Osmotic tolerance and membrane permeability characteristics of rhesus monkey (Macaca mulatta) spermatozoa

Ağca, Yüksel; Mullen, Steven F.; Liu, Jun; Johnson-Ward, Jill; Gould, Kenneth G.; Chan, Anthony W.S.; Critser, John K.

doi:10.1016/j.cryobiol.2005.04.004

Cited by 45 publications

(41 citation statements)

References 52 publications

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“…where V w (μm 3 ) is the volume of water inside the cell, L p is the water permeability (cm/ sec•atm), A is the surface area of the cell (μm 2 ), R is the universal gas constant ((μm 3 •atm)/ (mol•K)), T is the absolute temperature (K), M e and M i are the external and internal osmolality (osmolal), respectively, N s is the number of moles of permeating solute inside the cell, P s is the solute permeability (cm/s), and and are the external and internal osmolalites of the permeating CPA (osmolal), respectively. Model solutions were fit to experimental data to obtain values of the water (L p ) and solute (P s ) permeability of βTC-tet cells using a least-squares fit (Nelder-Mead Simplex direct routine) with the aid of Matlab (MathWorks, Natick, MA).…”

Section: Membrane Permeability Parametersmentioning

confidence: 99%

“…Transport across the cell membrane of the dual solute system of 3 M DMSO and 3 M PD was modeled as a single component system of the slower permeant, DMSO. An osmotic virial equation model was used to account for the non-ideality of the 6 M DMSO solution osmolality as described by Elliott et al [12] using the quadratic form of the equation, (3) where c i is the molal concentration of solute i (moles of solute per kilogram of solvent), B i is the second osmotic virial coefficient, taken as 0.0843 (moles of solute per kilogram of solvent) −1 for DMSO as per Elliott et al [12].…”

Section: Membrane Permeability Parametersmentioning

confidence: 99%

“…The inactive cell volume, V b , was determined from the Boyle van't Hoff relationship [17], (4) where π e is the extracellular osmolality of the solution (osmolal), V b is the osmotically inactive cell volume (μm 3 ) and V o and are the isotonic cell volume (μm 3 ) and isotonic extracellular osmolality (osmolal), respectively. βTC-tet cells were exposed to various anisosmotic NaCl solutions, allowed to equilibrate, and then measured for their volume using a Coulter Counter®.…”

Section: Membrane Permeability Parametersmentioning

confidence: 99%

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Cryoprotectant delivery and removal from murine insulinomas at vitrification-relevant concentrations

2007

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Development of optimal cryopreservation protocols requires delivery and removal of cryoprotective agents (CPAs) in such a way that negative osmotic and cytotoxic effects on cells are minimized. This is especially true for vitrification, where high CPA concentrations are employed. In this study, we report on the determination of cell membrane permeability parameters for water (L p ) and solute (P s ), and on the design and experimental verification of CPA addition and removal protocols at vitrification-relevant concentrations for a murine insulinoma cell line, βTC-tet cells. Using membrane permeability values and osmotic tolerance limits, mathematical modeling and computer simulations were used to design CPA addition and removal protocols at high concentrations. The cytotoxic effects of CPAs were also evaluated. Cells were able to tolerate the addition and removal of 2.5 M dimethyl sulfoxide (DMSO) and 2.5 M 1,2 propanediol (PD) in single steps, but required multi-step addition and removal with 3.0 M DMSO, 3.0 M PD, and a vitrification-relevant concentration of 3.0 M DMSO+3.0M PD. Cytotoxicity studies revealed that βTC-tet cells were able to tolerate the presence of single component 6.0 M DMSO and 6.0 M PD and to a lesser extent 3.0 M DMSO+3.0 M PD. These results determine the time and concentration domain of CPA exposure that cells can tolerate and are essential for designing cryopreservation protocols for free cells as well as cells in engineered tissues. KeywordsCryopreservation; Vitrification; Membrane permeability; Mouse insulinomas; Cryoprotectant addition-removal; Cryoprotectant cytotoxicityThe need for long-term storage is a critical issue for the preservation of cells, natural and engineered tissues and possibly organs. Cryopreservation may offer a clinically relevant solution by preservation at cryogenic temperatures allowing for off-the-shelf availability, sterility testing, and quality control monitoring. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. permeabilities. When a cell is exposed to a hypertonic solution of a permeating solute, there is an initial abrupt shrinkage caused by water efflux determined by L p , followed by a slower return to normal volume as the solute permeates at a rate determined by P s , which is generally referred to as shrink/swell behavior [17]. To prevent large osmotically induced changes in cell volume and high water fluxes through the cell membrane which can cause damage to cells [20], CPAs are generally loaded and removed in a stepwise manner. Chemical toxicity can also be reduced by lowering the temperature or reducing...

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Section: Membrane Permeability Parametersmentioning

confidence: 99%

Section: Membrane Permeability Parametersmentioning

confidence: 99%

Section: Membrane Permeability Parametersmentioning

confidence: 99%

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Cryoprotectant delivery and removal from murine insulinomas at vitrification-relevant concentrations

2007

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“…A broad tolerance of macaque sperm to conditions required for cryopreservation has also been noted in studies of other sperm parameters such as osmotic stress, water permeability of membranes at room and freezing Effect of LDL and HDL on rhesus monkey sperm QX Dong et al 462 temperatures, and addition of cryoprotectants. [16][17][18] Differences in optimal LDL concentrations for various species could be due to their difference in composition of the sperm plasma membranes. 9 Because LDL consists of 86%-89% lipids and 12.5% proteins, 19 lipids have been the main focus for the study of the protective role of egg yolk.…”

Section: Discussionmentioning

confidence: 99%

“…When samples were frozen without permeable cryoprotectant, LDL in a concentration range of 10%-30% yielded similar post-thaw motility (33%-40%) and AMI (17)(18)(19)(20)(21)(22)(23)(24) as that of samples frozen in 20% egg yolk alone (39%67% for motility and 2469 for AMI). However, LDL at concentrations lower than 8% reduced post-thaw motility and AMI when compared with controls at 20% egg yolk (P,0.05; Figure 2).…”

Section: Evaluation Of Ldl Concentrationmentioning

confidence: 93%

The role of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) in comparison with whole egg yolk for sperm cryopreservation in rhesus monkeys

Dong¹,

Rodenburg²,

Hill³

et al. 2011

Asian J Androl

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Low-density lipoprotein (LDL) extracted from hen egg yolk has recently been considered to be superior to whole egg yolk in sperm cryopreservation of various animal species. Meanwhile, there was a notion that high-density lipoprotein (HDL) in egg yolk may have a negative effect on post-thaw survival. The role of LDL and HDL in sperm cryopreservation of rhesus monkeys has not been explored. The present study evaluates their effect in comparison with egg yolk with or without the addition of permeable cryoprotectant (glycerol) on sperm cryopreservation of rhesus macaques. In addition, various additives intended to change the lipid composition of LDL-sperm membrane complex have also been tested for their effectiveness in preserving post-thaw viability. Our findings indicated that LDL is the main component in egg yolk that is responsible for its protective role for sperm cryopreservation in rhesus monkeys. Regardless of the presence or absence of glycerol, the protective role of LDL is similar to that of egg yolk and we did not observe any superiority in post-thaw survival with LDL when compared to egg yolk. Modifying the lipid composition of LDL-sperm membrane complex with the addition of cholesterol, cholesterol loaded cyclodextrin and phosphatidylcholine also did not yield any improvements in post-thaw survival; while addition of methyl-b-cyclodextrin reduced post-thaw motility. HDL plays a neutral role in sperm cryopreservation of rhesus monkeys. The present study suggests that egg yolk may still hold advantages when compared with LDL as effective components in extenders for sperm cryopreservation in rhesus monkeys.

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The Science of Cryobiology

Mullen¹,

Critser²

2007

Oncofertility Fertility Preservation for Cancer Survivors

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Osmotic tolerance and membrane permeability characteristics of rhesus monkey (Macaca mulatta) spermatozoa

Cited by 45 publications

References 52 publications

Cryoprotectant delivery and removal from murine insulinomas at vitrification-relevant concentrations

Cryoprotectant delivery and removal from murine insulinomas at vitrification-relevant concentrations

The role of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) in comparison with whole egg yolk for sperm cryopreservation in rhesus monkeys

The Science of Cryobiology

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