Osteoblast Differentiation of Amniotic Fluid-Derived Stem Cells Irradiated with Visible Light

Abstract

The effect of visible light irradiation on the expression of pluripotent genes (Oct-4, Sox2, and Nanog) in amniotic fluid-derived stem cells (AFSCs) and on the osteogenic differentiation ability of AFSCs was investigated using light-emitting diodes (LEDs) at 0-2 mW/cm(2) in various wavelengths: [blue (470 nm), green (525 nm), yellow (600 nm), and red (630 nm)]. Pluripotent gene expression in AFSCs was up-regulated by visible light irradiation from a LED for more than 6 h. Green light irradiation of AFSCs up-regulated the expression of pluripotent genes more significantly than irradiation with other light. The osteogenic differentiation of AFSCs was facilitated by green and blue light irradiation. Facilitated differentiation into osteogenic cells by visible light irradiation was not mediated by reactive oxygen species (ROS); alkaline phosphatase activity (a marker of early osteogenic differentiation) and gene expression of osteopontin (a marker of late osteogenic differentiation) did not change significantly between AFSCs in differentiation medium with or without a ROS scavenger (vitamin C). The mitogen-activated protein kinase/extracellular signal-regulated protein kinase pathway, as well as other unknown signaling pathways, may be responsible for the activation of signaling pathways that facilitate the differentiation of AFSCs into osteogenic cells on light irradiation.

Effects of light-emitting diode irradiation on the osteogenesis of human umbilical cord mesenchymal stem cells in vitro

“…Several genes related to antioxidation and mitochondrial energy metabolism are up-regulated or down-regulated in response to light irradiation19. The MAPK/ERK pathway is likely responsible for the activation of signaling pathways resulting in the differentiation of ASFCs into osteogenic cells upon light irradiation20.…”

“…Several studies have investigated the effects of LEDs of various wavelengths on the osteogenic differentiation of amniotic fluid-derived stem cells (ASFCs). The osteogenic differentiation of ASFCs is facilitated by green (525 nm) and blue (470 nm) light irradiation7. Additionally, red LED light promotes the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in osteogenic differentiation medium (ODM)8 and increases the proliferation of osteoblast-like MC3TC-E1 cells9.…”

A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues

“…PCR amplification was performed using Taq DNA polymerase (TITANIUM TM Taq PCR Kit, Clontech Laboratories, Inc., Madison, WI) and a thermocycler (TPersonal, Biometra, GmbH, Goettingen, Germany). Each sample ( n = 3) was tested in duplicate, and the expression level of the GAPDH housekeeping gene was used as a control to normalize the results 28 .…”

Physical cues of cell culture materials lead the direction of differentiation lineages of pluripotent stem cells

“…1). 15,16 Currently, no review articles have specifically addressed the physical cues that cell culture polymeric biomaterials provide for the differentiation of hESCs and hiPSCs, although there are several excellent original and review articles on the differentiation of adult stem cells and mouse ESCs on these biomaterials. 7,9,10,13,17 The differentiation strategies and protocols used with hESCs and hiPSCs are completely different from those used with adult stem cells due to the much higher pluripotency of hPSCs (hESCs and hiPSCs).…”