OsLIS-L1 encoding a lissencephaly type-1-like protein with WD40 repeats is required for plant height and male gametophyte formation in rice

A differentially expressed transcript, encoding a putative WD protein (Setaria italica WD40; SiWD40), was identified in foxtail millet. Tertiary structure modeling revealed that its C-terminus possesses eight blade β-propeller architecture. Its N-terminal has three α-helices and two 3(10)-helices and was highly induced by different abiotic stresses. The SiWD40:GFP fusion protein was nuclear localized. Promoter analysis showed the presence of many cis-acting elements, including two dehydration responsive elements (DRE). A stress-responsive SiAP2 domain containing protein could specifically bind to these elements in the SiWD40 promoter. Thus, for the first time, we report that DREs probably regulate expression of SiWD40 during environmental stress. Molecular docking analysis revealed that the circumference of the β-propeller structure was involved in an interaction with a SiCullin4 protein, supporting the adaptability of SiWD40 to act as a scaffold. Our study thus provides a vital clue for near future research on the stress-regulation of WD proteins.

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“…5D-F). Our findings are corroborated by several previous studies reporting nuclear localization of plant WD40-repeat proteins[15,31,32].…”

supporting

confidence: 95%

The DNA-binding activity of an AP2 protein is involved in transcriptional regulation of a stress-responsive gene, SiWD40, in foxtail millet

et al. 2012

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“…There are three TPL/TPR proteins in rice, namely TPR1(LOC_Os01g15020), TPR2 (LOC_Os08g06480) and TPR3 (LOC_Os03g14980). Among them, TPR2 (also known as ASP1, OsREL2 or OsLIS-L1) has been reported to function in tiller and spikelet development 40–42 . As shown in Fig.…”

Section: Interaction Of D53 With Tpr Proteinsmentioning

confidence: 99%

DWARF 53 acts as a repressor of strigolactone signalling in rice

Jiang

Liu

Xiong

et al. 2013

Nature

708

822

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Strigolactones (SLs) are a group of newly identified plant hormones that control plant shoot branching. SL signalling requires the hormone-dependent interaction of DWARF 14 (D14), a probable candidate SL receptor, with DWARF 3 (D3), an F-box component of the Skp–Cullin–F-box (SCF) E3 ubiquitin ligase complex. Here we report the characterization of a dominant SL-insensitive rice (Oryza sativa) mutant dwarf 53 (d53) and the cloning of D53, which encodes a substrate of the SCFD3 ubiquitination complex and functions as a repressor of SL signalling. Treatments with GR24, a synthetic SL analogue, cause D53 degradation via the proteasome in a manner that requires D14 and the SCFD3 ubiquitin ligase, whereas the dominant form of D53 is resistant to SL-mediated degradation. Moreover, D53 can interact with transcriptional co-repressors known as TOPLESS-RELATED PROTEINS. Our results suggest a model of SL signalling that involves SL-dependent degradation of the D53 repressor mediated by the D14–D3 complex.

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“…S11C). One of the three rice TPRs, TPR2-also termed ABERRANT SPIKELET AND PANICLE1 (ASP1) or Lissencephalytype-1-like1 (OsLIS-L1)-was involved in regulating panicle development (22,23). Yeast two-hybrid assay showed that SNB, OsIDS1, and OsTOE1 interacted with TPR1 and ASP1 in yeast, and a series of protein mutation and truncation assays showed that the EAR C motif of SNB, OsIDS1, and OsTOE1, and the LisH (lissencephaly type I-like homolog) domain of ASP1 were responsible for the interactions (Fig.…”

Section: Effects Of Mir172 and Its Target Genes On The Transition Of mentioning

confidence: 99%

Coordinated regulation of vegetative and reproductive branching in rice

Wang

Sun

Jin

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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218

192

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Grasses produce tiller and panicle branching at vegetative and reproductive stages; the branching patterns largely define the diversity of grasses and constitute a major determinant for grain yield of many cereals. Here we show that a spatiotemporally coordinated gene network consisting of the MicroRNA 156 (miR156/)miR529/ SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) and miR172/ APETALA2 (AP2) pathways regulates tiller and panicle branching in rice. SPL genes negatively control tillering, but positively regulate inflorescence meristem and spikelet transition. Underproduction or overproduction of SPLs reduces panicle branching, but by distinct mechanisms: miR156 and miR529 fine-tune the SPL levels for optimal panicle size. miR172 regulates spikelet transition by targeting AP2-like genes, which does not affect tillering, and the AP2-like proteins play the roles by interacting with TOPLESS-related proteins (TPRs). SPLs modulate panicle branching by directly regulating the miR172/ AP2 and PANICLE PHYTOMER2 (PAP2)/Rice TFL1/CEN homolog 1 (RCN1) pathways and also by integrating other regulators, most of which are not involved in tillering regulation. These findings may also have significant implications for understanding branching regulation of other grasses and for application in rice genetic improvement.he architecture of grasses is largely determined by the branching patterns. Tillers and inflorescence branches are produced at vegetative and reproductive stages, respectively, and their patterns greatly contribute to the diversity of grasses and constitute a major determinant of grain yield of major cereals.Rice branching has attracted much attention because of its importance in food production. Axillary buds produce tillers during the vegetative stage. However, only the early ones formed from the unelongated internodes outgrow as tillers, whereas later ones formed from the upper internodes remain dormant. After reproductive transition, the shoot apical meristem is converted to inflorescence meristem to produce panicle. Rice panicle morphology is largely determined by the timing of identity transition among the different types of meristems (SI Appendix, Fig. S1). Therefore, fine-tuning of meristem phase change at reproductive stage defines the size and architecture of the rice panicle (1).Many genes have been identified as regulators of rice branching. Generally, genes involved in axillary bud initiation control both vegetative and reproductive branching, whereas genes under axillary bud outgrowth have specific roles only at certain stages (2, 3). LAX PANICLE 1 (LAX1) and MONOCULM1 control axillary bud initiation; mutation in either of them results in reduction of both tiller and panicle branches (4, 5). Other genes such as Grain number, plant height, and heading date7 exclusively control panicle branching (6). As a third class, many genes, including Ideal Plant Architecture 1 (IPA1)/Wealthy Farmer's Panicle (WFP) and genes related to strigolactone, play opposite roles in tiller and panicle branches (7-9). Therefore...

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OsLIS-L1 encoding a lissencephaly type-1-like protein with WD40 repeats is required for plant height and male gametophyte formation in rice

Cited by 42 publications

References 61 publications

The DNA-binding activity of an AP2 protein is involved in transcriptional regulation of a stress-responsive gene, SiWD40, in foxtail millet

The DNA-binding activity of an AP2 protein is involved in transcriptional regulation of a stress-responsive gene, SiWD40, in foxtail millet

DWARF 53 acts as a repressor of strigolactone signalling in rice

Coordinated regulation of vegetative and reproductive branching in rice

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