OsCUL3a Negatively Regulates Cell Death and Immunity by Degrading OsNPR1 in Rice

Liu, Qunen; Ning, Yuping; Yu, Ning; Zhao, Chunde; Zhan, Xiaodeng; Wu, Weixun; Chen, Daibo; Wei, Xiaosheng; Wang, Guo‐Liang; Cheng, Shifeng; Zhang, Yingxin

doi:10.1105/tpc.16.00650

Cited by 117 publications

(102 citation statements)

References 41 publications

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“…OsPBZ1 is a defense marker gene associated with the SA signaling pathway (Lu et al ., ). OsNPR1 is the rice homolog of AtNPR1 , the master regulator of Arabidopsis innate immunity (Chern et al ., , ; Liu et al ., ). Compared with the control lines, both OsPBZ1 and OsNPR1 were significantly repressed in miR164a‐OE, and to a lesser degree in the osnac60 rice at 48 hpi (Figure b).…”

Section: Resultsmentioning

confidence: 97%

Osa‐miR164a targets OsNAC60 and negatively regulates rice immunity against the blast fungus Magnaporthe oryzae

et al. 2018

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Exploring the regulatory mechanism played by endogenous rice miRNAs in defense responses against the blast disease is of great significance in both resistant variety breeding and disease control management. We identified rice defense-related miRNAs by comparing rice miRNA expression patterns before and after Magnaporthe oryzae strain Guy11 infection. We discovered that osa-miR164a expression reduced upon Guy11 infection at both early and late stages, which was perfectly associated with the induced expression of its target gene, OsNAC60. OsNAC60 encodes a transcription factor, over-expression of which enhanced defense responses, such as increased programmed cell death, greater ion leakage, more reactive oxygen species accumulation and callose deposition, and upregulation of defense-related genes. By using transgenic rice over-expressing osa-miR164a, and a transposon insertion mutant of OsNAC60, we showed that when the miR164a/OsNAC60 regulatory module was dysfunctional, rice developed significant susceptibility to Guy11 infection. The co-expression of OsNAC60 and osa-miR164a abolished the OsNAC60 activity, but not its synonymous mutant. We further validated that this regulatory module is conserved in plant resistance to multiple plant diseases, such as the rice sheath blight, tomato late blight, and soybean root and stem rot diseases. Our results demonstrate that the miR164a/OsNAC60 regulatory module manipulates rice defense responses to M. oryzae infection. This discovery is of great potential for resistant variety breeding and disease control to a broad spectrum of pathogens in the future.

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Section: Resultsmentioning

confidence: 97%

Osa‐miR164a targets OsNAC60 and negatively regulates rice immunity against the blast fungus Magnaporthe oryzae

et al. 2018

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“…The in vivo degradation assays were performed according to Liu et al (2017) with minor modifications. Equal amounts of FZP-Flag and GFP plasmids were co-transfected into TQ and nal1-4 protoplasts using a method described by He et al (2016).…”

Section: In Vivo Degradation Assaysmentioning

confidence: 99%

Variation in the regulatory region of FZP causes increases in secondary inflorescence branching and grain yield in rice domestication

Huang

Zhao

et al. 2018

The Plant Journal

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Inflorescence branching is a key agronomic trait determining rice yield. The primary branch of the ancestral wild rice (Oryza rufipogon Griff.) bears few grains, due to minimal secondary branching. By contrast, Oryza sativa cultivars have been selected to produce large panicles with more secondary branches. Here we showed that the CONTROL OF SECONDARY BRANCH 1 (COS1) gene, which is identical to FRIZZY PANICLE (FZP), plays an important role in the key transition from few secondary branches in wild rice to more secondary branches in domesticated rice cultivars. A 4-bp tandem repeat deletion approximately 2.7 kb upstream of FZP may affect the binding activities of auxin response factors to the FZP promoter, decrease the expression level of FZP and significantly enhance the number of secondary branches and grain yield in cultivated rice. Functional analyses showed that NARROW LEAF 1 (NAL1), a trypsin-like serine and cysteine protease, interacted with FZP and promoted its degradation. Consistently, downregulating FZP expression or upregulating NAL1 expression in the commercial cultivar Zhonghua 17 increased the number of secondary branches per panicle, grain number per panicle and grain yield per plant. Our findings not only provide insights into the molecular mechanism of increasing grain number and yield during rice domestication, but also offer favorable genes for improving the grain yield of rice.

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“…Protein ubiqutination has emerged as a key mechanism involved in growth and development as well as immunity in animals and plants (Liu et al ., ; You et al ., ). Recognition of ubiquitin or specific ubiquitin chains by ubiquitin‐binding domains (UBDs) is vital for deciphering ubiquitin‐mediated signalling pathways (Liu et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Disruption of gene SPL35, encoding a novel CUE domain‐containing protein, leads to cell death and enhanced disease response in rice

Wang

et al. 2019

Plant Biotechnology Journal

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Summary Lesion mimic mutants that exhibit spontaneous hypersensitive response ( HR )‐like necrotic lesions are ideal experimental systems for elucidating molecular mechanisms involved in plant cell death and defence responses. Here we report identification of a rice lesion mimic mutant, spotted leaf 35 ( spl35 ), and cloning of the causal gene by TAIL ‐ PCR strategy. spl35 exhibited decreased chlorophyll content, higher accumulation of H 2 O 2 , up‐regulated expression of defence‐related marker genes, and enhanced resistance to both fungal and bacterial pathogens of rice. The SPL 35 gene encodes a novel CUE (coupling of ubiquitin conjugation to ER degradation) domain‐containing protein that is predominantly localized in cytosol, ER and unknown punctate compartment(s). SPL 35 is constitutively expressed in all organs, and both overexpression and knockdown of SPL 35 cause the lesion mimic phenotype. SPL 35 directly interacts with the E2 protein Os UBC 5a and the coatomer subunit delta proteins Delta‐ COP 1 and Delta‐ COP 2 through the CUE domain, and down‐regulation of these interacting proteins also cause development of HR ‐like lesions resembling those in spl35 and activation of defence responses, indicating that SPL 35 may be involved in the ubiquitination and vesicular trafficking pathways. Our findings provide insight into a role of SPL 35 in regulating cell death and defence response in plants.

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OsCUL3a Negatively Regulates Cell Death and Immunity by Degrading OsNPR1 in Rice

Cited by 117 publications

References 41 publications

Osa‐miR164a targets OsNAC60 and negatively regulates rice immunity against the blast fungus Magnaporthe oryzae

Osa‐miR164a targets OsNAC60 and negatively regulates rice immunity against the blast fungus Magnaporthe oryzae

Variation in the regulatory region of FZP causes increases in secondary inflorescence branching and grain yield in rice domestication

Disruption of gene SPL35, encoding a novel CUE domain‐containing protein, leads to cell death and enhanced disease response in rice

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