Osseointegration Effect of Micro-Nano Implants Loaded With Kaempferol in Osteoporotic Rats

Abstract

Objective: To investigate the effect of osseointegration of kaempferol loaded on the surface of micro-nanomorphic implants in ovariectomized rats.Methods: Titanium flakes were polished to obtain the PT group, anodized and acid-etched to obtain the NT and WNT groups, loaded with kaempferol to obtain the KNT and KWNT groups, and spin-coated on chitosan-gelatin composite film to obtain the KNT-CG and KWNT-CG groups. In vitro experiments were performed to observe the physicochemical properties of the titanium tablets in each group through scanning electron microscopy and contact angle experiments. The cytotoxicity and drug release pattern were observed using CCK-8 and drug release assays. An osteoporosis rat model was established. Pure titanium implants were divided into PT, NT, WNT, KNT-CG, and KWNT-CG groups after the same treatment and used in the in vivo experiments and then implanted in the femur of mice in each group. After 4 weeks, all samples were collected for toluidine blue staining, micro-computed tomography scanning, and bone morphometry analysis to evaluate their osteogenic properties.Results: According to scanning electron microscopy, the surface of the titanium flakes had a micro-nano morphology in the WNT group and the KNT and KWNT groups were functionally loaded with kaempferol. In CCK-8 and drug release experiments, the loaded kaempferol and gelatin composite membranes showed no significant toxic effects on cells. The drug release time in the KNT-CG and KWNT-CG groups was significantly longer than that in the KNT and KWNT groups, with the release time in the KWNT-CG group reaching 15 days. In vivo experiments micro-computed tomography and bone morphometry analysis showed that the osteoporosis model had been successfully constructed. The bone volume fraction around the implant increased. Toluidine blue staining showed new bone formation and a significantly increased number of bone trabeculae.Conclusion: Kaempferol micro-nanocomposite coating improved the osseointegration ability of implants in osteoporotic rats.

Molecular mechanism of Achyranthis bidentatae radix and Morindae officinalis radix in osteoporosis therapy: An investigation based on network pharmacology, molecular docking, and molecular dynamics simulations

“…They can play an anti-OP role through multiple mechanisms such as promoting osteoblast differentiation, inhibiting osteoclast formation, and resisting iron overload. Presently, they are being explored as monomers or in combination with other compounds for potential application in OP therapy [ 20 , 21 , [35] , [36] , [37] , [38] , [39] , [40] , [41] , [42] , [43] ].…”

Transcriptomics-based Exploration of the Mechanism of Kaempferol in the Treatment of Osteoporosis in Ovariectomized Rats

“…2 Modern pharmacological studies have shown that kaempferol is an extract of many herbal medicines like Eucommia, Epimedium, Rhizoma Drynariae, and Achyranthes Bidentata. 3 Some experiments proved that kaempferol can regulate bone metabolism balance and improve bone density by increasing osteoblast activity while decreasing osteoclast activity. [4][5][6][7][8] Our preliminary experiments found that RPN1 may serve as a new target for kaempferol to improve osteoporosis.…”

Strontium-doping promotes bone bonding of titanium implants in osteoporotic microenvironment

“…Alkali heat treatment is a simple and stable method to construct Sr-containing coating on the surface of titanium implants, which leads to formation of nano-scale surface structures ( Wang et al, 2018 ; Wang et al, 2020 ; Okuzu et al, 2021 ). Nanoscale surface features increase protein adsorption, stimulate osteoblast migration, and accelerate integration of bone and implants ( Ding et al, 2020 ; Shu et al, 2020 ; Wang et al, 2022 ). By adjusting reaction conditions, the synergy of the two strategies provide a new idea for promoting osteoporotic bone bonding.…”