Osteoblast-like Cell Proliferation, ALP Activity and Photocatalytic Activity on Sintered Anatase and Rutile Titanium Dioxide

Abstract

This study aimed to create a biomaterial from titanium dioxide (TiO2), which has been known to have photocatalytic and bone formation promoting effects. I expected that anatase titanium dioxide-based implants could promote bone augmentation and induce bone formation. Powdery anatase TiO2 was compression molded and sintered at 700, 800, 900, and 1000 °C to prepare sintered compact samples. X-ray diffraction and scanning electron microscopy were used to observe the surface of these samples. Furthermore, mouse osteoblast-like cells (MC3T3-E1 cell line) were seeded on the samples sintered at different temperatures, and cell proliferation was observed to evaluate the cell proliferation of the samples. The sample sintered at 700 °C was composed of anatase TiO2. The samples sintered at 800 °C and 900 °C were confirmed to consist of a mixture of anatase and rutile TiO2 crystalline phases. Moreover, the sample sintered at 700 and 800 °C, which contained anatase TiO2, showed remarkable photocatalytic activity. Those samples sintered at 1000 °C were transformed to the rutile TiO2. The cell proliferation after 7–14-days culturing revealed that cells cultured on the 700 °C sample decreased in number immediately after initiation of culturing. The cells cultured on TiO2 sintered at 900 °C markedly proliferated over time with an increase in the alkaline phosphatase activity, showing good MC3T3-E1 cell compatibility of the samples. The sample sintered at 1000 °C, which is rutile TiO2, showed the highest increase.

Synergistic Effect of Nano Strontium Titanate Coating and Ultraviolet C Photofunctionalization on Osteogenic Performance and Soft Tissue Sealing of poly(ether–ether–ketone)

“…Meanwhile, to give full play to the surface modification effect of UV functionalization technology, some semiconductor materials can be used as photocatalysts to improve the irradiation efficiency of UV light . The principle is that under UV irradiation, electrons on the valence band can leap to the conduction band to form photogenerated electrons and holes on the valence band, and then the photogenerated electrons and holes react with H 2 O and O 2 adsorbed on the surface of photocatalyst to generate hydroxyl radicals, radicals, and other active groups.…”

Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study

“…However, the effect of these wear particles on the osteoblasts based on their concentration, size, and morphology is not well demonstrated. Studies have shown that TiO 2 nanoparticles have varying effects on osteoblasts and osteoblast-like cells [ 10 , 11 ]. Osteogenic inhibition and bone loss induced by the wear of titanium alloy are crucial in the instigation of peri-prosthetic osteolysis [ 12 ].…”