OsMKKK70 regulates grain size and leaf angle in rice through the OsMKK4‐OsMAPK6‐OsWRKY53 signaling pathway

Liu, Zhiqi; Mei, Enyang; Tian, Xiaojie; He, Ming; Tang, Jufen; Xu, Min; Liu, Jiali; Lu, Song; Li, Xiufeng; Wang, Zhenyu; Guan, Qing; Xu, Qinying; Bu, Qingyun

doi:10.1111/jipb.13174

Cited by 32 publications

(28 citation statements)

References 46 publications

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“…In addition, oswrky53 mutant plants have decreased brassinosteroid (BR) sensitivity, whereas overexpressing OsWRKY53 leads to hypersensitivity to BR, and the phosphorylation of OsWRKY53 by OsMPK6 is required for its function in regulating BR responses. Recently, the same laboratory also demonstrated that OsMKKK70, together with OsMKKK55 and OsMKKK62 (orthologs of Arabidopsis MPKKK15/16/17/18), regulate grain size and leaf angle in rice through the OsMKK4–OsMAPK6–OsWRKY53 signaling module (Liu et al, 2021b). Compared to the wild type, the osmkkk62/70 double and osmkkk55/62/ 70 triple mutant plants set much smaller seeds and have a more erect leaf angle, whereas the overexpression of OsMKKK70 leads to bigger/longer seeds.…”

Section: Mapk Cascades In Plant Growth and Developmentmentioning

confidence: 99%

Mitogen‐activated protein kinase cascades in plant signaling

Zhang

2022

JIPB

266

119

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Mitogen-activated protein kinase (MAPK) cascades are key signaling modules downstream of receptors/sensors that perceive either endogenously produced stimuli such as peptide ligands and damage-associated molecular patterns (DAMPs) or exogenously originated stimuli such as pathogen/microbe-associated molecular patterns (P/MAMPs), pathogen-derived effectors, and environmental factors. In this review, we provide a historic view of plant MAPK research and summarize recent advances in the establishment of MAPK cascades as essential components in plant immunity, response to environmental stresses, and normal growth and development. Each tier of the MAPK cascades is encoded by a small gene family, and multi ple members can function redundantly in an MAPK cascade. Yet, they carry out a diverse array of biological functions in plants. How the signaling specificity is achieved has become an interesting topic of MAPK research. Future investigations into the molecular mechanism(s) underlying the regulation of MAPK activation including the activation kinetics and magnitude in response to a stimulus, the spatiotemporal expression patterns of all the components in the signaling pathway, and functional characterization of novel MAPK substrates are central to our understanding of MAPK functions and signaling specificity in plants.

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Section: Mapk Cascades In Plant Growth and Developmentmentioning

confidence: 99%

Mitogen‐activated protein kinase cascades in plant signaling

Zhang

2022

JIPB

266

119

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“…In rice plants, the OsMKKK70-OsMKK4-OsMPK6 cascade regulates grain size and leaf angle [ 60 ]. In this signaling cascade, OsMKKK70 (a MEKK-like MAPKKK) shows in vitro kinase activity and physically interacts with its downstream kinase, OsMKK4, in yeast and tobacco cells, but does not phosphorylate OsMKK4 ( Table 2 ).…”

Section: Uncanonical Regulation Of Mapk Cascades By Mapkkksmentioning

confidence: 99%

“…As OsMKKK70 does not interact with OsMPK6, it is unlikely that OsMKKK70 functions as a scaffold protein tethering both OsMKK4 and OsMPK6 to promote OsMPK6 phosphorylation by OsMKK4. Thus, whether the OsMKKK70 kinase activity is involved in the regulation of the OsMKK4-OsMPK6 cascade and how OsMKK4-OsMPK6 is activated have to be further studied [ 60 ].…”

Section: Uncanonical Regulation Of Mapk Cascades By Mapkkksmentioning

confidence: 99%

“…However, it is difficult to conclude that OsILA1 phosphorylates and, in turn, destabilizes OsMPKK4. Acute observations are required to determine whether and why OsILA1 phosphorylates or destabilizes OsMPKK4 in rice cells without extracellular stimulus, as OsMPKK4 is required for rice plant development, including grain size formation [ 60 , 86 , 87 , 88 ]. Presumably, OsMPKK4 may coordinate rice plant development and responses to environmental stimuli via phosphorylation by different protein kinases.…”

Section: Uncanonical Regulation Of Mapk Cascades By Mapkkksmentioning

confidence: 99%

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Regulatory Mechanisms of Mitogen-Activated Protein Kinase Cascades in Plants: More than Sequential Phosphorylation

Gao

Wang

et al. 2022

IJMS

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Mitogen-activated protein kinase (MAPK) cascades play crucial roles in almost all biological processes in plants. They transduce extracellular cues into cells, typically through linear and sequential phosphorylation and activation of members of the signaling cascades. However, accumulating data suggest various regulatory mechanisms of plant MAPK cascades in addition to the traditional phosphorylation pathway, in concert with their large numbers and coordinated roles in plant responses to complex ectocytic signals. Here, we highlight recent studies that describe the uncanonical mechanism of regulation of MAPK cascades, regarding the activation of each tier of the signaling cascades. More particularly, we discuss the unusual role for MAPK kinase kinases (MAPKKKs) in the regulation of MAPK cascades, as accumulating data suggest the non-MAPKKK function of many MAPKKKs. In addition, future work on the biochemical activation of MAPK members that needs attention will be discussed.

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“…Similarly, banana MaMPK14 enhanced MaBZR1/2-mediated transcriptional repression of fruit ripening-related genes [ 9 ], while MaMPK6–3 phosphorylates MabZIP21, which in turn increases its transcriptional activation of ripening-associated genes [ 10 ]. Recently, rice OsMKKK70 participates in the OsMKK4-OsMAPK6-OsWRKY53 signaling pathway to regulate leaf angle and grain size [ 11 ]. FaMAPK5 and FaMAPK10 are related to ABA-mediated fruit ripening in strawberry [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Mitogen-activated protein kinase 14-mediated phosphorylation of MaMYB4 negatively regulates banana fruit ripening

Yang

Shan

et al. 2022

Horticulture Research

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Mitogen-activated protein kinase (MAPK/MPK) cascades play crucial parts in plant growth, development processes, immune ability, and stress responses, however, the regulatory mechanism by which MAPK affects fruit ripening remains largely unexplored. Here, we reported that MaMPK14 cooperated with MaMYB4 to mediate postharvest banana fruit ripening. Transient overexpression of individual MaMPK14 and MaMYB4 in banana fruit delayed fruit ripening, confirming the negative roles in the ripening. The ripening negative regulator MaMYB4 could repress the transcription of genes associated with ethylene biosynthesis and fruit softening, such as MaACS1, MaXTH5, MaPG3 and MaEXPA15. Furthermore, MaMPK14 phosphorylated MaMYB4 at Ser160 via a direct interaction. Mutation at Ser160 of MaMYB4 reduced its interaction with MaMPK14 but did not affect its subcellular localization. Importantly, phosphorylation of MaMYB4 by MaMPK14 enhanced the MaMYB4-mediated transcriptional inhibition, binding strength, protein stability and the repression of fruit ripening. Taken together, our results delineated the regulation pathway of MAPK module during banana fruit ripening, which involved the phosphorylation modification of MaMYB4 mediated by MaMPK14.

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OsMKKK70 regulates grain size and leaf angle in rice through the OsMKK4‐OsMAPK6‐OsWRKY53 signaling pathway

Cited by 32 publications

References 46 publications

Mitogen‐activated protein kinase cascades in plant signaling

Mitogen‐activated protein kinase cascades in plant signaling

Regulatory Mechanisms of Mitogen-Activated Protein Kinase Cascades in Plants: More than Sequential Phosphorylation

Mitogen-activated protein kinase 14-mediated phosphorylation of MaMYB4 negatively regulates banana fruit ripening

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