OsPDR20 is an ABCG metal transporter regulating cadmium accumulation in rice

He, Ling; Li, Chao; Sun, Di; Yang, Zhimin

doi:10.1016/j.jes.2022.09.021

Cited by 19 publications

(3 citation statements)

References 54 publications

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“…A possible role of PDR in copper detoxification may involve utilizing a similar efflux mechanism to export copper away from the cytosol and plasma membrane. The suppression of PDR in certain species treated with cadmium or lead resulted in severe growth defects, establishing PDR as a crucial component in heavy metal detoxification [ 51 , 52 ]. Hormones such as Jasmonic acid and ABA induced the upregulation of Ospdr9, suggesting that PDR may also play a secondary role in mediating the general stress response [ 49 , 53 ].…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling of Jack Pine (Pinus banksiana) under copper stress: Identification of genes associated with copper resistance

Moy,

Czajka,

Michael

et al. 2024

PLoS ONE

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Understanding the genetic response of plants to copper stress is a necessary step to improving the utility of plants for environmental remediation and restoration. The objectives of this study were to: 1) characterize the transcriptome of Jack Pine (Pinus banksiana) under copper stress, 2) analyze the gene expression profile shifts of genotypes exposed to copper ion toxicity, and 3) identify genes associated with copper resistance. Pinus banksiana seedlings were treated with 10 mmoles of copper and screened in a growth chamber. There were 6,213 upregulated and 29,038 downregulated genes expressed in the copper resistant genotypes compared to the susceptible genotypes at a high stringency based on the false discovery rate (FDR). Overall, 25,552 transcripts were assigned gene ontology. Among the top upregulated genes, the response to stress, the biosynthetic process, and the response to chemical stimuli terms represented the highest proportion of gene expression for the biological processes. For the molecular function category, the majority of expressed genes were associated with nucleotide binding followed by transporter activity, and kinase activity. The majority of upregulated genes were located in the plasma membrane while half of the total downregulated genes were associated with the extracellular region. Two candidate genes associated with copper resistance were identified including genes encoding for heavy metal-associated isoprenylated plant proteins (AtHIP20 and AtHIP26) and a gene encoding the pleiotropic drug resistance protein 1 (NtPDR1). This study represents the first report of transcriptomic responses of a conifer species to copper ions.

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Section: Discussionmentioning

confidence: 99%

Gene expression profiling of Jack Pine (Pinus banksiana) under copper stress: Identification of genes associated with copper resistance

Moy,

Czajka,

Michael

et al. 2024

PLoS ONE

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“…OsPDR20 is strongly induced in roots and shoots of seedling under Cd stress. Knockdown OsPDR20 increasing the Cd accumulation in plant (Li, et al 2024). According to previous predict the structure of MDRs is reverse with PDRs (Jasinski, et al 2003), and OsMDR4 is localized to plasma membrane in this study (Fig.…”

Section: Discussionmentioning

confidence: 99%

The Multidrug Resistance Protein OsMDR4 is Involved in Cadmium Absorption in Rice (Oryza sativa L.)

Xie,

Hao,

Zhao

et al. 2023

Preprint

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Key message OsMDR4 is an ABC transporter which localized to plasma membrane and mainly expressed in root to mediate the Cd absorption in rice. Abstract Cadmium(Cd) is one of heavy metals which threaten crop security production. The transporters in root are key factors involved in metal absorption. However, there are a large amount of Cd transporter in rice root remains uncharted. Here, we report a member of multidrug resistance protein OsMDR4 mediating the Cd absorption in rice. Heterologous overexpressing OsMDR4 in yeast increased the sensitivity to Cd treatment and Cd content in the cells. Consistantly, the Cd concentration of the mdr4 mutants is lower than that in wild type, in both root and shoot. In addition, the Vmax values of Cd uptake in mdr4 mutants are much lower than that of wild type by uptake kinetics analysis. OsMDR4 is mainly expressed in epidermis and root hairs of rice roots during seedling stage, and flower organs at flowering stage. Further more, the expression level of OsMDR4 in seedling root is induced by Cd supply. As a transporter OsMDR4 is localized to plasma membrane of rice cells. Taken together, we characterized a novel Cd transporter OsMDR4 which had a significant contribution in Cd influx into root.

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“…Wang等人 [55] 在拟南芥中表达杨树(Populus tomentosa) ABCG36基因, 发现在Cd胁迫处理下, 转化株系的生长明显优于野生型, 且体内Cd含量少于野生型, 证明了 PtoABCG36转运蛋白可以作为一种镉的挤压泵将Cd外排, 通过降低植物中Cd的含量来提高杨树对Cd的耐受性. Li等人 [56] 研究了一个功能未知ABCG转运蛋白 OsPDR20对Cd的转运能力, 发现在Cd胁迫条件下水稻幼苗根和芽中的OsPDR20被强烈诱导; RNAi敲低 OsPDR20导致水稻Cd积累, 根茎的伸长量和生物量显著降低; 田间试验表明, RNAi植株的生长发育受到了影响, 小穗较小, 育性较低, 镉的积累水平显著升高.…”

Section: Abcg转运蛋白对非生物胁迫的响应unclassified