Osmosensitive transcription factors in the prolactin cell of a euryhaline teleost

Malintha, G H T; Celino-Brady, Fritzie T.; Stoytcheva, Zoia; Seale, André P.

doi:10.1016/j.cbpa.2022.111356

Cited by 2 publications

(6 citation statements)

References 70 publications

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“…In the tilapia RPD, pou1f1 and pou2f1b were the most highly expressed transcripts of Pit and Oct1, respectively 29 . Moreover, pou1f1 expression was inversely related with osmolality in SWacclimated tilapia 37 . Both pou1f1 and pou2f1b were inhibited by a rise in temperature.…”

Section: Discussionmentioning

confidence: 92%

“…In FW-acclimated tilapia, prl mRNA did not show any osmosensitivity, consistent with previous studies 17, 59 and the notion that prl mRNA levels in FW-sh may be at or near the maximum transcriptional activity and therby unresponsive to further osmotic stimulation 26 . On the other hand, Prl cells from SW-acclimated tilapia contain low levels of Prls, and therefore, activate prl 177 and prl 188 transcription in hyposmotic conditions 26,37,59 .…”

Section: Discussionmentioning

confidence: 99%

“…The transduction of osmotic stimuli into the activation of prl transcription is largely regulated by the activity of TFs and TF modules (TFMs) that operate in the promoter regions of prl 188 and prl 177 genes 29,37 . In Prl cells of tilapia acclimated to SW, expression of TF transcripts was more sensitive to both thermal and osmotic stimuli compared with those in FW.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, several putative transcription factors (TF) predicted to bind promoter regions of prl 177 and prl 188 were identi ed 29 and their activities measured in the tilapia Prl cell model 37 . Among them, several POU family TFs, such as pituitary transcription factor 1 (Pit1, also known as Pou1f1), a key regulator of pituitary cell differentiation 38 , were directly responsive to changes in extracellular osmolality 37 . Pit1 shares a common binding site on the tilapia prl 188 promoter region with Octamer 1 (Oct1, also known as Pou2f1), another POU family TF 29 activated by stressors [39][40][41] .…”

Section: Introductionmentioning

confidence: 99%

“…Pit1 shares a common binding site on the tilapia prl 188 promoter region with Octamer 1 (Oct1, also known as Pou2f1), another POU family TF 29 activated by stressors [39][40][41] . The roles of cAMP and Ca 2+ second messenger systems in cellular signaling have been studied, including downstream activation of CAAT/enhancer binding protein (CEBP) and cAMP response element binding protein (CREB) 42,43 , two TFs also predicted to bind prl 188 and prl 177 promoter regions 29 and recently shown to be hyposmotically induced in tilapia Prl cells 37 . On the other hand, the nuclear factor of activated T cells (NFAT) is hyperosmosensitive, leading to the production of secondary metabolites 44,45 ; binding sites for NFAT were found in the promoter region of tilapia prl 177 , but not prl 188 29 .…”

Section: Introductionmentioning

confidence: 99%

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Temperature modulates the osmosensitivity of tilapia prolactin cells

Hewage

Woo

Celino-Brady

et al. 2023

Preprint

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In euryhaline fish, prolactin (Prl) plays an essential role in freshwater (FW) acclimation. In the euryhaline and eurythermal Mozambique tilapia, Oreochromis mossambicus, Prl cells are model osmoreceptors, recently described to be thermosensitive. To investigate the effects of temperature on osmoreception, we incubated Prl cells of tilapia acclimated to either FW or seawater (SW) in different temperature (20, 26 and 32°C) and osmolality (280, 330 and 420 mOsm/kg) combinations for 6 h. Release of both Prl isoforms, Prl188 and Prl177, increased in hyposmotic media and were further augmented with a rise in temperature. Hyposmotically-induced release of Prl188 was inhibited at 20°C. In SW fish, mRNA expression of prl188 and prl177 showed direct and inverse relationships with temperature, respectively. In SW-acclimated tilapia Prl cells incubated in hyperosmotic media, Prl receptors, prlr1 and prlr2, and the stretch-activated Ca2+ channel, trpv4,were inhibited at 32°C, suggesting the presence of a cellular mechanism to compensate for elevated Prl release. Transcription factors, pou1f1, pou2f1b, creb3l1, cebpb, stat3, stat1a and nfat1c, known to regulate prl188 and prl177, were also downregulated at 32°C. Our findings provide evidence that osmoreception is modulated by temperature, and that both thermal and osmotic responses vary with acclimation salinity.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Temperature modulates the osmosensitivity of tilapia prolactin cells

Hewage

Woo

Celino-Brady

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Temperature modulates the osmosensitivity of tilapia prolactin cells

Malintha,

Woo,

Celino-Brady

et al. 2023

Sci Rep

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In euryhaline fish, prolactin (Prl) plays an essential role in freshwater (FW) acclimation. In the euryhaline and eurythermal Mozambique tilapia, Oreochromis mossambicus, Prl cells are model osmoreceptors, recently described to be thermosensitive. To investigate the effects of temperature on osmoreception, we incubated Prl cells of tilapia acclimated to either FW or seawater (SW) in different combinations of temperatures (20, 26 and 32 °C) and osmolalities (280, 330 and 420 mOsm/kg) for 6 h. Release of both Prl isoforms, Prl188 and Prl177, increased in hyposmotic media and were further augmented with a rise in temperature. Hyposmotically-induced release of Prl188, but not Prl177, was suppressed at 20 °C. In SW fish, mRNA expression of prl188 increased with rising temperatures at lower osmolalities, while and prl177 decreased at 32 °C and higher osmolalities. In Prl cells of SW-acclimated tilapia incubated in hyperosmotic media, the expressions of Prl receptors, prlr1 and prlr2, and the stretch-activated Ca2+ channel, trpv4,decreased at 32 °C, suggesting the presence of a cellular mechanism to compensate for elevated Prl release. Transcription factors, pou1f1, pou2f1b, creb3l1, cebpb, stat3, stat1a and nfat1c, known to regulate prl188 and prl177, were also downregulated at 32 °C. Our findings provide evidence that osmoreception is modulated by temperature, and that both thermal and osmotic responses vary with acclimation salinity.

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Osmosensitive transcription factors in the prolactin cell of a euryhaline teleost

Cited by 2 publications

References 70 publications

Temperature modulates the osmosensitivity of tilapia prolactin cells

Temperature modulates the osmosensitivity of tilapia prolactin cells

Temperature modulates the osmosensitivity of tilapia prolactin cells

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