Osmotic and solute permeabilities of isolated urinary bladder of the trout

Fossat, B.; Lahlou, B.

doi:10.1152/ajprenal.1977.233.6.f525

Cited by 8 publications

(9 citation statements)

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“…They also agree with data obtained from other teleostean species (Hogben, Brandes, Danforth & Forster, 1972;Renfro, 1975Renfro, , 1977 (Fossat & Lahlou, 1977;Renfro, 1977). The current experiments demonstrate that the coupling is obligatory and specific and occurs at the luminal side of the cells.…”

Section: Discussionsupporting

confidence: 92%

“…Under these conditions the fish bladder actively transports Na+ and Clin equal amounts from the mucosal to the serosal side. Interdependence of Na and Cl transport was shown from ionic substitution experiments (Fossat & Lahlou, 1977;Renfro, 1977). The current experiments demonstrate that the coupling is obligatory and specific and occurs at the luminal side of the cells.…”

Section: Discussionsupporting

confidence: 54%

“…Radioactivity procedures Sodium fluxes were determined by using 24NaCl obtained from the Commissariat 'a l'Energie Atomique, Saclay (F) In the uptake experiments, correction for the volume of the adherent radioactive solution was made by using [3H]inulin which is a reliable extracellular marker for fish urinary bladder. Radioactive mannitol, which is currently used for other epithelia in similar experiments, was considered unsuitable because its permeability coefficient is similar to that of Na in trout bladder (Fossat & Lahlou, 1977).…”

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confidence: 99%

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The mechanism of coupled transport of sodium and chloride in isolated urinary bladder of the trout.

Fossat¹,

Lahlou²

1979

The Journal of Physiology

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SUMMARY1. Transepithelial fluxes of Na and Cl were measured in dissected trout bladders mounted in Ussing chambers. The mucosa-to-serosa fluxes appeared to comprise a linear and a saturating (Km = 8 mM) component as a function of the luminal concentration of the ion. The over-all permeability for Cl was higher than for Na. Removal of either ion from the mucosal side induced the disappearance of the net flux of the co-ion even when the substituting ions were not impermeant. This indicated a complete and specific mutual dependence between the Na and Cl transports. A large part (30-500 %) of Cl exchanges was ascribed to an exchangediffusion process.2. In order to localize the site of ion coupling, the apical uptakes of Na and Cl were measured by short-term exposure (2 min) to radioisotopes. The apical fluxes were similar to the transepithelial fluxes and therefore appeared to be the limiting factor for ion transfer. Ionic replacements on the mucosal side showed that the coupling between Na and Cl and Cl/Cl exchange occurs at this step.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 54%

mentioning

confidence: 99%

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The mechanism of coupled transport of sodium and chloride in isolated urinary bladder of the trout.

Fossat¹,

Lahlou²

1979

The Journal of Physiology

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“…When it is present, it is the site of active reabsorption of both Na + and CC with water following osmotically (Hirano et al, 1973, Renfro, 1975, Fossat and Lahlou, 1977. In freshwater teleosts it appears that the bladder reabsorbs a urinehyperosmotic fluid due to a rather low osmotic permeability (Hirano et al, 1973).…”

Section: Freshwater Teleostsmentioning

confidence: 99%

Osmotic and Ionic Regulation by Freshwater and Marine Fishes

Evans

1980

Environmental Physiology of Fishes

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“…A serosa positive change in E; may occur following amphotericin B given the hypertonic nature of NaCl transport (Fossat & Lahlou, 1977) and the apparent anion selectivity of the shunt pathway (Fossat & Lahlou, 1979a). However, we calculate that little change in Ri occurs and furthermore transepithelial Cl-fluxes are not modified by amphotericin B (Fossat & Lahlou, 1982 (Fossat & Lahlou, 1982).…”

Section: Effects Of Amphotericin B On Electrical Parametersmentioning

confidence: 82%

Ion‐selective micro‐electrode studies of the electrochemical potentials in trout urinary bladder.

Harvey¹,

Lahlou²

1986

The Journal of Physiology

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SUMMARY1. Intracellular micro-electtode techniques were used to measure the electrical resistances of the cell membranes and the shunt pathway and intracellular ionic activities in trout urinary bladder when the tissue was incubated in Ringer solution and in the presence of the polyene antibiotic ionophore amphotericin B.2. In control conditions the transepithelial potential was zero and the intracellular potential was -56 mV. The intracellular ionic activities measured with singleand double-barrel ion-sensitive micro-electrodes for the first time in a fish bladder (aka = 16 mm, ak = 87 mm, and a'1 = 21 mM) indicate an active accumulation of K and Cl ions and an active extrusion of Na ions by the cell. The maintenance of intracellular Cl activity above its equilibrium value depended on the presence of Na ions in the mucosal medium, but was independent of the presence of K ions.3. Flat cable analysis yielded values for transepithelial, apical, basolateral and shunt resistances of 197, 2790, 1986 and 205 1 cm-2 respectively. Equivalent circuit analysis using amphotericin B yielded similar values for shunt resistance. The paracellular pathway accounts for 96% of transepithelial current flow and this epithelium may be classified as 'leaky'. The cells are electrically coupled with a space constant of 354 sm.4. Amphotericin B when added to the mucosal solution induced an immediate serosa positive transepithelial potential of about 9 mV and a short-circuit current of 64 1zA cm-2. The Vt was ouabain sensitive and dependent on mucosal Na concentration. The origin of the antibiotic induced transepithelial potential was an increase in the sum of the cell membrane electromotive forces. The apical membrane potential depolarized to -7 mV and its resistance fell to 433 Q cm-2. During the first 10 min ofexposure a'a increased to 80 mm and ak decreased to 7 mm with only a small change in ai1. The changes in cellular Na+ and K+ activities were in accordance with their passive redistribution down their electrochemical gradients. 468 B. J. HARVEY AND B. LAHLOU

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Osmotic and solute permeabilities of isolated urinary bladder of the trout

Cited by 8 publications

References 0 publications

The mechanism of coupled transport of sodium and chloride in isolated urinary bladder of the trout.

The mechanism of coupled transport of sodium and chloride in isolated urinary bladder of the trout.

Osmotic and Ionic Regulation by Freshwater and Marine Fishes

Ion‐selective micro‐electrode studies of the electrochemical potentials in trout urinary bladder.

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