Osteoblast attachment monitored with a quartz crystal microbalance

Redepenning, Jody; Schlesinger, T. K.; Mechalke, Eric; Puleo, David A.; Bizios, Rena

doi:10.1021/ac00071a008

Cited by 130 publications

(100 citation statements)

References 10 publications

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“…This behavior indicates that the process is not controlled by diffusion but the adsorption rate of osteoblasts on the Ti surface. Redepenning et al (1993) observed similar behavior for a single layer of osteoblasts using the Kanazawa equation (Kanazawa and Gordon, 1985). If these results are compared with the adhesion kinetics for other cellular lines one concludes that, in this case, the kinetics is very slow, since during the testing time there was no observed increase in the frequency due to stiffening of the attached cells (Galli Marxer et al, 2003).…”

Section: Resultssupporting

confidence: 54%

Cell adhesion on Ti surface with controlled roughness

et al. 2015

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ABSTRACT:In this report, the in situ interaction between Saos-2 osteoblast cells and a smooth Ti surface was examined over time. The adhesion kinetics and mechanisms of cellular proliferation were monitored by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). The rate of Saos-2 attachment on Ti surfaces, obtained from the measurements performed with the QCM, is a first-order reaction, with k=2.10. The impedance measurements indicate that in the absence of cells, the Ti resistance diminishes over time (7 days), due to the presence of amino acids and proteins from the culture medium that have been a dsorbed, while in the presence of osteoblasts, this decrease is much greater because of the compounds generated by the cells that accelerate the dissolution of Ti. RESUMEN: Adhesión de osteoblastos sobre una superficie de Ti de rugosidad controlada.En este trabajo, se ha estudiado la interacción in situ entre células osteoblásticas Saos-2 y una superficie de Ti de rugosidad controlada a lo largo del tiempo. El estudio de la cinética y los mecanismos de proliferación celular de adhesión se ha realizado a través de la microbalanza de cristal de cuarzo (QCM) y espectroscopía de impedancia electroquímica (EIS). La velocidad de adhesión de los osteoblastos sobre la superficie de Ti obtenida a través de medidas con la QCM, sigue una reacción de primer orden, con k=2×10 −3 min −1. Los ensayos de impedancia indican que, en ausencia de las células, la resistencia del Ti disminuye con el tiempo (7 días), debido a la presencia de aminoáci-dos y proteínas del medio de cultivo que se han adsorbido, mientras que en presencia de células, esta disminución es mucho mayor debido a los productos metabólicos generados por las células que aceleran la disolución del Ti.

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Section: Resultssupporting

confidence: 54%

Cell adhesion on Ti surface with controlled roughness

et al. 2015

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“…The decay of the signal may be influenced by adhesion of bacteria to the surface. However, the frequency shift is related to mass loading on the crystal surface, but when monitoring is done in liquid, the frequency also depends on the viscosity and density of the liquid in contact with the resonator (25,40) and is not necessarily directly proportional to the change in ƒ (3,7,11,19,29,37). The frequency shift is proportional to the attached mass only when the mass is rigid and tightly coupled to the surface (20), which is not the case for bacterial cells.…”

Section: Resultsmentioning

confidence: 99%

Use of a Quartz Crystal Microbalance To Investigate the Antiadhesive Potential of N -Acetyl- l -Cysteine

Olofsson

Hermansson

Elwing

2005

Appl Environ Microbiol

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The reduction of bacterial biofilm formation on stainless steel surfaces by N-acetyl-L-cysteine (NAC) is attributed to effects on bacterial growth and polysaccharide production, as well as an increase in the wettability of steel surfaces. In this report, we show that NAC-coated stainless steel and polystyrene surfaces affect both the initial adhesion of Bacillus cereus and Bacillus subtilis and the viscoelastic properties of the interaction between the adhered bacteria and the surface. A quartz crystal microbalance with dissipation was shown to be a powerful and sensitive technique for investigating changes in the applied NAC coating for initial cell surface interactions of bacteria. The kinetics of frequency and dissipation shifts were dependent on the bacteria, the life cycle stage of the bacteria, and the surface. We found that exponentially grown cells gave rise to a positive frequency shift as long as their cell surface hydrophobicity was zero. Furthermore, when the characteristics of binding between the cell and the surface for different growth phases were compared, the rigidity increased from exponentially grown cells to starved cells. There was a trend in which an increase in the viscoelastic properties of the interaction, caused by the NAC coating on stainless steel, resulted in a reduction in irreversibly adhered cells. Interestingly, for B. cereus that adhered to polystyrene, the viscoelastic properties decreased, while there was a reduction in adhered cells, regardless of the life cycle stage. Altogether, NAC coating on surfaces was often effective and could both decrease the initial adhesion and increase the detachment of adhered cells and spores. The most effective reduction was found for B. cereus spores, for which the decrease was caused by a combination of these two parameters.Bacteria commonly adhere to a surface and are part of a complex biofilm. Since the growth of biofilms causes enormous economic losses and health problems in industrial systems like food and paper production, huge efforts are made to solve this problem. The adhesion of bacteria to surfaces is a complex interplay of physical, chemical, and biological factors (5), and there are large variations in the adhesion behaviors of different bacteria and also for different life cycle stages of one strain (41). It is very important to consider these differences when antiadhesion coatings are developed. It is generally thought that bacterial adhesion is preceded by the adsorption of a conditioning layer of molecules that influence adhesion of bacteria to surfaces (43). A good antiadhesion coating should form a conditioning film that prevents adhesion and has a low cohesive strength, making a weak bond between the biofilm and the surface (6). One such agent may be N-acetyl-L-cysteine (NAC). NAC is commonly used in medical treatment of chronic bronchitis and cancer (38,46) and is one of the smallest drug molecules in use (30). Moreover, we recently demonstrated that NAC affects several processes that are important for bacterial biofilm fo...

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“…Conversely, the removal of material from surfaces can be monitored [155][156][157]. Monitoring the rate of surface accumulation of particulates, including cells [158] and atmospheric [159,160] and cosmic dust [161], has also been demonstrated.…”

Section: Materials Deposition and Removalmentioning

confidence: 99%