Osmium tetroxide as a histochemical and histological reagent

Adams, C. W. M.; Abdulla, Y.H.; Bayliss, O. B.

doi:10.1007/bf00281808

Cited by 52 publications

(16 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because an OsO 4 molecule stoichiometrically crosslinks to two carbon-carbon double bonds, lipid layer membranes including unsaturated fatty acids are strongly stained by OsO 4 . In addition, soluble substances with carbon-carbon double bonds can be accumulated by crosslinking with OsO 4 to form condensates (Adams et al, 1967). In the present study, these observations led to the hypothesis that the central densities are not due to protein molecules, but may be condensation products of fixatives and substances with carbon-carbon double bonds in GJVs.…”

Section: Preparation Conditions Influenced the Central Densitiesmentioning

confidence: 54%

Electron tomographic analysis of gap junctions in lateral giant fibers of crayfish

Ohta

Nishikawa

Hiroaki

et al. 2011

Journal of Structural Biology

View full text Add to dashboard Cite

Section: Preparation Conditions Influenced the Central Densitiesmentioning

confidence: 54%

Electron tomographic analysis of gap junctions in lateral giant fibers of crayfish

Ohta

Nishikawa

Hiroaki

et al. 2011

Journal of Structural Biology

View full text Add to dashboard Cite

“…Electron microscopy can be used to distinguish TGs synthesized from fatty acids of diVerent saturation Osmium tetroxide preferentially binds to the unsaturated bonds of fatty acids and imparts electron density observable with electron microscopy (Hayes et al 1963;Adams et al 1967). We considered that the lipid esters formed by diVerent fatty acids would have diVerent electron densities, in accordance with their number of unsaturated bonds.…”

Section: Resultsmentioning

confidence: 99%

“…In the present study, we developed a method that utilizes the reaction between unsaturated fatty acids and osmium tetroxide (Hayes et al 1963;Adams et al 1967), which Fig. 5 The electron density of LDs in 3T3-L1 adipocytes treated with DHA.…”

Section: Discussionmentioning

confidence: 99%

“…The method utilizes the high reactivity of osmium tetroxide with unsaturated fatty acids (Hayes et al 1963;Adams et al 1967). That is, by providing cells with polyunsaturated fatty acids, incorporation of newly synthesized esters can be observed as an increase in the electron density of the LD, with this measured quantitatively by image analysis.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Quantitative electron microscopy shows uniform incorporation of triglycerides into existing lipid droplets

Cheng

Fujita

Ohsaki

et al. 2009

Histochem Cell Biol

View full text Add to dashboard Cite

The lipid droplet (LD) is an organelle with a lipid ester core and a surface phospholipid monolayer. The mechanism of LD biogenesis is not well understood. The present study aimed to elucidate the LD growth process, for which we developed a new electron microscopic method that quantifies the proportion of existing and newly synthesized triglycerides in individual LDs. Our method takes advantage of the reactivity of unsaturated fatty acids and osmium tetroxide, which imparts LDs an electron density that reflects fatty acid composition. With this method, existing triglyceride-rich LDs in 3Y1 fibroblasts were observed to incorporate newly synthesized triglycerides at a highly uniform rate. This uniformity and its persistence even after microtubules were depolymerized suggest that triglycerides in fibroblasts are synthesized in the local vicinity of individual LDs and then incorporated. In contrast, LDs in 3T3-L1 adipocytes showed heterogeneity in the rate at which lipid esters were incorporated, indicating different mechanisms of LD growth in fibroblasts and adipocytes.

show abstract

“…After 30 min of incubation, the reaction was terminated by thorough rinsing. Subsequently, osmium staining was performed by incubating sections in 1% osmium solution (Bie-Berntsen, Copenhagen, Denmark) for 1 h (14). Finally, sections were counterstained with ethylene green, dehydrated, and mounted with Aquatex (Merck, Damstadt, Germany).…”

Section: Immunohistochemical Stainingmentioning

confidence: 99%

GLUT4 expression in human muscle fibres is not correlated with intracellular triglyceride (TG) content. Is TG a maker or a marker of insulin resistance?

Gaster

Ottosen²,

Vach

et al. 2003

APMIS

View full text Add to dashboard Cite

We have recently reported a progressive decline in the expression of glucose transporter isoform 4 (GLUT4) from control subjects through obese non-diabetics to obese type 2 diabetic subjects, indicating that the reduced GLUT4 in slow twitch fibres could be secondary to obesity. In this study we investigate the association of GLUT4 expression with the intracellular triglyceride (TG) content in the same muscle fibres and with plasma lipid parameters. We used histochemistry and stereology to study the relationship between TG content and GLUT4 expression in muscle fibres from obese, obese type 2 diabetic subjects, and young lean controls. TG density was significantly higher in slow compared to fast fibres in all studied subjects (p<0.05). We found an increased TG density in slow twitch fibres of obese diabetic subjects compared to obese (p<0.05) and lean controls (p<0.008). Intracellular TG densities in slow and fast fibres did not correlate with the corresponding GLUT4 density in the same fibres in our study groups (p>0.05). Plasma TG and FFA did not correlate with GLUT4 expression in slow or fast fibres (p>0.05). In conclusion, TG content was increased in diabetic slow fibres with a reduced GLUT4 expression. The GLUT4 expression was not associated with an increased intracellular triglyceride content or with increased plasma FFA levels. Thus, intracellular TG content and circulating FFA may not influence glucose transport directly through GLUT4 expression.

show abstract

Osmium tetroxide as a histochemical and histological reagent

Cited by 52 publications

References 32 publications

Electron tomographic analysis of gap junctions in lateral giant fibers of crayfish

Electron tomographic analysis of gap junctions in lateral giant fibers of crayfish

Quantitative electron microscopy shows uniform incorporation of triglycerides into existing lipid droplets

GLUT4 expression in human muscle fibres is not correlated with intracellular triglyceride (TG) content. Is TG a maker or a marker of insulin resistance?

Contact Info

Product

Resources

About