increased.…”

Section: Introductionmentioning

confidence: 99%

Oscillatory Viscoelastic Microfluidics for Efficient Focusing and Separation of Nanoscale Species

Asghari

Cao

Mateescu

et al. 2019

Preprint

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The ability to precisely control particle migration within microfluidic systems is essential for focusing, separating, counting and detecting a wide range of biological species. To date, viscoelastic microfluidic systems have primarily been applied to the focusing, separation and isolation of micron-sized species, with their use in nanoparticle manipulations being underdeveloped and underexplored, due to issues related to nanoparticle diffusivity and a need for extended channel lengths. To overcome such issues, we herein present sheathless oscillatory viscoelastic microfluidics as a method for focusing and separating both micron and sub-micron species. To highlight the efficacy of our approach, we segment our study into three size regimes, namely micron (where characteristic particle dimensions are above 1 µm), sub-micron (where characteristic dimensions are between 1 µm and 100 nm) and nano (where characteristic dimensions are below 100 nm) regimes. Based on the ability to successfully manipulate particles in all these regimes, we demonstrate the successful isolation of p-bodies from biofluids (in the micron regime), the focusing of λ-DNA (in the sub-micron regime) and the focusing of extracellular vesicles (in the nano-regime). Finally, we characterize the physics underlying viscoelastic microflows using a dimensionless number that relates the lateral velocity (due to elastic effects) to the diffusion constant of the species within the viscoelastic carrier fluid. Based on the ability to precisely manipulate species in all three regimes, we expect that sheathless oscillatory viscoelastic microfluidics will provide for significant new opportunities in a range of biological and life science applications.

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Oscillatory inertial focusing in infinite microchannels

Cited by 78 publications

References 30 publications

Pulsatile Flow in Microfluidic Systems

Pulsatile Flow in Microfluidic Systems

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

Oscillatory Viscoelastic Microfluidics for Efficient Focusing and Separation of Nanoscale Species

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