Osteoblast adhesion on nanophase ceramics

Webster, Thomas J.; Siegel, Richard W.; Bizios, Rena

doi:10.1016/s0142-9612(99)00020-4

Cited by 901 publications

(648 citation statements)

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“…materials with nanometrescaled grains) can be used to produce nanometre features on biomaterial surfaces to guide cell behaviour along a desired biological response [9,10]. In bone-regeneration applications, promising results have been obtained with the nanophase materials ceramics and metals, with which increased osteoblast adhesion, proliferation and calcium deposition have been observed compared with conventional materials (i.e.…”

Section: Nanocoatings and Nanophase Materialsmentioning

confidence: 99%

Nanotechnology in regenerative medicine: the materials side

Engel

Michiardi

Navarro

et al. 2008

Trends in Biotechnology

299

205

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Section: Nanocoatings and Nanophase Materialsmentioning

confidence: 99%

Nanotechnology in regenerative medicine: the materials side

Engel

Michiardi

Navarro

et al. 2008

Trends in Biotechnology

299

205

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“…It is important to point out that Webster et al (Ref 7,14,15) observed higher osteoblast proliferation levels on nanostructured ceramics (including TiO 2 ) when compared to those of conventional ceramics. This higher osteoblast proliferation on nanostructured ceramics was attributed to the higher probability of protein adsorption caused by nano and submicron asperities on the nanomaterialsÕ surfaces.…”

Section: Enhanced Biocompatibility Of Nanomaterialsmentioning

confidence: 95%

HVOF-Sprayed Nano TiO2-HA Coatings Exhibiting Enhanced Biocompatibility

et al. 2009

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HVOF-sprayed nanoBiomedical thermal spray coatings produced via high-velocity oxy-fuel (HVOF) from nanostructured titania (n-TiO 2 ) and 10 wt.% hydroxyapatite (HA) (n-TiO 2 -10wt.%HA) powders have been engineered as possible future alternatives to HA coatings deposited via air plasma spray (APS). This approach was chosen due to (i) the stability of TiO 2 in the human body (i.e., no dissolution) and (ii) bond strength values on Ti-6Al-4V substrates more than two times higher than those of APS HA coatings. To explore the bioperformance of these novel materials and coatings, human mesenchymal stem cells (hMSCs) were cultured from 1 to 21 days on the surface of HVOF-sprayed n-TiO 2 and n-TiO 2 -10 wt.%HA coatings. APS HA coatings and uncoated Ti-6Al-4V substrates were employed as controls. The profiles of the hMSCs were evaluated for (i) cellular proliferation, (ii) biochemical analysis of alkaline phosphatase (ALP) activity, (iii) cytoskeleton organization (fluorescent/confocal microscopy), and (iv) cell/substrate interaction via scanning electron microscopy (SEM). The biochemical analysis indicated that the hMSCs cultured on n-TiO 2 -10 wt.%HA coatings exhibited superior levels of bioactivity than hMSCs cultured on APS HA and pure n-TiO 2 coatings. The cytoskeleton organization demonstrated a higher degree of cellular proliferation on the HVOF-sprayed n-TiO 2 -10wt.%HA coatings when compared to the control coatings. These results are considered promising for engineering improved performance in the next generation of thermally sprayed biomedical coatings.

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“…The first, statistically-significant evidence of enhanced adhesion of rat calvaria osteoblasts on flat, nanophase (versus microphase/conventional) ceramics (alumina, titania and hydroxylapatite) as a function of decreasing ceramic grain size was provided only a few years ago [1][2]. Most importantly, the enhanced adhesion was specific to osteoblasts, but neither to fibroblasts nor to endothelial cells [3].…”

Section: Cell Adhesionmentioning

confidence: 99%

Mammalian Cell Interactions with Nanophase Materials

Bizios

2004

MRS Proc.

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Adhesion of differentiated mammalian cells from various hard and soft tissues, including adult mesenchymal stem cells is different on nanophase than on microphase/conventional ceramics (such as alumina, titania and hydroxylapatite) as well as on composites of these ceramics with either poly(L-lactic) acid or poly(methyl) methacrylate. Most importantly, nanophase materials promote selective interactions, for example, of osteoblasts but not of fibroblasts. The type, amount and conformation of adsorbed proteins (such as fibronectin, collagen and vitronectin) are key aspects of the underlying mechanism(s) of subsequent cell interactions with nanophase materials. These cellular/molecular results provide evidence that nanophase biomaterials have the potential for improving the efficacy of implants and for promoting neotissue formation pertinent to tissue engineering, regenerative medicine and other clinical applications.

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Osteoblast adhesion on nanophase ceramics

Cited by 901 publications

References 0 publications

Nanotechnology in regenerative medicine: the materials side

Nanotechnology in regenerative medicine: the materials side

HVOF-Sprayed Nano TiO2-HA Coatings Exhibiting Enhanced Biocompatibility

Mammalian Cell Interactions with Nanophase Materials

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