Osmometry with single Sephadex beads

Ogston, A. G.; Wells, John D.

doi:10.1042/bj1190067

Cited by 32 publications

(11 citation statements)

References 9 publications

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“…Earlier investigators also analyzed the influence of smoking, alcohol consumption and oral contraceptives intake. Fibrinogen levels were found to be significantly higher in male smokers than non-smokers (Howell, 1970 andOgston, 1970). ( Williamand phillips2000) .biochemical parameters we see in the results in Table, (2) showed that there were a significant increase serum estrogen in patients of breast cancer women compared to the control group.…”

Section: Discussionmentioning

confidence: 92%

Study effect of Breast Cancer on Some Hematological and Biochemical Parameters in Babylon Province, Iraq

Ali¹

2014

IOSRJPBS

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This is a cross sectional study of variations of Hematological and biochemical parameters among female breast cancer patients and apparently healthy individuals in Babylon province 30 breast cancer patients from daily clinic and private hospitals and 20 control individual were studied between January and June, 2013. Questionnaires for bio data administered to both the patients (cases) and control subjects. Hematological parameters were run on the EDTA samples for packed cell volume (PCV), white blood cell count (WBCs),red blood cell count (RBCs), platelet count, lymphocytes count using(Auto-Hematology Analyzer-, Hungary2011). Erythrocyte sedimentation rate (ESR) also performed on the sodium citrate samples using the Western green method. Significant differences (p < 0.05) were seen in the values for WBCs, RBCs, platelets count, PCV, lymphocyte and ESR (p >0.05) among patients.The mean PCV, RBCs and WBCs,platelet count, lymphocyte value were lower in patients compared to the controls with statistically significant differences (p<0.05). However, the mean ESR value significantly higher than the control subjects (p < 0.05). This study has shown that anemia; leucopenia and thrombocytopenia were common basic features to be considered in breast cancer patients while the biochemical parameters included the level of estrogen, progesterone hormones, cholesterol, triglycerides, lipoproteins (HDL, LDL), some immunoglobulin's (IgG, IgA and IgM) also had been measured in the study. The results demonstrated significantly high values of estrogen, progesterone hormones, triglyceride, LDL, HDL, cholesterol, and significantly high values in immunoglobulin's IgA, IgG,and IgM.in breast cancer in comparison with control group,

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Section: Discussionmentioning

confidence: 92%

Study effect of Breast Cancer on Some Hematological and Biochemical Parameters in Babylon Province, Iraq

Ali¹

2014

IOSRJPBS

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“…1 presents results obtained with three different polysaccharides studied on the larger column. Since each of these solutes is sufficiently large to be essentially excluded from the gel phase in the presence of salt Ogston & Wells, 1970;Wells, 1972), the variation in elution volume is a direct measure of changes in the void volume. In turn, such changes must arise as a consequence of shrinkage of gel beads and it is therefore relevant to examine the results in terms of eqn.…”

Section: Methodsmentioning

confidence: 99%

“…In such quantitative studies, the methods employed to allow for the effects of concentration dependence are entirely analogous to those used to correct for frictional effects in sedimentation velocity. However, the effect in chromatography is likely to be osmotic in origin Nichol et al, 1969;Ogston & Wells, 1970). The present work explores this postulate further.…”

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confidence: 99%

The origin and consequences of concentration dependence in gel chromatography

Nichol

Janado

Winzor

1973

Biochemical Journal

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Concentration dependence of elution volume was determined for Blue Dextran 2000, Dextran 500, Dextran sulphate 500 and bovine serum albumin on columns of Sephadex G-100 equilibrated with sodium phosphate buffer, I 0.1, pH6.8. From the results for Dextran 500, it was shown that a linear relation exists between elution volume and the corresponding osmotic pressure calculated for the same concentration and incorporating the term containing the second virial coefficient. This relationship was used to predict the concentration dependence of elution volume for bovine serum albumin and myoglobin, proteins that partially penetrate Sephadex G-100. Possible consequences of osmotic effects are considered in relation to various types of column experiments, including differential chromatography.

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“…Dextran 500 was from Pharmacia, batch T 5406. Solutions were made and their concentrations measured as described by Ogston & Wells (1970). Titration of solutions (see below) showed a very low content of Cl-ion.…”

Section: Experimental Materialsmentioning

confidence: 99%

The osmotic properties of sulphoethyl-Sephadex. A model for cartilage

Ogston

Wells

1972

Biochemical Journal

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1. Observations are reported on the variation of the swelling of sulphoethyl-Sephadex C-50 and C-25, and of the partition of NaCl between solution and gel, with the concentration of NaCl. 2. The results were interpreted in terms of Manning's (1969) treatment of the interactions between polyionic polymers and simple electrolytes and of Flory's (1953) treatment of the swelling of gels. 3. The results indicate net inner osmotic pressures as high as 3.6x10(5)Pa (140 mosmolar=3.6x10(5)N/m(2)) in 0.15m-NaCl. 4. It is suggested that cartilage may have net inner osmotic pressure of the order of 10(5)Pa.

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Osmometry with single Sephadex beads

Cited by 32 publications

References 9 publications

Study effect of Breast Cancer on Some Hematological and Biochemical Parameters in Babylon Province, Iraq

Study effect of Breast Cancer on Some Hematological and Biochemical Parameters in Babylon Province, Iraq

The origin and consequences of concentration dependence in gel chromatography

The osmotic properties of sulphoethyl-Sephadex. A model for cartilage

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