Osteoblastic proliferative activity of Epimedium brevicornum Maxim.

Meng, Fan‐hao; Li, Y.-B.; Xiong, Zhili; Jiang, Zhiming; Li, F.-M.

doi:10.1016/j.phymed.2004.03.007

Cited by 137 publications

(101 citation statements)

References 5 publications

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“…Besides, our previous study indicated that HETF could stimulate the proliferation and differentiation of primary osteoblasts (Zhang et al, 2008). It is also reported that the crude extract, total flavonoids, and main flavonoid constituents from Herba Epimedii (E. brevicornum Maxim and E. koreanum Nakai) have osteoblastic proliferation-stimulating activity toward osteoblast-like UMR106 cells (Meng et al, 2005;Xie et al, 2005).…”

Section: Discussionmentioning

confidence: 92%

“…Total flavonoids of Herba Epimedii (HETF) were found to suppress urinary calcium excretion and improve bone properties in ovariectomized mice (Chen et al, 2011;Xie et al, 2005). The extracts of Herba Epimedii promoted the proliferation and differentiation of primary osteoblasts (Zhang et al, 2008) and osteoblast-like UMR106 cells (Meng et al, 2005). Our previous study has shown that HETF significantly inhibited the proliferation and differentiation of RAW264.7 cells, suppressed vitamin D-induced differentiation of osteoclasts in rabbit bone marrow cells, and decreased the bone resorption of rabbit mature osteoclasts in vitro (Zhang et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Flavonoids of Herba Epimedii stimulate osteogenic differentiation and suppress adipogenic differentiation of primary mesenchymal stem cells via estrogen receptor pathway

Zhang

Liu

Jia

et al. 2015

Pharmaceutical Biology

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Objective: The present study investigates the effect of total flavonoids of Herba Epimedii (HETF) on the osteogenesis and adipogenesis of primary MSCs. Materials and methods: HETF were prepared and identified by HPLC-fingerprinting, primary mouse MSCs in the presence of 0.006-6 mg/mL HETF for 2-10 d were subject to morphological, biochemical, and quantitative real-time PCR analysis. Results: Sixteen chemical components were identified in HETF by HPLC-fingerprinting and account for over 95% of the total area of HPLC peaks. During osteogenesis of MSCs, 0.006-6 mg/mL HETF promoted the proliferation of MSCs from 17% to 22%, increased alkaline phosphatase activity up to 3.7-fold (0.6 mg/mL), and extracellular calcium deposits from 1.2-to 1.4-folds by up-regulating the expression of runt-related transcription factor-2 (Runx-2) and bone morphogenetic protein-2 (BMP-2). Meanwhile, HETF suppressed the adipogenesis of MSCs by reducing the formation of adipocytelike cells and accumulation of fat droplets by down-regulating the expression of peroxisome proliferator-activated receptor-g (PPAR-g). The above biological activities of HETF were mainly through estrogen receptor-mediated pathway, which were blocked by estrogen receptor antagonist, ICI 182,780. Conclusion: HETF could regulate Runx-2-mediated osteogenesis and PPAR-g-mediated adipogenesis in MSCs and thus exhibit beneficial effects to bone health, which suggests a new strategy for treating patients with osteoporosis and obesity.ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Flavonoids of Herba Epimedii stimulate osteogenic differentiation and suppress adipogenic differentiation of primary mesenchymal stem cells via estrogen receptor pathway

Zhang

Liu

Jia

et al. 2015

Pharmaceutical Biology

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“…Icariin has been found to be the most abundant osteogenic compound in the BuOH and EtOAc fractions of Epimedium plants (Meng et al, 2005;Zhang et al, 2008), so this compound was used to examine the effect of Epimedium brevicornum on estrogen biosynthesis in KGN cells. The chemical structure of icariin is shown in Fig.…”

Section: Effect Of Icariin On Estrogen Biosynthesis In Kgn Cellsmentioning

confidence: 99%

“…Epimedium brevicornum, a traditional Chinese medicine, has been widely used in China for the treatment of cardiovascular diseases, infertility, impotence, amnesia, lumbago, arthritis, and numbness and weakness of the limbs for thousands of years (Ma et al, 2011). Icariin is the main flavonoid present in Epimedium brevicornum, used for herbal quality control (Chinese Pharmacopoeia Commission, 2010), and is considered the major bioactive component of this plant (Meng et al, 2005;Ma et al, 2011). Several studies have shown that both Epimedium brevicornum and icariin have antiosteoporotic effects in vitro and in vivo by stimulating osteoblast proliferation and function; these findings support the wide use of Epimedium brevicornum in many Chinese formulas for treating osteoporosis (Zhang et al, 2007Qin et al, 2008;Nian et al, 2009;Hsieh et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Icariin from Epimedium brevicornum Maxim promotes the biosynthesis of estrogen by aromatase (CYP19)

Yang

Guo

et al. 2013

Journal of Ethnopharmacology

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“…for the treatment of osteoporosis, menopausal syndrome, and age-associated diseases (8). These traditional Chinese medicines containing multiple interactive compounds have attracted the attention of researchers for their effects in the management of menopausal and related medical conditions (9).…”

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confidence: 99%

Effects of Epimedium polysaccharide on female mouse (Mus musculus)ovarian and uterine development

Afedo¹,

Xu²,

Muneri³

et al. 2015

Turk J Vet Anim Sci

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This study was conducted to investigate the effects of Epimedium polysaccharide (EPS) on ovarian and uterine development in female mice and assess the in vitro maturation (IVM) rate. Healthy Kunming female mice were divided into 4 groups and administered 2, 4, 6, and 8 mg/0.1 mL of EPS, respectively; the control group was given 0.1 mL of 0.9% physiological saline solution. After 7 and 14 days, the mice were sacrificed and their reproductive tracts (uterus, oviduct, and ovaries) were removed. The reproductive tract was weighed; some ovaries were punctured to harvest oocytes for IVM culture and the others were used for histological analysis. EPS had virtually no effect on the body weight of the female mice. EPS at 4 mg/0.1 mL for 14 days of treatment had a significant effect (P < 0.05) on ovarian and uterine development when compared with 2, 6, and 8 mg/mL but no significant effect (P > 0.05) when compared with the control group. The number of follicles increased in the experimental groups and the arrangements of ovarian follicles were not negatively affected during histological observation. In conclusion, EPS at appreciable dosages has a positive effect on mouse ovarian development.

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Osteoblastic proliferative activity of Epimedium brevicornum Maxim.

Cited by 137 publications

References 5 publications

Flavonoids of Herba Epimedii stimulate osteogenic differentiation and suppress adipogenic differentiation of primary mesenchymal stem cells via estrogen receptor pathway

Flavonoids of Herba Epimedii stimulate osteogenic differentiation and suppress adipogenic differentiation of primary mesenchymal stem cells via estrogen receptor pathway

Icariin from Epimedium brevicornum Maxim promotes the biosynthesis of estrogen by aromatase (CYP19)

Effects of Epimedium polysaccharide on female mouse (Mus musculus)ovarian and uterine development

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