Osmotic regulation of neuronal nitric oxide synthase expression in the rat amygdala: Functional role for nitric oxide in adaptive responses?

Yao, Song T.; Antunes, Vagner Roberto; Paton, Julian F. R.; Murphy, David

doi:10.1002/jnr.21114

Cited by 7 publications

(6 citation statements)

References 36 publications

(60 reference statements)

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“…At the end of the plus maze test, animals were allowed to drink water freely, and the respective intakes at 30 and 120 minutes (Figures 7A, B and Supplementary Table 6) were recorded. As expected, water-deprived rats drank more water than the control animals at 30 and at 120 minutes [F (1,33) =504.8; p<0.0001 and F (1,33) =521.4; p<0.0001; respectively]. However, the oxytocin receptor antagonist had no effect on the water drank by the rats (Figures 7A, B).…”

Section: Effect Of Oxtr Antagonist Microinjection In Ceasupporting

confidence: 67%

Water deprivation induces hypoactivity in rats independently of oxytocin receptor signaling at the central amygdala

Felintro

Trujillo²,

Dos‐Santos³

et al. 2023

Front. Endocrinol.

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IntroductionVasopressin (AVP) and oxytocin (OXT) are neuropeptides produced by magnocellular neurons (MCNs) of the hypothalamus and secreted through neurohypophysis to defend mammals against dehydration. It was recently demonstrated that MCNs also project to limbic structures, modulating several behavioral responses.Methods and ResultsWe found that 24 h of water deprivation (WD) or salt loading (SL) did not change exploration or anxiety-like behaviors in the elevated plus maze (EPM) test. However, rats deprived of water for 48 h showed reduced exploration of open field and the closed arms of EPM, indicating hypoactivity during night time. We evaluated mRNA expression of glutamate decarboxylase 1 (Gad1), vesicular glutamate transporter 2 (Slc17a6), AVP (Avpr1a) and OXT (Oxtr) receptors in the lateral habenula (LHb), basolateral (BLA) and central (CeA) amygdala after 48 h of WD or SL. WD, but not SL, increased Oxtr mRNA expression in the CeA. Bilateral pharmacological inhibition of OXTR function in the CeA with the OXTR antagonist L-371,257 was performed to evaluate its possible role in regulating the EPM exploration or water intake induced by WD. The blockade of OXTR in the CeA did not reverse the hypoactivity response in the EPM, nor did it change water intake induced in 48-h water-deprived rats.DiscussionWe found that WD modulates exploratory activity in rats, but this response is not mediated by oxytocin receptor signaling to the CeA, despite the upregulated Oxtr mRNA expression in that structure after WD for 48 h.

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Section: Effect Of Oxtr Antagonist Microinjection In Ceasupporting

confidence: 67%

Water deprivation induces hypoactivity in rats independently of oxytocin receptor signaling at the central amygdala

Felintro

Trujillo²,

Dos‐Santos³

et al. 2023

Front. Endocrinol.

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“…The reasons for this difference are presently unknown. Water deprivation represents a globally stressful experience for individuals, and BLA is an important region for stress responses [57][58][59]. Therefore, it is tempting to speculate that the cortical control of BLA activity might mitigate the stress component induced by water deprivation.…”

Section: Acc Neurons Projecting To Bla: a Novel Locus For Control Of ...mentioning

confidence: 99%

A Novel Cortical Mechanism for Top-Down Control of Water Intake

et al. 2020

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“…In the amygdala, NO is additionally linked with modulation of anxiety levels and other emotional processes (Shindou et al, 1993, Yao et al, 2007). It should be noted that the largest neurons in the primate IM were NOS+, and a recent study showed that large IM neurons — which are distinct from small spiny IM neurons — relay noxious sensory information, and can innervate other inhibitory neurons in basal and lateral amygdalar nuclei (Bienvenu et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

The intercalated nuclear complex of the primate amygdala

et al. 2016

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The organization of the inhibitory intercalated cell masses (IM) of the primate amygdala is largely unknown despite their key role in emotional processes. We studied the structural, topographic, neurochemical and intrinsic connectional features of IM neurons in the rhesus monkey brain. We found that the intercalated neurons are not confined to discrete cell clusters, but form a neuronal net that is interposed between the basal nuclei and extends to the dorsally-located anterior, central, and medial nuclei of the amygdala. Unlike the IM in rodents, which are prominent in the anterior half of the amygdala, the primate inhibitory net stretched throughout the antero-posterior axis of the amygdala, and was most prominent in the central and posterior extent of the amygdala. There were two morphologic types of intercalated neurons: spiny and aspiny. Spiny neurons were the most abundant; their somata were small or medium size, round or elongated, and their dendritic trees were round or bipolar, depending on location. The aspiny neurons were on average slightly larger and had varicose dendrites with no spines. There were three non-overlapping neurochemical populations of IM neurons, in descending order of abundance: 1. Spiny neurons that were positive for the striatal associated dopamine- and cAMP-regulated phosphoprotein (DARPP-32+); 2. Aspiny neurons that expressed the calcium-binding protein calbindin (CB); and 3. Aspiny neurons that expressed nitric oxide synthase (NOS+). The unique combinations of structural and neurochemical features of the three classes of IM neurons suggest different physiological properties and function. The three types of IM neurons were intermingled and likely interconnected in distinct ways, and were innervated by intrinsic neurons within the amygdala, or by external sources, in pathways that underlie fear conditioning and anxiety.

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Osmotic regulation of neuronal nitric oxide synthase expression in the rat amygdala: Functional role for nitric oxide in adaptive responses?

Cited by 7 publications

References 36 publications

Water deprivation induces hypoactivity in rats independently of oxytocin receptor signaling at the central amygdala

Water deprivation induces hypoactivity in rats independently of oxytocin receptor signaling at the central amygdala

A Novel Cortical Mechanism for Top-Down Control of Water Intake

The intercalated nuclear complex of the primate amygdala

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