Osteoarthritis model induced by intra-articular monosodium iodoacetate in rats knee

Abstract

PURPOSE:To evaluate the usefulness of a knee osteoarthritis model through functional, radiological and microscopic changes of the synovial membrane. METHODS:Forty eight rats were divided randomly into two groups. The first received 0.9% saline in the joint and corresponded to the control group. The second was submitted to experimental osteoarthritis of the right knee induced by monosodium iodoacetate and corresponded to the osteoarthritis group. All animals were subjected to comparative tests of forced ambulation and joint movements, inability to articulate and tactile allodynia on day 1 post-experiment by forced ambulation (Roto-rod test), joint assessment of disability (weight bearing test) and assessment of tactile allodynia (Von Frey test). After inflammatory induction they were divided into four subgroups corresponding to the scheduled death in 7, 14, 21 and 28 days when they were submitted to radiographic examination of the knee, arthrotomy and collection of the synovial membrane. RESULTS:The osteoarthritis group showed significant differences compared to control group on days 7 and 14 in Roto-rod, in weight bearing and Von Frey tests in all days, and in radiological evaluation. Microscopic examination of the synovial membrane showed abnormalities of inflammatory character at all stages. CONCLUSION:The osteoarthritis induced by intra-articular monosodium iodoacetate in rats knee is a good model to be used in related research, because it provides mensurable changes on joint movements, tactile allodynia, progressive radiological degeneration and microscopic inflammation of the synovial membrane, that represent markers for osteoarthritis evaluation

Psoralen Protects Chondrocytes, Exhibits Anti-Inflammatory Effects on Synoviocytes, and Attenuates Monosodium Iodoacetate-Induced Osteoarthritis

“…Animal experiments were carried out according to the protocols approved by Chongqing University Animal Care and Use Committee. According to the references 19, 20, OA was induced by a single intra-articular knee injection of monosodium iodoacetate (MIA) (Sigma) (6 mg/kg body weight, 50 μL) into the bilateral knee-joints of the Sprague-Dawley (SD) rats (200-250g). Two weeks followed by MIA injection, the rats had their knee-joints treated with 1 mg/kg (body weight) of PSO (MIA+PSO group) and sterile saline solution (MIA group) via intra-articular knee injection daily for 1 week, and once every three days for another 1 week.…”

Contribuição Da Avaliação Da Qualidade Do Sono Em Professores Com História De Tontura: Uma Opção De Avaliação

“…A doença articular degenerativa é denominada de osteoartrose, osteoartrite ou artrite degenerativa, é definida pelo Colégio Americano de Reumatologia como um conjunto de alterações que induzem sintomas e sinais dolorosos articulares associados a defeitos na integridade da cartilagem articular e da membrana sinovial, levando a alterações na margem óssea articular. (MORAIS et al, 2016). É um grupo de doenças e alterações mecânicas que interferem na degradação da cartilagem articular e do osso subcondral, ocasionando diminuição da amplitude dos movimentos.…”

Chondroprotective effects of platelet lysate towards monoiodoacetate-induced arthritis by suppression of TNF-α-induced activation of NF-ĸB pathway in chondrocytes

“…The MIA model is commonly used as an arthritis model mimicking human OA for pain assessment, owing to the consistent pain-like responses throughout the modeling period [23]. It provides measurable changes on joint movements, tactile allodynia, inflammation, and progressive cartilage degeneration that represent markers for OA evaluation [24]. Previous studies reported that the MIA model is useful for evaluating the pathology of non-traumatic OA, pain mechanisms, and therapeutic effects on cartilage [25, 26].…”