Ossification of the Ligaments in the Cervical Spine, Including Ossification of the Anterior Longitudinal Ligament, Ossification of the Posterior Longitudinal Ligament, and Ossification of the Ligamentum Flavum

Ohara, Yukoh

doi:10.1016/j.nec.2017.09.018

Cited by 29 publications

(24 citation statements)

References 61 publications

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“…Since OLF only occured in limited areas of the world, genetic factors were considered as being more important in pathogenetic process. Several studies also strongly supported the view that OLF genetic component play an important role in OLF 1,2,7 . However, the speci c pathophysiology mechanism of this disease is still unknown.…”

Section: Introductionmentioning

confidence: 74%

IGF-2 Modified by the m6A Demethylation Enzyme ALKBH5 in the Ossification of the Ligamentum Flavum

Kuang

Wang

Qiu

et al. 2020

Preprint

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Background: Ossification of the ligamentum flavum (OLF) is a pathological heterotopic ossification of the paravertebral ligament. However, the specific pathophysiology mechanism of this disease is still unknown. The m6A methylation and its potential functions in OLF remain to be unexplored. Method: In this study, we performed a transcriptome-wide methylation analysis using the OLF and normal ligaments to explore the mechanism of OLF. Common and region-specific methylation have different preferences for methylation site selection and thereby different impacts on their biological functions. We screened out the methylase-ALKBH5, and by promoting or inhibiting its expression, observing the content of m6A, and measuring the ossification of ligaments, and then measuring IGF expression situation by alizarin red staining, alkaline phosphatase, immunofluorescence, immunohistochemistry, etc. Result: MeRIP-seq and qPCR showed that the m6A methylation level of OLF group was usually higher than that of control group. In addition, we found that ALKBH5 is an important demethyltransferase in OLF, which promotes the expression of m6A. ALKBH5 promotes the expression of IGF-2, which in turn promotes osteogenesis in OLF.Conclusion: Overall, we provided a region-specific map of m6A methylation and characterized the distinct features of specific and common methylation in OLF, and we proved that IGF-2 can be regulated by ALKBH5 to promote the process of OLF.

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Section: Introductionmentioning

confidence: 74%

IGF-2 Modified by the m6A Demethylation Enzyme ALKBH5 in the Ossification of the Ligamentum Flavum

Kuang

Wang

Qiu

et al. 2020

Preprint

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“…Miyazawa and Akiyama (4) reviewed 50 cases of OLF in the cervical spine that were reported between 1962 and 2005. The mean age of the patients was 56.3 years, with a male/female ratio of 1.44:1 (16). Sensorimotor deficits were common in those patients with OLF.…”

Section: Discussionmentioning

confidence: 99%

Ossification of the ligamentum flavum in the upper cervical spine: A report of two cases and literature review

Rui

Huang

2020

Exp Ther Med

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Ossification of the ligamentum flavum (OLF) is rarely observed in the cervical spine, particularly not in the upper cervical spine. The present report describes two cases of OLF combined with cervical myelopathy and provides a literature review with a comparative analysis of previous studies and the present cases. Pre-operative CT, MRI and histological examination were performed to confirm the diagnosis of these patients (A 37-year-old female and 63-year-old male). In both cases, histological examination of surgical specimens revealed OLF. The symptoms of the two patients significantly improved after surgery and no recurrence was observed at 13 months after surgery. Furthermore, the clinical outcomes were satisfactory and the internal fixation was solid in the two cases. Review of the literature suggested that further research is required to reveal the optimal treatment methods for upper cervical OLF.

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“…Although radiological evaluation may be useful in assessing the risk of difficult intubation, it is still not recommended because OALL of the cervical spine is a relatively common condition that is only occasionally associated with difficult intubation [14]. Currently, the etiology and pathogenesis of OALL remain unclear, but this condition is strongly associated with frequently diagnosed metabolic abnormalities and joint degeneration [1]. In addition, it may be related to increased cervical motion or trauma.…”

Section: Discussionmentioning

confidence: 99%

“…Diffuse idiopathic skeletal hyperplasia (DISH), also named as "Forestier's disease," is a rare idiopathic spinal disease characterized by a "flowing" ossification of the anterior longitudinal ligament (OALL) of the spine with an unknown etiology [1]. OALL of the cervical spine is common in patients over the age of 50 years, with a prevalence of approximately 15-20% in the elderly [2,3].…”

Section: Introductionmentioning

confidence: 99%

Ossification of the cervical anterior longitudinal ligament is an underdiagnosed cause of difficult airway: a case report and review of the literature

Liu

Yang

et al. 2020

BMC Anesthesiol

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Background: Ossification of the anterior longitudinal ligament (OALL) of the cervical spine is a common, but rarely symptomatic, condition mostly observed in the geriatric population. Although the condition usually requires no intervention, it could lead to a difficult airway and compromise the patient's safety. Case presentation: Here, we describe the case of a 50-year-old man with cervical myelopathy and OALL that resulted in difficult endotracheal intubation after induction of anesthesia. Radiography and magnetic resonance imaging findings showed OALL, with prominent osteophytes involving four cervical vertebrae, a bulge in the posterior pharyngeal wall, and a narrow pharyngeal space. Airtraq® laryngoscope-assisted intubation was accomplished with rapid induction under sevoflurane-inhaled anesthesia. Conclusion: Anesthesiologists should understand that OALL of the cervical spine could cause a difficult airway. However, it is difficult to recognize asymptomatic OALL on the basis of routine airway evaluation guidelines. For susceptible populations, a thorough evaluation of the airway, based on imaging studies and a history of compression symptoms, should be considered whenever possible. In case of unanticipated difficult intubation, anesthesiologists should refer to guidelines for unanticipated difficult airway management and identify OALL of the cervical spine as the cause.

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Ossification of the Ligaments in the Cervical Spine, Including Ossification of the Anterior Longitudinal Ligament, Ossification of the Posterior Longitudinal Ligament, and Ossification of the Ligamentum Flavum

Cited by 29 publications

References 61 publications

IGF-2 Modified by the m6A Demethylation Enzyme ALKBH5 in the Ossification of the Ligamentum Flavum

IGF-2 Modified by the m6A Demethylation Enzyme ALKBH5 in the Ossification of the Ligamentum Flavum

Ossification of the ligamentum flavum in the upper cervical spine: A report of two cases and literature review

Ossification of the cervical anterior longitudinal ligament is an underdiagnosed cause of difficult airway: a case report and review of the literature

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Ossification of the Ligaments in the Cervical Spine, Including Ossification of the Anterior Longitudinal Ligament, Ossification of the Posterior Longitudinal Ligament, and Ossification of the Ligamentum Flavum

Cited by 29 publications

References 61 publications

IGF-2 Modified by the m6A&nbsp;Demethylation Enzyme ALKBH5&nbsp;in the Ossification of the Ligamentum Flavum

IGF-2 Modified by the m6A&nbsp;Demethylation Enzyme ALKBH5&nbsp;in the Ossification of the Ligamentum Flavum

Ossification of the ligamentum flavum in the upper cervical spine: A report of two cases and literature review

Ossification of the cervical anterior longitudinal ligament is an underdiagnosed cause of difficult airway: a case report and review of the literature

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IGF-2 Modified by the m6A Demethylation Enzyme ALKBH5 in the Ossification of the Ligamentum Flavum

IGF-2 Modified by the m6A Demethylation Enzyme ALKBH5 in the Ossification of the Ligamentum Flavum