OsMS1 functions as a transcriptional activator to regulate programmed tapetum development and pollen exine formation in rice

Yang, Zhengfu; Liu, Ling; Sun, Lianping; Yü, Ping; Zhang, Peipei; Abbas, Adil; Xiang, Xiaojiao; Wu, Weixun; Zhang, Yingxin; Zhang, Yingxin; Cheng, Shifeng

doi:10.1007/s11103-018-0811-0

Cited by 73 publications

(65 citation statements)

References 44 publications

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“…Anthers of wild‐type and tip3 mutants at all developmental stages were collected in liquid nitrogen (Zhang et al ., 2017b). Total RNA was isolated and cDNA was synthesized as reported previously (Yang et al ., ). qPCR was performed on a Roche LightCycler 480 device using SYBR Premix Ex Taq II (TaKaRa, Kyoto, JAPAN).…”

Section: Methodsmentioning

confidence: 97%

“…A TUNEL (terminal‐deoxynucleoitidyl transferase‐mediated nick‐end labeling) assay was performed as described previously to investigate the DNA fragmentation in wild‐type and tip3 mutant anthers (Yang et al ., ). All sections were dewaxed in xylene and digested in proteinase K. In situ nick‐end labeling of nuclear DNA fragmentation was performed using the DeadEnd Fluorometric TUNEL system kit (Promega, Wisconsin, USA) according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 97%

“…Pollen grains were observed and photographed with a Leica DM2500 microscope. Spikelets at different developmental stages were selected to perform semi‐thin sections and TEM assay as described previously (Li et al ., ; Yang et al ., , ). Development stages of rice anther were determined as previously described (Zhang and Wilson, ; Zhang et al ., ).…”

Section: Methodsmentioning

confidence: 99%

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TDR INTERACTING PROTEIN 3, encoding a PHD‐finger transcription factor, regulates Ubisch bodies and pollen wall formation in rice

et al. 2019

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Summary Male reproductive development involves a complex series of biological events and precise transcriptional regulation is essential for this biological process in flowering plants. Several transcriptional factors have been reported to regulate tapetum and pollen development, however the transcriptional mechanism underlying Ubisch bodies and pollen wall formation remains less understood. Here, we characterized and isolated a male sterility mutant of TDR INTERACTING PROTEIN 3 (TIP3) in rice. The tip3 mutant displayed smaller and pale yellow anthers without mature pollen grains, abnormal Ubisch body morphology, no pollen wall formation, as well as delayed tapetum degeneration. Map‐based cloning demonstrated that TIP3 encodes a conserved PHD‐finger protein and further study confirmed that TIP3 functioned as a transcription factor with transcriptional activation activity. TIP3 is preferentially expressed in the tapetum and microspores during anther development. Moreover, TIP3 can physically interact with TDR, which is a key component of the transcriptional cascade in regulating tapetum development and pollen wall formation. Furthermore, disruption of TIP3 changed the expression of several genes involved in tapetum development and degradation, biosynthesis and transport of lipid monomers of sporopollenin in tip3 mutant. Taken together, our results revealed an unprecedented role for TIP3 in regulating Ubisch bodies and pollen exine formation, and presents a potential tool to manipulate male fertility for hybrid rice breeding.

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Section: Methodsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

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TDR INTERACTING PROTEIN 3, encoding a PHD‐finger transcription factor, regulates Ubisch bodies and pollen wall formation in rice

et al. 2019

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“…OsMS1 regulates tapetal programmed cell death and pollen exine formation in rice; it functions as a transcriptional activator and interacts with known tapetal regulatory factors through its plant homeodomain [24]. Loss-of-functionof OsTKPR1 delays degeneration of the tapetum, reduces the lipid level of the anther cuticle, disrupts the Ubisch body and pollen exine formation, and results in complete male sterility [25].…”

Section: Discussionmentioning

confidence: 99%

ARABIDOPSIS POLLEN ABORTION MUTANT 2, Encoding an Unknown Protein with Three WD40-Repeats, Is Essential for Late Pollen Mature in Arabidopsis thaliana

Zhang

Chen

et al. 2019

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Background In flowering plants, pollen formation is a very complex process. It is strictly regulated by various genetic factors. Some of these factors have been identified, but the regulatory mechanism concerning the process of late pollen maturing remains unknown. Results Previously we identified and reported an ARABIDOPSIS POLLEN ABORTION MUTANT 2 (APAM2) gene which was required for pollen formation in Arabidopsis thaliana. In this study, we further analysed the phenotype of atapam2 and the subcellular localisation of AtAPAM2. Mutation in AtAPAM2 drastically affected male gametophytic function, impacted the late pollen mature process, and resulted in mature pollen grains from atapam2/+ plants being shrivelled and dead. It was expressed among seedlings, roots, stems, leaves, siliques, flowers, and pollen grains. However, its expression levels in flowers and pollen grains were much higher than in other tissues. Gene AtAPAM2 encodes an unknown protein with three WD40-repeats with localisation in plasma membrane and nucleus, and homologous proteins exist in many species. Conclusions We determined that AtAPAM2 may play a role in pollen formation and other development processes by interacting with other proteins, but we did not identify the protein that interacts with it.

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“…A conserved glycerol-3-phosphate acyltransferase gene OsGPAT3 plays an important role in regulating anther wall degradation and pollen exocrine formation in rice [7]. Gene OsMS1 regulates tapetal programmed cell death and pollen exine formation in rice; it functions as a transcriptional activator and interacts with known tapetal regulatory factors through its plant homeodomain [8]. Loss-offunctionof OsTKPR1 delays degeneration of the tapetum, reduces the lipid level of the anther cuticle, disrupts the Ubisch body and pollen exine formation, and results in complete male sterility [9].…”

Section: Introductionmentioning

confidence: 99%

ARABIDOPSIS POLLEN ABORTION MUTANT 2, Encoding an Unknown Protein with Three WD40-Repeats, Is Essential for Late Pollen Mature in Arabidopsis thaliana

Zhang

Chen

et al. 2019

Preprint

View full text Add to dashboard Cite

Background In flowering plants, pollen formation is a very complex process. It is strictly regulated by various genetic factors. Some of these factors have been identified, but the regulatory mechanism concerning the process of late pollen maturing remains unknown.Results Previously we identified and reported an ARABIDOPSIS POLLEN ABORTION MUTANT 2 (APAM2) gene which was required for pollen formation in Arabidopsis thaliana. In this study, we further analysed the phenotype of atapam2 and the expression pattern of AtAPAM2. Mutation in AtAPAM2 drastically affected male gametophytic function, impacted the late pollen mature process, and resulted in mature pollen grains from atapam2/+ plants being shrivelled and dead. It was expressed among seedlings, roots, stems, leaves, siliques, flowers, and pollen grains. However, its expression levels in flowers and pollen grains were much higher than in other tissues. AtAPAM2 encodes an unknown protein with three WD40-repeats with localisation in plasma membrane and nucleus, and homologous proteins exist in many species.Conclusions We determined that AtAPAM2 may play a role in pollen formation and other development processes by interacting with other proteins, but we did not identify the protein that interacts with it.

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OsMS1 functions as a transcriptional activator to regulate programmed tapetum development and pollen exine formation in rice

Cited by 73 publications

References 44 publications

TDR INTERACTING PROTEIN 3, encoding a PHD‐finger transcription factor, regulates Ubisch bodies and pollen wall formation in rice

TDR INTERACTING PROTEIN 3, encoding a PHD‐finger transcription factor, regulates Ubisch bodies and pollen wall formation in rice

ARABIDOPSIS POLLEN ABORTION MUTANT 2, Encoding an Unknown Protein with Three WD40-Repeats, Is Essential for Late Pollen Mature in Arabidopsis thaliana

ARABIDOPSIS POLLEN ABORTION MUTANT 2, Encoding an Unknown Protein with Three WD40-Repeats, Is Essential for Late Pollen Mature in Arabidopsis thaliana

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