OsRRM, an RNA-Binding Protein, Modulates Sugar Transport in Rice (Oryza sativa L.)

Liu, Qiaoquan; Xu, Lina; Wang, Wei; Jia, Shuwen; Jin, Sukui; Gao, Jinliang

doi:10.3389/fpls.2020.605276

Cited by 11 publications

(9 citation statements)

References 77 publications

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“…Furthermore, expression in the nodes and stems has been thought to relate to cell proliferation/expansion and nutrient transport. For example, our previous study reported that a spin-like gene, OsRRM , which modulates sugar transport, had a similar expression profile to OsNAC129 in stems, and the osrrm mutant displayed reduced plant height and grain size, similar to the phenotypes observed in OsNAC129 -OE plants ( Liu et al, 2020 ). Very recently, another group reported that OsNAC129 and its interactor OsNAC127 do indeed participate in grain filling regulation by directly targeting sugar transporter genes including OsMST6 and OsSWEET4 ( Ren et al, 2021 ).…”

Section: Discussionmentioning

confidence: 77%

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OsNAC129 Regulates Seed Development and Plant Growth and Participates in the Brassinosteroid Signaling Pathway

et al. 2022

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Grain size and the endosperm starch content determine grain yield and quality in rice. Although these yield components have been intensively studied, their regulatory mechanisms are still largely unknown. In this study, we show that loss-of-function of OsNAC129, a member of the NAC transcription factor gene family that has its highest expression in the immature seed, greatly increased grain length, grain weight, apparent amylose content (AAC), and plant height. Overexpression of OsNAC129 had the opposite effect, significantly decreasing grain width, grain weight, AAC, and plant height. Cytological observation of the outer epidermal cells of the lemma using a scanning electron microscope (SEM) revealed that increased grain length in the osnac129 mutant was due to increased cell length compared with wild-type (WT) plants. The expression of OsPGL1 and OsPGL2, two positive grain-size regulators that control cell elongation, was consistently upregulated in osnac129 mutant plants but downregulated in OsNAC129 overexpression plants. Furthermore, we also found that several starch synthase-encoding genes, including OsGBSSI, were upregulated in the osnac129 mutant and downregulated in the overexpression plants compared with WT plants, implying a negative regulatory role for OsNAC129 both in grain size and starch biosynthesis. Additionally, we found that the expression of OsNAC129 was induced exclusively by abscisic acid (ABA) in seedlings, but OsNAC129-overexpressing plants displayed reduced sensitivity to exogenous brassinolide (BR). Therefore, the results of our study demonstrate that OsNAC129 negatively regulates seed development and plant growth, and further suggest that OsNAC129 participates in the BR signaling pathway.

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Section: Discussionmentioning

confidence: 77%

“…Southern blot analysis of the osnac129 mutant was performed as described previously ( Liu et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

OsNAC129 Regulates Seed Development and Plant Growth and Participates in the Brassinosteroid Signaling Pathway

et al. 2022

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“…Several sugar transporters have been shown to be regulated posttranscriptionally by the RNA binding protein OsRRM. OsRRM is produced in anthers and has been shown to positively affect steady‐state transcript levels of a suite of transporter genes including MST6 and 8 , TMT1 and 2 , as well as four sucrose transporters SUT1 , 2 , 3 and 4 , probably by stabilising the respective mRNAs (Liu et al ., 2020). SWEET mRNA and protein levels have not been tested in these mutants, yet, based on the findings summarised above, it is tempting to speculate that SWEET11a and 11 b may be coregulated by the same mechanisms and may also be involved in temperature tolerance of male reproductive organs in rice.…”

Section: Discussionmentioning

confidence: 99%

“…3 and 4, probably by stabilising the respective mRNAs (Liu et al, 2020). SWEET mRNA and protein levels have not been tested in these mutants, yet, based on the findings summarised above, it is tempting to speculate that SWEET11a and 11b may be coregulated by the same mechanisms and may also be involved in temperature tolerance of male reproductive organs in rice.…”

Section: Interplay Of Sweet11a and 11b With Other Sugar Transporters ...mentioning

confidence: 99%

OsSWEET11b, a potential sixth leaf blight susceptibility gene involved in sugar transport‐dependent male fertility

Eom

Isoda

et al. 2022

New Phytologist

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Summary SWEETs play important roles in intercellular sugar transport. Induction of SWEET sugar transporters by Transcription Activator‐Like effectors (TALe) of Xanthomonas ssp. is key for virulence in rice, cassava and cotton. We identified OsSWEET11b with roles in male fertility and potential bacterial blight (BB) susceptibility in rice. While single ossweet11a or 11b mutants were fertile, double mutants were sterile. As clade III SWEETs can transport gibberellin (GA), a key hormone for spikelet fertility, sterility and BB susceptibility might be explained by GA transport deficiencies. However, in contrast with the Arabidopsis homologues, OsSWEET11b did not mediate detectable GA transport. Fertility and susceptibility therefore are likely to depend on sucrose transport activity. Ectopic induction of OsSWEET11b by designer TALe enabled TALe‐free Xanthomonas oryzae pv. oryzae (Xoo) to cause disease, identifying OsSWEET11b as a potential BB susceptibility gene and demonstrating that the induction of host sucrose uniporter activity is key to virulence of Xoo. Notably, only three of six clade III SWEETs are targeted by known Xoo strains from Asia and Africa. The identification of OsSWEET11b is relevant for fertility and for protecting rice against emerging Xoo strains that target OsSWEET11b.

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Section: Interplay Of Sweet11a and B With Other Sugar Transporters And Cell Wall Invertasesmentioning

confidence: 99%

OsSWEET11b, a sixth leaf blight susceptibility gene involved in sugar transport-dependent male fertility

Eom

Isoda

et al. 2021

Preprint

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SummarySWEETs play important roles in intercellular sugar transport. Induction of SWEET sugar transporters by transcription activator-like effectors (TALe) of Xanthomonas ssp. is a key factor for bacterial leaf blight (BLB) infection of rice, cassava and cotton. Here, we identified the so far unknown OsSWEET11b with roles in male fertility and BLB susceptibility in rice. While single ossweet11a or b mutants were fertile, double mutants were sterile. Since clade III SWEETs can transport gibberellin (GA), a key hormone for rice spikelet fertility, sterility and BLB susceptibility might be explained by GA transport deficiencies. However, in contrast to the Arabidopsis homologs, OsSWEET11b did not mediate detectable GA transport. Fertility and susceptibility must therefore depend on SWEET11b-mediated sucrose transport. Ectopic induction of OsSWEET11b by designer TALe enables TALe-free Xanthomonas oryzae pv. oryzae (Xoo) to cause disease, identifying OsSWEET11b as a BLB susceptibility gene and demonstrating that the induction of host sucrose uniporter activity is key to virulence of Xoo. Notably, only three of now six clade III SWEETs are targeted by known Xoo strains from Asia and Africa. The identification of OsSWEET11b has relevance in the context of fertility and for protecting rice against emerging Xoo strains that evolve TALes to exploit OsSWEET11b.

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OsRRM, an RNA-Binding Protein, Modulates Sugar Transport in Rice (Oryza sativa L.)

Cited by 11 publications

References 77 publications

OsNAC129 Regulates Seed Development and Plant Growth and Participates in the Brassinosteroid Signaling Pathway

OsNAC129 Regulates Seed Development and Plant Growth and Participates in the Brassinosteroid Signaling Pathway

OsSWEET11b, a potential sixth leaf blight susceptibility gene involved in sugar transport‐dependent male fertility

OsSWEET11b, a sixth leaf blight susceptibility gene involved in sugar transport-dependent male fertility

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