2008
DOI: 10.1016/j.jmb.2008.05.081
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Osmotic Pressure: Resisting or Promoting DNA Ejection from Phage?

Abstract: Recent in vitro experiments have shown that DNA ejection from bacteriophage can be partially stopped by surrounding osmotic pressure when ejected DNA is digested by DNase I in the course of ejection. In this work, we argue by a combination of experimental techniques (osmotic suppression without DNase I monitored by UV absorbance, pulse-field electrophoresis, and cryo-transmission electron microscopy visualization) and simple scaling modeling that intact genome (i.e., undigested) ejection in a crowded environme… Show more

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Cited by 35 publications
(39 citation statements)
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References 74 publications
(172 reference statements)
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“…If γ ¼ 3=5, then R H ≈ 1650 nm, which would exceed the width of an E. coli cell (roughly 1 μm). For γ ¼ 1=3, R H ≈ 350 nm is smaller than the cell size so, hereafter, we will assume that the phage DNA in the cell is a compacted blob and set γ ¼ 1=3, consistent with previous experimental evidence that the injected phage DNA remains near the site of entry in the collapsed state [34,36,51]. As ejection progresses, DNA newly released into the cell is expected to collapse onto the blob.…”
Section: Diffusive Regimementioning
confidence: 95%
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“…If γ ¼ 3=5, then R H ≈ 1650 nm, which would exceed the width of an E. coli cell (roughly 1 μm). For γ ¼ 1=3, R H ≈ 350 nm is smaller than the cell size so, hereafter, we will assume that the phage DNA in the cell is a compacted blob and set γ ¼ 1=3, consistent with previous experimental evidence that the injected phage DNA remains near the site of entry in the collapsed state [34,36,51]. As ejection progresses, DNA newly released into the cell is expected to collapse onto the blob.…”
Section: Diffusive Regimementioning
confidence: 95%
“…Another passive mechanism favoring ejection proposed in Ref. [36] is due to phage DNA condensation in a crowded environment and solvent exclusion generating an effective pulling force. However, if that were the dominating mechanism, the ejection force would grow with the amount of ejected DNA, inconsistent with the observed in vivo ejection speed, which decreases as ejection proceeds [ Fig.…”
Section: Introductionmentioning
confidence: 93%
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“…Une des clés pour une meilleure compréhension de ce phénomène pourrait être l'organisation adoptée par l'ADN dans les conditions extrêmes régnant dans la capside. À cette densité, l'ADN est un cristal liquide, avec un ordre hexagonal mis en évidence dès les années 60 par diffraction des rayons X. Mais, au-delà de cet ordre local, le repliement de la chaîne, qui est unique à chaque phage, est mal compris, et beaucoup reste à faire pour comprendre l'intrication entre empaquetage et éjection, pression et organisation de l'ADN [4,5].…”
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