Osmotic deformability of erythrocytes at various shear stresses

Heo, Yujin; Jung, Hyun-Woo; Shin, Sehyun

doi:10.3233/ch-131761

Cited by 10 publications

(8 citation statements)

References 39 publications

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“…EI max is usually observed at isotonic conditions during high shear stress (>20 Pa). However, a recent study [15] reported that RBC deformability at low shear stress (1-3 Pa) was maximal in hypotonic conditions (225-250 mOsm/kg H 2 O), which is slightly lower than the normal range of osmolality in plasma (290-310 mOsm/kg H 2 O). The osmolality at EI max (O max ) was found to be dependent on applied shear stress, which could play an important role in microcirculation processes such as the Fåhraeus-Lindqvist effect [16].…”

Section: Osmotic Concentrationsmentioning

confidence: 89%

Advances in the measurement of red blood cell deformability: A brief review

Kim

Lee

Shin

2015

JCB

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146

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Red blood cells (RBCs) exhibit a unique deformability, which enables them to change shape reversibly in response to an external force. The deformability of RBCs allows them to flow in microvessels while transporting oxygen and carbon dioxide. In this review, we discussed the major determinants of RBC deformability, which include cell geometry, internal viscosity, rheological properties of the membrane, osmotic pressure, calcium, nitric oxide, temperature, ageing, and depletion of adenosine triphosphate. Additionally, we highlighted the various methods and techniques used to measure RBC deformability. Individual cell analyses (pipette aspiration and optical tweezers) and bulk cell analyses (ektacytometry, multiple channels) were described and compared. Finally, we reviewed the correlation between RBC deformability and clinical outcomes such as diabetic microangiopathy.

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Section: Osmotic Concentrationsmentioning

confidence: 89%

Advances in the measurement of red blood cell deformability: A brief review

Kim

Lee

Shin

2015

JCB

Self Cite

146

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“…The least noisy readouts were obtained at 30 Pa. Choice of this supraphysiological shear stress levels for both machines had an impact of maximal deformability EImax, which was shown to increase with a growing shear stress, and on the ionic strength O_EI_max at which maximal deformability is reached (Heo et al, 2015;Nemeth et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Donor Age and Red Cell Age Contribute to the Variance in Lorrca Indices in Healthy Donors for Next Generation Ektacytometry: A Pilot Study

et al. 2021

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The ability of red blood cells (RBCs) to transport gases, their lifespan as well as their rheological properties invariably depend on the deformability, hydration, and membrane stability of these cells, which can be measured by Laser optical rotational red cell analyser (Lorrca® Maxsis, RR Mechatronics). The osmoscan mode of Lorrca is currently used in diagnosis of rare anemias in clinical laboratories. However, a broad range of normal values for healthy subjects reduces the sensitivity of this method for diagnosis of mild disease phenotype. In this pilot study, we explored the impact of age and gender of 45 healthy donors, as well as RBC age on the Lorrca indices. Whereas gender did not affect the Lorrca indices in our study, the age donors had a profound effect on the O_hyper parameter. To study the impact of RBC age on the osmoscan parameters, we have isolated low (L)-, medium (M)-, or high (H)- density fractions enriched with young, mature, and senescent RBCs, respectively, and evaluated the influence of RBC age-related properties, such as density, morphology, and redox state, on the osmoscan indices. As before, O_hyper was the most sensitive parameter, dropping markedly with an increase in RBC density and age. Senescence was associated with a decrease in deformability (EI_max) and tolerability to low and high osmolatites (Area). L-fraction was enriched with reticulocytes and cells with high projected area and EMA staining, but also contained a small number of cells small in projected area and most likely, terminally senescent. L-fraction was on average slightly less deformable than mature cells. The cells from the L-fraction produced more oxidants and NO than all other fractions. However, RBCs from the L-fraction contained maximal levels of reduced thiols compared to other fractions. Our study suggests that reference values for O_hyper should be age-stratified, and, most probably, corrected for the average RBC age. Further multi-center study is required to validate these suggestions before implementing them into clinical practice.

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“…The osmotic gradient ektacytometric measurements ( Heo et al, 2015 ; Nemeth et al, 2015 ; Huang et al, 2019 ) were obtained using the “osmoscan function” of the above optical rotational red cell analyzer device (LoRRca). The device generated a constant shear stress of 30 Pa while continuously aspirating the sample into the measurement site and while changing the osmolarity of the medium by using gradual mixtures of PVP solutions of 0 and 700 mOsmol/kg; therefore, the EI was continuously registered.…”

Section: Methodsmentioning

confidence: 99%

“…The device generated a constant shear stress of 30 Pa while continuously aspirating the sample into the measurement site and while changing the osmolarity of the medium by using gradual mixtures of PVP solutions of 0 and 700 mOsmol/kg; therefore, the EI was continuously registered. The parameters measured and calculated by the device (all at shear stress of 30 Pa) were as follows ( Heo et al, 2015 ; Nemeth et al, 2015 ; Huang et al, 2019 ):…”

Section: Methodsmentioning

confidence: 99%

Supervised Cycling Training Improves Erythrocyte Rheology in Individuals With Peripheral Arterial Disease

Hsu

Lin

et al. 2022

Front. Physiol.

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Peripheral arterial disease (PAD) results in insufficient flow to lower extremities. Aerobic exercise provides health benefits for individuals with PAD, but basic science behind it is still debated. Twenty-one PAD patients aged about 70 years with female/male as 7/14 were recruited. Among them, 11 were randomized to have supervised cycling training (SCT) and 10 to receive general healthcare (GHC) as controls. SCT participants completed 36 sessions of SCT at the first ventilation threshold within 12 weeks and the controls received GHC for 12 weeks. Ankle-brachial index (ABI), 6-min walk test (6MWT), peak oxygen consumption (V˙O2peak), minute ventilation (V˙E), minute carbon dioxide production (V˙CO2), erythrocyte rheology, including the maximal elongation index (EImax) and shear stress at 50% of maximal elongation (SS1/2), and the Short Form-36 (SF-36) questionnaire for quality of life (QoL) were assessed before and 12 weeks after initial visit. SCT significantly decreased the SS1/2 as well as SS1/2 to EImax ratio (SS1/2/EImax) and increased the erythrocyte osmolality in the hypertonic region as well as the area under EI-osmolality curve. The supervised exercise-induced improvement of erythrocyte deformability could contribute to the increased peripheral tissue O2 delivery and was possibly related with increased V˙O2peak. The physiological benefit was associated with significantly increased ABI, 6-min walking distance, cardiorespiratory fitness, and SF-36 score. However, no significant changes in aerobic capacity and erythrocyte rheological properties were observed after 12-week of GHC. In conclusion, SCT improves aerobic capacity by enhancing erythrocyte membrane deformability and consequently promotes QoL in PAD patients.

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Osmotic deformability of erythrocytes at various shear stresses

Cited by 10 publications

References 39 publications

Advances in the measurement of red blood cell deformability: A brief review

Advances in the measurement of red blood cell deformability: A brief review

Donor Age and Red Cell Age Contribute to the Variance in Lorrca Indices in Healthy Donors for Next Generation Ektacytometry: A Pilot Study

Supervised Cycling Training Improves Erythrocyte Rheology in Individuals With Peripheral Arterial Disease

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