Osteoarthritis year in review 2018: clinical

Mandl, Lisa A.

doi:10.1016/j.joca.2018.11.001

Cited by 272 publications

(187 citation statements)

References 39 publications

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“…Primers (shown in Table 2) were synthesized by Ige Biotech Company (Guangzhou, China) and were diluted to 2.5 μM with RNase-free water. The reaction mix (10 μL of SYBR Green Supermix, 0.8 μL of PCR Forward Primer, 0.8 μL of PCR Reverse Primer and 3.4 μL of dH 2 O) was prepared on ice and then was added to a PCR tube containing 5 μL of cDNA template and was centrifuged at 100 g. The reaction procedure was as follows: 1…”

Section: Real-time Pcr Analysismentioning

confidence: 99%

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Protective effects of ten oligostilbenes from Paeonia suffruticosa seeds on interleukin-1β-induced rabbit osteoarthritis chondrocytes

Cen

Liu

et al. 2019

BMC Chemistry

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Background: Paeonia suffruticosa is an important traditional Chinese herb used to treat osteoarthritis (OA) and oligostilbenes are the main active ingredient of the seeds of P. suffruticosa. The monomer trans-resveratrol of this species was demonstrated to have chondroprotective effects as a lead compound for the treatment of osteoarthritis, but it has not been applied due to its low efficacy. Methods: Oligostilbenes were isolated by chromatography and were identified by NMR and HPLC. A rabbit osteoarthritis chondrocyte model was induced by interleukin-1β and was treated with individual drugs to systematically evaluate their effects. Cell Counting Kit 8 was used to test their effects on cell viability, calculate EC 50 and plot a doseresponse curve.Their effects on apoptosis were analyzed by Annexin V and PI staining, and the expression of chondrocyte-specific genes COL2A1, MMP13 and SOX9 was evaluated by real-time PCR. Results: Paeonia suffruticosa seed extract could promote the cell viability of rabbit OA chondrocytes at low concentration and then ten oligostilbenes were isolated from it. Trans-oligostilbenes were better than their cis-forms, trimers and dimers were better than monomers for promoting the cell viability of rabbit osteoarthritis chondrocytes. None of the oligostilbenes was more effective than seed extract at the appropriate concentration; 1 μM oligostilbenes all showed various anti-apoptotic effects. Trans-gnetin H showed the best effect on proliferation and inhibition of MMP13 expression on OA chondrocytes, while trans-viniferin was most effective in promoting the expression of COL2A1 and SOX9. Conclusions: Ten oligostilbenes from P. suffruticosa seed all have certain protective effects on OA chondrocytes at low concentration. The trans-viniferin and some trimers have the potential to be further developed for the treatment of osteoarthritis because they were more effective than resveratrol and diacerein. The synergistic effect that may exist between oligostilbenes also warrants further research.

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Section: Real-time Pcr Analysismentioning

confidence: 99%

“…Osteoarthritis (OA), the most common chronic joint disease, is also one of the most common causes of disability. According to epidemiological studies, almost 15% of the population is affected by OA, and the incidence of OA is increasing by 100,000 people per year worldwide [1,2]. However, there is currently no satisfactory treatment.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of ten oligostilbenes from Paeonia suffruticosa seeds on interleukin-1β-induced rabbit osteoarthritis chondrocytes

Cen

Liu

et al. 2019

BMC Chemistry

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“…As a common degenerative and painful disease of the load-bearing joints, affecting mainly the knees, osteoarthritis (OA) is characterized by localized loss of articular cartilage, synovial inflammation and compensatory remodeling of adjacent bone [1][2][3]. In terms of contribution to years of life lived with disease, OA ranks as the 11th greatest contributor and above ischemic heart disease, asthma, Alzheimer's disease, etc [3,4].…”

Section: Introductionmentioning

confidence: 99%

Protective effect of ethyl acetate fraction from Semen sojae germinatum, the processed sprout of Chinese black soybean, on rat experimental osteoarthritis

Wang

Guo

et al. 2020

BMC Complement Med Ther

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Background: Our previous in vitro study reported that the ethyl acetate fraction (EAF) of Semen sojae germinatum (SSG), the processed sprout of Chinese black soybean, possessed the potent anti-inflammatory and chondroprotective properties. The aim of the present work was to verify the in vivo antiosteoarthritic effect of EAF from SSG on a rat osteoarthritis (OA) model. Methods: A classical rat OA model was surgically induced by anterior cruciate ligament transaction (ACLT). The OA rats were intra-articularly administered EAF from SSG for 8 weeks. The cartilage and synovial tissues were stained with hematoxylin and eosin (HE) to observe the histopathological changes. Safranin O/fast green staining was used to assess the glycosaminoglycan content in cartilage tissue sections. The expression of type II collagen and matrix metalloproteinase (MMP)-13 in cartilage was measured by immunohistochemistry. The apoptotic chondrocytes in the cartilage sections were detected using TUNEL assay. The concentrations of interleukin (IL)-1β and tumor necrosis factor (TNF)-ɑ in synovial fluid were determined using ELISA. Results: Intra-articular administration of EAF from SSG well retained the structure and superficial layer of cartilage tissues, ameliorated cartilage lesion and the degradation of cartilage matrix, including proteoglycan and type II collagen, induced by ACLT operation. The ACLT-induced upregulation of MMP-13 expression in the cartilage tissues was resisted by EAF from SSG. Moreover, EAF from SSG inhibited the ACLT-induced chondrocyte apoptosis. Compared to OA model group, the inflammatory status of synovial membrane was improved, the levels of inflammatory cytokines IL-1β and TNF-ɑ in synovial fluid were decreased in rats administrated with EAF from SSG. Conclusion: These data suggested that EAF from SSG displayed in vivo protective effect on OA development via preventing the degeneration of articular cartilage, inhibiting chondrocyte apoptosis and suppressing synovial inflammation.

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“…Osteoarthritis (OA) as one of the most common chronic joint diseases is characterized by articular cartilage defects accompanied with a chronic inflammatory response in the synovial fluid . Epidemiological statistics evaluates an average annual increase of 100,000 newly diagnosed OA patients worldwide . Accumulating evidence corroborates that dysregulation of chondrocyte apoptosis is a central feature in the pathogenesis of OA, which is attributed to chondrocytes that comprise the only resident cells in the articular cartilage modulating the anabolism and catabolism of both extracellular matrix and articular cartilage .…”

Section: Introductionmentioning

confidence: 99%

Oleanolic acid mitigates interleukin‐1β‐induced chondrocyte dysfunction by regulating miR‐148‐3p‐modulated FGF2 expression

Nie

Ping

2020

The Journal of Gene Medicine

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Background: microRNA (miR)-mediated post-transcriptional repression has been reported in the process of chondrocyte dysfunction. The present study aimed to investigate the molecular mechanisms underlying in oleanolic acid (OLA)-prevented interleukin (IL)-1β-induced chondrocyte dysfunction via the miR-148-3p/fibroblast growth factor-2 (FGF-2) signaling pathway.Methods: Candidate miRs were filtrated using miR microarray assays in chondrocytes with or without IL-1β stimulation. Gene expression of candidate miRs and protein expression of FGF2 were analyzed using a quantitative reverse transcriptasepolymerase chain reaction and western blotting, respectively. Cell growth was evaluated using cell counting kit-8 assays. Cell apoptosis was detected using Annexin V-fluorescein isothiocyanate double staining.Results: Treatment with OLA counteracted IL-1β-evoked chondrocyte growth inhibition, apoptosis, caspase3 production, and release of malondialdehyde (MDA) and 8-hydroxy-2'-deoxyguanosine. Additionally, FGF2 protein expression levels elevated by IL-1β were down-regulated by OLA and transfection with miR-148-3p mimics. IL-1β-induced down-regulation of miR-148-3p in chondrocytes was evaluated by OLA administration. Bioinformatics algorithms and experimental measurements indicated that FGF2 might be a direct target of miR-148-3p. miR-148-3p mimics exhibited equal authenticity of OLA to protect against IL-1β-induced chondrocyte dysfunction. Conclusions:Our present findings highlight a protective effect of OLA on IL-1βinduced chondrocyte dysfunction, and a novel signal cascade comprising the miR-148-3p/FGF2 signaling pathway might be a potential therapeutic target of OLA with respect to preventing the progression of osteoarthritis.

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Osteoarthritis year in review 2018: clinical

Cited by 272 publications

References 39 publications

Protective effects of ten oligostilbenes from Paeonia suffruticosa seeds on interleukin-1β-induced rabbit osteoarthritis chondrocytes

Protective effects of ten oligostilbenes from Paeonia suffruticosa seeds on interleukin-1β-induced rabbit osteoarthritis chondrocytes

Protective effect of ethyl acetate fraction from Semen sojae germinatum, the processed sprout of Chinese black soybean, on rat experimental osteoarthritis

Oleanolic acid mitigates interleukin‐1β‐induced chondrocyte dysfunction by regulating miR‐148‐3p‐modulated FGF2 expression

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