Osteoblast differentiation of bone marrow stromal cells by femtosecond laser bone ablation

Zheng, Jukuan; Zhang, Xinyue; Zhang, Yaopeng; Yuan, Fusong

doi:10.1364/boe.383721

Cited by 13 publications

(6 citation statements)

References 22 publications

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“…28-30 OCN represents an osteogenic marker protein 31 with its expression, when upregulated, shown to be a useful aid in relation to the promotion of osteoblast differentiation. 32 As a proliferation marker, PCNA and its elevated expression has been highlighted as a hallmark of accelerated proliferation of osteoblasts in osteoporosis rats. 33 The aforementioned evidence highlights the capability of miR-136-5p to promote osteogenic proliferation and differentiation.…”

Section: Discussionmentioning

confidence: 99%

microRNA-136-5p from bone marrow mesenchymal stem cell-derived exosomes facilitates fracture healing by targeting LRP4 to activate the Wnt/β-catenin pathway

Zhang

Liu

2021

Bone & Joint Research

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Aims Exosomes derived from bone marrow mesenchymal stem cells (BMSCs) have been reported to be a promising cellular therapeutic approach for various human diseases. The current study aimed to investigate the mechanism of BMSC-derived exosomes carrying microRNA (miR)-136-5p in fracture healing. Methods A mouse fracture model was initially established by surgical means. Exosomes were isolated from BMSCs from mice. The endocytosis of the mouse osteoblast MC3T3-E1 cell line was analyzed. CCK-8 and disodium phenyl phosphate microplate methods were employed to detect cell proliferation and alkaline phosphatase (ALP) activity, respectively. The binding of miR-136-5p to low-density lipoprotein receptor related protein 4 (LRP4) was analyzed by dual luciferase reporter gene assay. HE staining, tartrate-resistant acid phosphatase (TRAP) staining, and immunohistochemistry were performed to evaluate the healing of the bone tissue ends, the positive number of osteoclasts, and the positive expression of β-catenin protein, respectively. Results miR-136-5p promoted fracture healing and osteoblast proliferation and differentiation. BMSC-derived exosomes exhibited an enriched miR-136-5p level, and were internalized by MC3T3-E1 cells. LRP4 was identified as a downstream target gene of miR-136-5p. Moreover, miR-136-5p or exosomes isolated from BMSCs (BMSC-Exos) containing miR-136-5p activated the Wnt/β-catenin pathway through the inhibition of LRP4 expression. Furthermore, BMSC-derived exosomes carrying miR-136-5p promoted osteoblast proliferation and differentiation, thereby promoting fracture healing. Conclusion BMSC-derived exosomes carrying miR-136-5p inhibited LRP4 and activated the Wnt/β-catenin pathway, thus facilitating fracture healing. Cite this article: Bone Joint Res 2021;10(12):744–758.

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Section: Discussionmentioning

confidence: 99%

microRNA-136-5p from bone marrow mesenchymal stem cell-derived exosomes facilitates fracture healing by targeting LRP4 to activate the Wnt/β-catenin pathway

Zhang

Liu

2021

Bone & Joint Research

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“…On the other hand, studies have revealed that excess ROS could inhibit the osteoinductive effects by interfering with several signaling pathways such as the Wnt signaling and Hedgehog signaling systems [27]. In this study, we further probed the hMSCs with DCFDA to evaluate cellular ROS responses before and after photo‐stimulation.…”

Section: Resultsmentioning

confidence: 99%

“…In this connection, we proposed a differentiation strategy through the pure use of fsL stimulation. Several studies have found that the fsL ablation can promote mouse tibia and craniofacial skeleton regeneration [27, 28]. However, the effects of the direct interaction between fsL and stem cells have rarely been reported.…”

Section: Introductionmentioning

confidence: 99%

A high spatial resolution osteogenic differentiation in human mesenchymal stem cells induced by femtosecond laser

Liu

Chen

Xie

et al. 2022

Journal of Biophotonics

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A variety of physical and chemical methods have been developed in research laboratories for the induction of stem cell differentiation. However, the use of exogenous chemicals and materials may limit their widespread utility in clinics. To develop a clean and precise induction approach with minimal invasion, we reported here that 1‐second stimulation by a tightly focused femtosecond laser (fsL) (140 mW/μm2, 200 fs) can modulate the signaling systems in human mesenchymal cells, such as intracellular calcium and reactive oxygen species. Upon stimulation on an automatic platform, hMSCs were found to express osteoblastic markers and form calcium‐rich deposits. Moreover, tissue mineralization was observed when the fsL‐illuminated hMSCs were ectopically transplanted into nude mice. Collectively, we described a novel and non‐contact optical stimulation method for cell differentiation with high spatiotemporal resolution.

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“…The process of fracture healing requires the production of a large number of new osteoblasts, which accelerates bone synthesis and calcification, and increases bone volume and density [ 27 ]. As the body ages, the number of ABMSCs functional cells decreases, and their potential to differentiate into osteoblasts in the bone marrow is down-regulated [ 28 , 29 ]. Meanwhile, senescence-related secretory phenotypic molecules act in the bone microenvironment and inhibit the osteogenic differentiation of ABMSCs.…”

Section: Discussionmentioning

confidence: 99%

Human umbilical cord mesenchymal stromal cells promotes the proliferation and osteogenic differentiation of autologous bone marrow stem cells by secreting exosomes

Yao

Cao

2022

Bioengineered

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Fractures are frequently encountered diseases troubling the senior population, and the research on fracture repair and the exploration of effective treatment methods are of great significance. This study aimed to clarify the effect of human umbilical cord mesenchymal stromal cell-derived extracellular vesicles (hUMSC-EVs) on the proliferation and osteogenic differentiation of autologous bone marrow stem cells (ABMSCs). The two kinds of cells were co-cultured firstly, 5-Ethynyl-2’- deoxyuridine (EDU) staining and alizarin red staining were used to detect the proliferation and osteogenic differentiation of ABMSCs. The exosomes of hUMSCs were subsequently extracted to process ABMSCs to further test the effect on the cells. The EDU positive rate of ABMSCs and Collagen II expression were elevated, whereas the TdT-mediated dUTP nick end labeling (TUNEL) positive rate and Matrix Metallopeptidase 13 (MMP13) were markedly decreased after the co-culture of hUMSCs and ABMSCs using Transwell chamber assays. The results indicated that hUMSCs could increase the proliferation of ABMSCs, reduce apoptosis, and promote matrix metabolism. The hUMSCs exosomes were separated and added to ABMSCs. As the exosomes content increased, the proliferation of ABMSCs increased simultaneously, and ABMSCs apoptosis decreased. Meanwhile, ABMSCs that migrated to the submembrane increased compared with untreated ABMSCs. Western blot, qPCR and immunofluorescence results revealed that increased exosomes contents promoted the expression of ABMSCs anabolic-related indicators gradually, while decreased the expression of catabolism-related indicators gradually. The previously described results indicated that hUMSCs promoted the proliferation and osteogenic differentiation of ABMSCs by secreting exosomes.

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Osteoblast differentiation of bone marrow stromal cells by femtosecond laser bone ablation

Cited by 13 publications

References 22 publications

microRNA-136-5p from bone marrow mesenchymal stem cell-derived exosomes facilitates fracture healing by targeting LRP4 to activate the Wnt/β-catenin pathway

microRNA-136-5p from bone marrow mesenchymal stem cell-derived exosomes facilitates fracture healing by targeting LRP4 to activate the Wnt/β-catenin pathway

A high spatial resolution osteogenic differentiation in human mesenchymal stem cells induced by femtosecond laser

Human umbilical cord mesenchymal stromal cells promotes the proliferation and osteogenic differentiation of autologous bone marrow stem cells by secreting exosomes

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