Osmotic and ionic regulation and Na+/K+-ATPase, carbonic anhydrase activities in mature Chinese mitten crab, Eriocheir sinensis H. Milne Edwards, 1853 (Decapoda, Brachyura) exposed to different salinities

Wang, Ruifang; Jia, Xiaoyan; Zhang, Longzhen; Xiao-rong, Huang; Feng, Guangpeng; Zhuang, Ping

doi:10.1163/15685403-00003125

Cited by 29 publications

(19 citation statements)

References 45 publications

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“…In agreement with these studies, the present study showed higher digestive enzyme activity as the crabs were maintained in freshwater or acclimated to lower salinities. Our study has demonstrated that mature E. sinensis strongly hyperregulated their hemolymph osmolality at a salinity range of 0-21 ppt (Table 3), and the isosmotic point approaching 28 ppt (Wang et al 2012). Therefore, higher digestive enzyme activity in the relatively low salinity environment was a profitable response of the animal in an attempt to derive as much energy as possible from the food to meet its energy requirements for hyperregulation.…”

Section: Discussionmentioning

confidence: 64%

The response of digestive enzyme activity in the mature Chinese mitten crab, <i>Eriocheir sinensis</i> (Decapoda: Brachyura), to gradual increase of salinity

et al. 2013

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SUMMARY: Mature Chinese mitten crabs, Eriocheir sinensis, were exposed to brackish water or seawater as an obligatory part of their reproductive migration. Physiological and biochemical reorganization were needed to adapt them to this migration. To understand the digestive adjustments of Eriocheir sinensis at biochemical level during this transformation from freshwater to seawater, the response of the activity of five digestive enzymes (amylase, cellulase, pepsin, trypsin and lipase) in the hepatopancreas to salinities increasing gradually from 0 (freshwater) to 35 (seawater) was analysed in mature females and males. Digestive enzymes exhibited significantly higher activities in the hepatopancreas of males than those of females, except lipase. In females, amylase, pepsin and trypsin activities began to decrease significantly as the salinity reached 28, and cellulase activity decreased at 35; in males, a considerable decrease in the activity of digestive enzymes, except lipase, was observed at 21 and higher salinities, while an increase was observed at 14. Reduced enzyme activities at elevated salinities suggest that the digestive capacity of crabs for diets becomes weak, and all these digestive enzymes participated in digestive adjustments during osmoregulation. The initial salinity which induced the decrease of enzyme activity was lower in males than in females, indicating that females were more tolerant to elevated salinities than males from the point of digestive biochemical modulation.Keywords: mature crab, digestive enzyme, sexual difference, metabolic regulation, osmoregulation, salinity. RESUMEN: Respuesta de la actividad enzimática digestiva al incRemento gRadual de la salinidad en el cangRejo de shanghai maduRo, EriochEir sinEnsis (decapoda: BRachyuRa). -Se expusieron cangrejos de Shanghai maduros (Eriocheir sinensis) a agua salobre o agua marina obligatoriamente durante la migración reproductora. A fin de que los ejemplares se adaptaran a esta migración, fue preciso proceder a una reorganización fisiológica y bioquímica. Con objeto de estudiar los ajustes digestivos de carácter bioquímico del Eriocheir sinensis durante la transformación del agua dulce en agua marina, se analizó la reacción de la actividad en el hepatopáncreas de cinco enzimas digestivas (amilasa, celulasa, pepsina, tripsina y lipasa) a un aumento gradual de la salinidad, desde 0 ppt (agua dulce) hasta 35 ppt (agua marina), en ejemplares maduros machos y hembras. Las enzimas digestivas mostraron un grado de actividad notablemente mayor en el hepatopán-creas de los machos que en el de las hembras, con excepción de la lipasa. En las hembras, la actividad de la amilasa, la pepsina y la tripsina comenzó a reducirse notablemente cuando la salinidad alcanzó las 28 ppt, mientras que la actividad de la celulasa descendió cuando se alcanzaron las 35 ppt; en el caso de los machos, se observó un descenso muy pronunciado de la actividad enzimática digestiva a partir de las 21 ppt, aunque la actividad aumentó a las 14 ppt. La menor actividad enzimát...

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Section: Discussionmentioning

confidence: 64%

The response of digestive enzyme activity in the mature Chinese mitten crab, <i>Eriocheir sinensis</i> (Decapoda: Brachyura), to gradual increase of salinity

et al. 2013

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“…Superoxide dismutase (SOD) is an important antioxidant enzyme that can scavenge free radicals and prevents tissue damages in crustaceans (Li et al, 2008 ; Lu et al, 2011 ). The 6‰ salinity resulted in the lowest hemolymph SOD activity compared to other salinities, suggesting relatively lower level of free radicals in hemolymph of the female E. sinensis under the salinity (Chien et al, 2003 ; Wang et al, 2012 ). Meanwhile, malondialdehyde (MDA) is an indicator of lipid peroxidation, its content often reflects the levels of free radicals and cellular oxidation in tissues (Long et al, 2017a ), both hemolymph and hepatopancrea MDA of the crabs were the lowest at 6‰ as compared to other salinities, again indicating lower peroxide stress in tissues at the salinity.…”

Section: Discussionmentioning

confidence: 99%

“…E. sinensis is known as a euryhaline species and a strong osmoregulator (Rathmayer and Siebers, 2001 ; Wang et al, 2012 ). It is well-documented that various ions and free amino acids are the main contributors to hemolymph osmolality of crustaceans; as facilitated by various ion transport enzymes and proteins, they play crucial roles in crustacean osmoregulation (Towle and Weihrauch, 2001 ; Romano and Zeng, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Physiological Responses and Ovarian Development of Female Chinese Mitten Crab Eriocheir sinensis Subjected to Different Salinity Conditions

Long

Zhao

et al. 2018

Front. Physiol.

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Salinity plays a key role affecting ovarian development, osmoregulation and metabolism of female Chinese mitten crab, Eriocheir sinensis during reproductive migration. In this study, female E. sinensis after their puberty molt were subjected to four salinities of 0, 6, 12, and 18‰ for 40 days to investigate the salinity effects on their ovarian development as well as a range of important physiological parameters. Elevated salinity accelerated the ovarian development with ovigerous crabs found at salinity treatments of 12 and 18‰ despite no copulation had occurred. Meanwhile the survival rate of female crabs showed a decreasing trend with increasing salinity. Higher salinity also led to increased hemolymph Na+, K+, Ca2+, Cl−, and Mg2+ concentrations. The 6‰ treatment had the highest contents of hemolymph total and major free amino acids while the Na+/K+ -ATPase activity in the posterior gills was the lowest among treatments. Total n-3 polyunsaturated fatty acids (∑n-3PUFA) and n-3/n-6 PUFA ratio in the anterior gills showed a decreasing trend with salinity while 18‰ had the highest ∑PUFA and ∑n-6PUFA. The ∑n-3PUFA content and n-3/n-6 PUFA ratio of the posterior gills showed a fluctuating pattern and the highest value was detected at 0‰, while an increasing trend was found for the ∑n-6PUFA with increasing salinity. The hemolymph glucose showed a decreasing trend with increasing salinity and the highest total cholesterol in hemolymph was detected at 12‰. The 18‰ treatment had the highest levels of hemolymph γ-glutamyltransferase, alkaline phosphatase and acid phosphatase, as well as glucose, urea and acid phosphatase in hepatopancreas while the highest hemolymph superoxide dismutase and malondialdehyde were detected at 0‰. Overall, the results showed that salinity increase from freshwater to brackish conditions led to lower metabolism, accelerated ovarian development, and the appearance of ovigerous crabs without copulation in female E. sinensis post puberty molt.

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“…E . sinensis is a euryhaline species and a strong osmoregulator [5, 6]. During reproductive and growth migrations, E .…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis reveals osmotic-regulated genes in the gill of Chinese mitten crab (Eriocheir sinensis)

et al. 2019

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Salinity is one of the most important abiotic factors directly affecting the reproduction, molting, growth, immune, physiological and metabolic activities of Chinese mitten crab (Eriocheir sinensis). This species has strong osmoregulatory capacity and can maintain stringent internal homeostasis. However, the mechanisms conferring tolerance to salinity fluctuations are not well understood. To reveal the genes and pathways involved in osmoregulation, adult male crabs (body weight = 110 ± 5 g) were acclimated for 144 h in freshwater (FW, 0 ppt) or seawater (SW, 25 ppt). Changes in the transcriptome of crab gills were then analysed by RNA-Seq, and 174,903 unigenes were obtained. Comparison of genes between FW- SW-acclimated groups identified 932 genes that were significantly differentially expressed in the gill, comprising 433 and 499 up- and downregulated transcripts. Gene Ontology functional enrichment analysis revealed that important biological processes related to salt stress were significantly enriched, including energy metabolism, ion transport, signal transduction and antioxidant activity. Kyoto Encyclopaedia of Genes and Genomes enrichment analysis mapped the differentially expressed genes to 241 specific metabolic pathways, and pathways related to energy metabolism, oxidative phosphorylation and the tricarboxylic acid (TCA)/citrate cycle were significantly enriched. Salinity stress altered the expression of many enzymes involved in energy metabolism, ion transport, signal transduction and antioxidant pathways, including citrate synthase (CS), Na+/K+-ATPase (NKA), Na+-K+-2Cl cotransporter-1 (NKCC1), dopamine receptor D1 (DRD1), synaptic binding protein 1 (STXBP1), Cu2+/Zn2+ superoxide dismutase (SOD1) and glutathione S-transferase (GST). Additionally, the obtained transcriptomic sequencing data provided a useful resource for identification of novel genes, and further physiological analysis of Chinese mitten crab.

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Osmotic and ionic regulation and Na+/K+-ATPase, carbonic anhydrase activities in mature Chinese mitten crab, Eriocheir sinensis H. Milne Edwards, 1853 (Decapoda, Brachyura) exposed to different salinities

Cited by 29 publications

References 45 publications

The response of digestive enzyme activity in the mature Chinese mitten crab, <i>Eriocheir sinensis</i> (Decapoda: Brachyura), to gradual increase of salinity

The response of digestive enzyme activity in the mature Chinese mitten crab, <i>Eriocheir sinensis</i> (Decapoda: Brachyura), to gradual increase of salinity

Physiological Responses and Ovarian Development of Female Chinese Mitten Crab Eriocheir sinensis Subjected to Different Salinity Conditions

Comparative transcriptome analysis reveals osmotic-regulated genes in the gill of Chinese mitten crab (Eriocheir sinensis)

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