Osteoblasten

Hofmann, Alexander; Mattyasovszky, Stefan G.; Brüning, Christoph; Ritz, Ulrike; Mehling, Isabella; Meurer, Andrea; Rommens, Pol Maria

doi:10.1007/s00132-009-1488-5

Cited by 9 publications

(3 citation statements)

References 54 publications

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“…Wnt signaling also regulates the expression of Runx2, an osteogenic transcription factor. The activation of the Wnt signaling pathway strongly accelerates osteogenesis [ 22 , 52 ]. In diabetic rats, Wnt signaling plays a central role in diabetic wound healing.…”

Section: Discussionmentioning

confidence: 99%

High-Fat Diet/Low-Dose Streptozotocin-Induced Type 2 Diabetes in Rats Impacts Osteogenesis and Wnt Signaling in Bone Marrow Stromal Cells

Qian

Zhu

et al. 2015

PLoS ONE

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Bone regeneration disorders are a significant problem in patients with type 2 diabetes mellitus. Bone marrow stromal cells (BMSCs) are recognized as ideal seed cells for tissue engineering because they can stimulate osteogenesis during bone regeneration. Therefore, the aim of this study was to investigate the osteogenic potential of BMSCs derived from type 2 diabetic rats and the pathogenic characteristics of dysfunctional BMSCs that affect osteogenesis. BMSCs were isolated from normal and high-fat diet+streptozotocin-induced type 2 diabetic rats. Cell metabolic activity, alkaline phosphatase (ALP) activity, mineralization and osteogenic gene expression were reduced in the type 2 diabetic rat BMSCs. The expression levels of Wnt signaling genes, such as β-catenin, cyclin D1 and c-myc, were also significantly decreased in the type 2 diabetic rat BMSCs, but the expression of GSK3β remained unchanged. The derived BMSCs were cultured on calcium phosphate cement (CPC) scaffolds and placed subcutaneously into nude mice for eight weeks; they were detected at a low level in newly formed bone. The osteogenic potential of the type 2 diabetic rat BMSCs was not impaired by the culture environment, but it was impaired by inhibition of the Wnt signaling pathway, likely due to an insufficient accumulation of β-catenin rather than because of GSK3β stimulation. Using BMSCs derived from diabetic subjects could offer an alternative method of regenerating bone together with the use of supplementary growth factors to stimulate the Wnt signaling pathway.

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Section: Discussionmentioning

confidence: 99%

High-Fat Diet/Low-Dose Streptozotocin-Induced Type 2 Diabetes in Rats Impacts Osteogenesis and Wnt Signaling in Bone Marrow Stromal Cells

Qian

Zhu

et al. 2015

PLoS ONE

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“…We believe that this downward trend is related to fracture healing and bone callus formation. When fracture occurs, the local injury stimulates chemotaxis and growth factor release, resulting in the rapid formation of numerous osteoblasts18. Osteogenic growth factors, mainly secreted by osteoblasts19, also reach peak concentrations at early time points202122.…”

Section: Discussionmentioning

confidence: 99%

The osteogenic potential of human bone callus

Han

Yang

et al. 2016

Sci Rep

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Bone callus, generated during fracture healing, is commonly discarded during surgical procedures. The aim of this study was to investigate the osteogenic potential of bone callus and its possible use as autograft material for patients needing bone grafts. Histology, immunohistochemistry, micro-computed tomography, and biomechanics were performed to examine osteogenic cells, osteoinductive factors, and the osteoconductive structure of bone callus. Alkaline phosphatase-positive osteoblasts, osteoinductive factors (including BMP2, FGF2, TGFB1, and IGF1), and a porous structure were found in bone callus. Early-stage callus (within 3 months after fracture) presented significantly improved osteogenic properties compared to medium- (3–9 months) and late-stage (longer than 9 months) callus. The results revealed that bone callus induced new bone formation in a nude mouse model. Early-stage callus showed better performance to medium- and late-stage callus in the induction of new bone formation at both 8 and 12 weeks. These findings indicated that bone callus, especially early-stage callus, possesses osteogenic potential and can potentially serve as an alternative source of material for bone grafts.

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“…However, reprogramming a cell through pathway engineering can also be used for controlled and sustainable differentiation of stem cells with high yields as well as for directing other cellular behaviors such as expression and/or secretion of a specific protein. By this way cells can be manipulated utilizing the knowledge on the signaling pathways for chondrogenic differentiation of MSCs [165], skin aging [166], fracture healing of the bone [167], liver regeneration [168] or cellular apoptosis [169], in order to provide solutions for engineering complex tissues. For example, human pluripotent stem cells were reprogrammed into multipotent neural crest cells through activation of the canonical Wnt signaling and suppression of the Activin A/Nodal pathway simultaneously [170].…”

Section: Cell Sources and Controlling Cell Behaviormentioning

confidence: 99%

The Expanding World of Tissue Engineering: The Building Blocks and New Applications of Tissue Engineered Constructs

Zorlutuna

Vrana

Khademhosseini

2013

IEEE Rev. Biomed. Eng.

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The field of tissue engineering has been growing in the recent years as more products have made it to the market and as new uses for the engineered tissues have emerged, motivating many researchers to engage in this multidisciplinary field of research. Engineered tissues are now not only considered as end products for regenerative medicine, but also have emerged as enabling technologies for other fields of research ranging from drug discovery to biorobotics. This widespread use necessitates a variety of methodologies for production of tissue engineered constructs. In this review, these methods together with their non-clinical applications will be described. First, we will focus on novel materials used in tissue engineering scaffolds; such as recombinant proteins and synthetic, self assembling polypeptides. The recent advances in the modular tissue engineering area will be discussed. Then scaffold-free production methods, based on either cell sheets or cell aggregates will be described. Cell sources used in tissue engineering and new methods that provide improved control over cell behavior such as pathway engineering and biomimetic microenvironments for directing cell differentiation will be discussed. Finally, we will summarize the emerging uses of engineered constructs such as model tissues for drug discovery, cancer research and biorobotics applications.

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Osteoblasten

Cited by 9 publications

References 54 publications

High-Fat Diet/Low-Dose Streptozotocin-Induced Type 2 Diabetes in Rats Impacts Osteogenesis and Wnt Signaling in Bone Marrow Stromal Cells

High-Fat Diet/Low-Dose Streptozotocin-Induced Type 2 Diabetes in Rats Impacts Osteogenesis and Wnt Signaling in Bone Marrow Stromal Cells

The osteogenic potential of human bone callus

The Expanding World of Tissue Engineering: The Building Blocks and New Applications of Tissue Engineered Constructs

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