Osmo-Sensitive and Stretch-Activated Calcium-Permeable Channels in <i>Vicia faba</i> Guard Cells Are Regulated by Actin Dynamics

Zhang, Wei; Fan, Liu‐Min; Wu, Weihua

doi:10.1104/pp.106.091405

Cited by 84 publications

(96 citation statements)

References 58 publications

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“…This offers a potential explanation for the functional relevance of branched F‐actin. It has long been established that the response of membrane‐integrated stress‐responsive calcium channels requires a dynamic actin cytoskeleton (Wang et al ., 2004; Zhang et al ., 2007). It seems increasingly likely that specific actin network configurations contribute to the coupling of physical and physiological factors during guard cell closure dynamics, and our work presents the Arp2/3 complex and its regulators as candidate molecular players in this process.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, stabilisation of filamentous actin using phalloidin prevents ABA‐induced stomatal closure, indicating an important function of actin reorganisation in stomatal regulation (Kim et al ., 1995). Further insight into the regulatory role of actin dynamics in stomatal movement was provided by patch‐clamp analysis of stretch‐activated calcium‐permeable channels in the plasma membrane of Vicia faba guard cells (Zhang et al ., 2007). It was shown that actin disassembly facilitates the stretch activation of these channels, and subsequently mediates elevation of cytoplasmic Ca 2+ concentration (Zhang et al ., 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Further insight into the regulatory role of actin dynamics in stomatal movement was provided by patch‐clamp analysis of stretch‐activated calcium‐permeable channels in the plasma membrane of Vicia faba guard cells (Zhang et al ., 2007). It was shown that actin disassembly facilitates the stretch activation of these channels, and subsequently mediates elevation of cytoplasmic Ca 2+ concentration (Zhang et al ., 2007). Interestingly, it seems that apoplastic Ca 2+ influx plays an indispensable role during stomatal response to darkness (Schwartz et al ., 1988).…”

Section: Introductionmentioning

confidence: 99%

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Actin filament reorganisation controlled by the SCAR/WAVE complex mediates stomatal response to darkness

Isner

Costa

et al. 2017

New Phytologist

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Summary Stomata respond to darkness by closing to prevent excessive water loss during the night. Although the reorganisation of actin filaments during stomatal closure is documented, the underlying mechanisms responsible for dark‐induced cytoskeletal arrangement remain largely unknown.We used genetic, physiological and cell biological approaches to show that reorganisation of the actin cytoskeleton is required for dark‐induced stomatal closure.The opal5 mutant does not close in response to darkness but exhibits wild‐type (WT) behaviour when exposed to abscisic acid (ABA) or CaCl2. The mutation was mapped to At5g18410, encoding the PIR/SRA1/KLK subunit of the Arabidopsis SCAR/WAVE complex. Stomata of an independent allele of the PIR gene (Atpir‐1) showed reduced sensitivity to darkness and F1 progenies of the cross between opal5 and Atpir‐1 displayed distorted leaf trichomes, suggesting that the two mutants are allelic. Darkness induced changes in the extent of actin filament bundling in WT. These were abolished in opal5. Disruption of filamentous actin using latrunculin B or cytochalasin D restored wild‐type stomatal sensitivity to darkness in opal5.Our findings suggest that the stomatal response to darkness is mediated by reorganisation of guard cell actin filaments, a process that is finely tuned by the conserved SCAR/WAVE–Arp2/3 actin regulatory module.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Actin filament reorganisation controlled by the SCAR/WAVE complex mediates stomatal response to darkness

Isner

Costa

et al. 2017

New Phytologist

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“…So, different soil water supply will result in quite different physiological pathways, which directly determine the ability for plants to make photosynthetic products. Water deficits in soil environment also influence solute transport (ion and nutrient uptake of plants) to larger extent, which effects on photosynthetic reactions in plant chloroplasts in many ways [117,120,131,135,[139][140][141][142][143][144][145][146][147]. This is the reason that ion homeostasis and redox state have been brought to attention [4][5][6][7][21][22][23][24][25][26][27][28][29][111][112][113][114][115][116][117][118][119].…”

Section: Physiological Theories: Understanding Higher Plant Physiologmentioning

confidence: 99%

“…In the wide field of plant biology, its core is the study for life activities at molecular level, whose interactions at various soil-root biointerfaces at different scales are quite important to keep a steady state between plants and changing environment, especially adverse surroundings. In practice, the key point to agriculture is how to regulate the harmonious relationship between soil-environment and crops and make the best of physiological potential of crops [137][138][139][140][141][142][143][144][145][146][147]. So, adaptation in plants is an important topic in basic and applied biology under global climate change [11,[15][16][17][18][19][43][44][45][46][47][48][104][105][106][107]120].…”

Section: Introductionmentioning

confidence: 99%

The mutual responses of higher plants to environment: Physiological and microbiological aspects

Zhen-Kuan

Wang

et al. 2007

Colloids and Surfaces B: Biointerfaces

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Guard Cell Actin Cytoskeleton

Jiang

Shimono

Day

2019

Encyclopedia of Life Sciences

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Stomata are adjustable pores found predominantly in the leaf epidermis that control CO 2 influx into the apoplast and transpirational water loss. Guard cells enclosing the stomatal pore can perceive and respond to diverse stimuli via a complex signalling network: central to this signalling network is the actin cytoskeleton, which has been proposed as a critical component of stomatal guard cell function. Indeed, guard cell actin cytoskeletal filaments undergo dramatic conformational changes during biotic and abiotic stimulus‐induced stomatal movement. As a function of the genetic requirement for these highly regulated conformational changes, disruption of actin filament dynamics using pharmacological or genetic‐based approaches leads to stomatal insensitivity to the opening and closing signals. Recent advances in high‐resolution live‐cell microscopy have enabled the visualisation of the dynamic features of individual actin filaments, and in total, provided new knowledge about the quantitative properties of filament distribution and turnover. Additional studies have also revealed a spectrum of actin regulatory components and stomatal signalling events that are associated with cytoskeletal actin function in guard cells. Future research on stomatal actin signalling mutants will shed light on the mechanisms underlying the regulation of cell physiological processes and activation of responses to external stresses via actin re‐modelling. Key Concepts The guard cell actin cytoskeleton serves as a hub of the stomatal signalling network. The cortical actin arrays in guard cells rearrange rapidly to facilitate stomatal movement induced by biotic and abiotic stimuli. Reorganisation of actin filaments in guard cells is orchestrated by a diverse of actin‐binding proteins. The activation and repression of actin‐binding proteins are coordinated by a multitude of early signalling components of the stomatal signalling network. The reorganisation of cytoskeletal actin is mechanistically coupled to key guard cell signalling events such as transmembrane ion flux, membrane recycling and morphological changes of vacuoles.

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Osmo-Sensitive and Stretch-Activated Calcium-Permeable Channels in Vicia faba Guard Cells Are Regulated by Actin Dynamics

Cited by 84 publications

References 58 publications

Actin filament reorganisation controlled by the SCAR/WAVE complex mediates stomatal response to darkness

Actin filament reorganisation controlled by the SCAR/WAVE complex mediates stomatal response to darkness

The mutual responses of higher plants to environment: Physiological and microbiological aspects

Guard Cell Actin Cytoskeleton

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