OsLYP4 and OsLYP6 play critical roles in rice defense signal transduction

Liu, Bing; Li, Jianfeng; Ao, Ying; Li, Zhangqun; Li, Jun; Feng, Dan; Qi, Kangbiao; He, Yanming; Zeng, Liexian; Wang, Jinfa; Wang, Hongbin

doi:10.4161/psb.22980

Cited by 25 publications

(12 citation statements)

References 13 publications

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“…We were interested in examining the relationship between these three receptor proteins, which lack intracellular domains, especially in the presence or absence of MAMPs. Previously, we detected positive interactions between OsLYP4 and OsLYP6 and between OsLYP4 and CEBiP in yeast two‐hybrid assays (Liu et al ., 2013a). Therefore, we further analyzed these interactions using coIP assays in a transient rice protoplast expression system.…”

Section: Resultsmentioning

confidence: 99%

OsCERK1 and OsRLCK176 play important roles in peptidoglycan and chitin signaling in rice innate immunity

et al. 2014

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These authors contributed equally to this work. SUMMARYMicrobe-associated molecular pattern (MAMP)-triggered immunity plays critical roles in the basal resistance defense response in plants. Chitin and peptidoglycan (PGN) are major molecular patterns for fungi and bacteria, respectively. Two rice (Oryza sativa) lysin motif-containing proteins, OsLYP4 and OsLYP6, function as receptors that sense bacterial PGN and fungal chitin. These membrane receptors, which lack intracellular kinase domains, likely contain another component for transmembrane immune signal transduction. Here, we demonstrate that the rice LysM receptor-like kinase OsCERK1, a key component of the chitin elicitor signaling pathway, also plays an important role in PGN-triggered immunity in rice. Silencing of OsCERK1 suppressed PGN-induced (and chitin-induced) immunity responses, including reactive oxygen species generation, defense gene expression, and callose deposition, indicating that OsCERK1 is essential for both PGN and chitin signaling initiated by OsLYP4 and OsLYP6. OsLYP4 associated with OsLYP6 and the rice chitin receptor chitin oligosaccharide elicitor-binding protein (CEBiP) in the absence of PGN or chitin, and treatment with PGN or chitin led to their disassociation in vivo. OsCERK1 associated with OsLYP4 or OsLYP6 when induced by PGN but it associated with OsLYP4, OsLYP6, or CEBiP under chitin treatment, suggesting the presence of different patterns of ligand-induced heterooligomeric receptor complexes. Furthermore, the receptor-like cytoplasmic kinase OsRLCK176 functions downstream of OsCERK1 in the PGN and chitin signaling pathways, suggesting that these MAMPs share overlapping intracellular signaling components. Therefore, OsCERK1 plays dual roles in PGN and chitin signaling in rice innate immunity and as an adaptor involved in signal transduction at the plasma membrane in conjunction with OsLYP4 and OsLYP6.

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Section: Resultsmentioning

confidence: 99%

OsCERK1 and OsRLCK176 play important roles in peptidoglycan and chitin signaling in rice innate immunity

et al. 2014

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“…Functional studies of LysM-RLP and LysM-RLK suggest that these receptors can function as hetero-oligomers ( Kelly et al, 2017 ; Zipfel and Oldroyd, 2017 ; Desaki et al, 2018 ); for example, the hetero-oligomer of AtLYK5-AtCERK1 recognizes chitin in Arabidopsis ( Cao et al, 2014 ), OsCEBiP-OsCERK1 recognizes chitin in rice ( Shimizu et al, 2010 ; Hayafune et al, 2014 ), AtLYM1/3-AtCERK1 recognizes peptidoglycans in Arabidopsis ( Willmann et al, 2011 ), OsLYP4/6-OsCERK1 recognizes peptidoglycan and chitin in rice ( Liu et al, 2013 ; Ao et al, 2014 ), and LjNFR5-LjNFR1 recognizes Nod factor in L. japonicus ( Broghammer et al, 2012 ). Therefore, it is very likely that SlLYK12 pairs with SlLYK10, the ortholog of NFR5 in tomato ( Buendia et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Tomato LysM Receptor-Like Kinase SlLYK12 Is Involved in Arbuscular Mycorrhizal Symbiosis

et al. 2018

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Arbuscular mycorrhiza (AM) is a widespread symbiotic relationship between plants and fungi (Glomeromycota), which improves the supply of water and nutrients to host plants. AM symbiosis is set in motion by fungal chitooligosaccharides and lipochitooligosaccharides, which are perceived by plant-specific LysM-type receptor kinases (LYK). In rice this involves OsCERK1, a LYK also essential for chitin triggered innate immunity. In contrast in legumes, the CERK1 homologous gene experienced duplication events resulting in subfunctionalization. However, it remains unknown whether this subfunctionalization is legume-specific, or has occurred also in other dicot plant species. We identified four CERK1 homologs in tomato (SlLYK1, SlLYK11, SlLYK12, and SlLYK13) and investigated their roles in chitin signaling and AM symbiosis. We found that knockdown of SlLYK12 in tomato significantly reduced AM colonization, whereas chitin-induced responses were unaffected. In contrast, knockdown of SlLYK1 resulted in reduced responses to chitin, but did not alter responses to AM fungi. Moreover, ectopic overexpression of SlLYK1 and SlLYK13 in Nicotiana benthamiana induced cell death, whereas SlLYK12 overexpression did not. Based on our results and comparison with rice OsCERK1, we hypothesize that OsCERK1 orthologs in tomato underwent gene duplication, leading to the subfunctionalization of immunity and symbiosis.

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“…The association of CE with CEBiP leads to rice plant resistance to the blast fungus, M. oryzae; hence, improving the response to CE by engineering CEBiP enhances disease tolerance. On the other hand, the predicted structure of CEBiP does not include any functional intracellular domains for signaling through the plasma membrane into the cytoplasm (Kishimoto et al, (Liu et al, 2012(Liu et al, , 2013 OsLYP6 RLK Senses both fungal chitin and bacterial peptidoglycan (PGN) lysin motif (LysM)-containing proteins. OsLYP4 and OsLYP6, as dual functional PRRs sense bacterial peptidoglycan (PGN) and fungal chitin.…”

Section: Cebip and Oscerk1 Lysm Receptor Fragments For Ce Signaling mentioning

confidence: 97%

“…OsLYP4 and OsLYP6, as dual functional PRRs sense bacterial peptidoglycan (PGN) and fungal chitin. (Liu et al, 2012(Liu et al, , 2013 OsRLCK185…”

Section: Cebip and Oscerk1 Lysm Receptor Fragments For Ce Signaling mentioning

confidence: 99%

Toward understanding of rice innate immunity againstMagnaporthe oryzae

Azizi

Rafii

Abdullah

et al. 2014

Critical Reviews in Biotechnology

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The blast fungus, Magnaporthe oryzae, causes serious disease on a wide variety of grasses including rice, wheat and barley. The recognition of pathogens is an amazing ability of plants including strategies for displacing virulence effectors through the adaption of both conserved and variable pathogen elicitors. The pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and effector-triggered immunity (ETI) were reported as two main innate immune responses in plants, where PTI gives basal resistance and ETI confers durable resistance. The PTI consists of extracellular surface receptors that are able to recognize PAMPs. PAMPs detect microbial features such as fungal chitin that complete a vital function during the organism's life. In contrast, ETI is mediated by intracellular receptor molecules containing nucleotide-binding (NB) and leucine rich repeat (LRR) domains that specifically recognize effector proteins produced by the pathogen. To enhance crop resistance, understanding the host resistance mechanisms against pathogen infection strategies and having a deeper knowledge of innate immunity system are essential. This review summarizes the recent advances on the molecular mechanism of innate immunity systems of rice against M. oryzae. The discussion will be centered on the latest success reported in plant-pathogen interactions and integrated defense responses in rice.

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OsLYP4 and OsLYP6 play critical roles in rice defense signal transduction

Cited by 25 publications

References 13 publications

OsCERK1 and OsRLCK176 play important roles in peptidoglycan and chitin signaling in rice innate immunity

OsCERK1 and OsRLCK176 play important roles in peptidoglycan and chitin signaling in rice innate immunity

Tomato LysM Receptor-Like Kinase SlLYK12 Is Involved in Arbuscular Mycorrhizal Symbiosis

Toward understanding of rice innate immunity againstMagnaporthe oryzae

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