Osteoblasts interact with MTA surfaces and express Runx2

Perinpanayagam, Hiran; AL-Rabeah, Ebtehal

doi:10.1016/j.tripleo.2008.12.005

Cited by 31 publications

(12 citation statements)

References 26 publications

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“…In this study, we prepared an MTA solution as previously described18 and examined the effect of this MTA solution on osteoclast formation. In some in vitro studies, the test materials were placed in direct contact with the cells 22–24. Pretreated plastic is usually used for optimal cell attachment, and any interruption of the coated surface may inhibit cell attachment, and subsequently cell number and proliferation, because cells require optimal cell‐to‐surface and cell‐to‐cell contact for growth 25.…”

Section: Discussionmentioning

confidence: 99%

“…Three major transcription factors, AP‐1,31 Runx232 and TCF/LEF,33 regulate OPG gene expression. Although it is still unclear which transcription factors affect the 1α,25(OH) 2 D 3 ‐induced downregulation of OPG, MTA might regulate their activation 24. Further studies will be required to determine a more detailed mechanism by which MTA solution selectively blocks the 1α,25(OH) 2 D 3 ‐induced downregulation of OPG expression in POBs.…”

Section: Discussionmentioning

confidence: 99%

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Mineral trioxide aggregate solution inhibits osteoclast differentiation through the maintenance of osteoprotegerin expression in osteoblasts

Hashiguchi

Fukushima

Nakamura

et al. 2010

J Biomedical Materials Res

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Mineral trioxide aggregate (MTA) is a therapeutic, endodontic repair material that is reported to exhibit calcified tissue-conductive activity. The aim of this study was to investigate whether MTA may prevent osteoclast differentiation in vitro. MTA solution, but not other commonly used retrofilling materials, such as Dycal, Super-EBA, or intermediate restorative material (IRM) solution, dose-dependently inhibited osteoclastogenesis in cocultures of mouse bone marrow cells (BMCs) with primary osteoblast cells (POBs) induced by 1α,25-dihydroxyvitamin D(3) [1α,25(OH)(2) D(3) ]. Exogenous CaCl(2) medium supplementation did not inhibit osteoclastogenesis in cocultures. Furthermore, MTA solution did not affect receptor activator of NF-κB ligand (RANKL)-induced osteoclastogenesis, suggesting that POBs are targets of MTA. MTA solution suppressed the 1α,25(OH)(2) D(3) -induced reduction of osteoprotegerin (OPG) mRNA and protein production without changing RANKL expression in POBs. Consistent with this result, MTA solution did not inhibit osteoclastogenesis in cocultures of BMCs and POBs from OPG-deficient mice. Therefore, the maintenance of OPG expression in POBs appears to be critical for the inhibitory effect of MTA solution on osteoclast differentiation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Mineral trioxide aggregate solution inhibits osteoclast differentiation through the maintenance of osteoprotegerin expression in osteoblasts

Hashiguchi

Fukushima

Nakamura

et al. 2010

J Biomedical Materials Res

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“… 56 Tani-Ishii et al 57 reported that, in osteoblasts, faster growth of cells and increased mineralized matrix gene expression has been observed in the presence of MTA. Also, Perinpanayagam & Al-Rabeah 58 have reported that MTA supports cell attachment and expression of Runt-related transcription factor 2 (RUNX2), the osteoblast lineage master transcriptional factor. In addition, some studies have evaluated the use of different titanium alloys combined with implant surfaces bio-modifications to function as stem cell carrier for enhancement of osteointegration.…”

Section: Scaffold Materialsmentioning

confidence: 99%

Dental pulp stem cells

Ashri

Ajlan

Aldahmash

2015

SMJ

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Inflammatory periodontal disease is a major cause of loss of tooth-supporting structures. Novel approaches for regeneration of periodontal apparatus is an area of intensive research. Periodontal tissue engineering implies the use of appropriate regenerative cells, delivered through a suitable scaffold, and guided through signaling molecules. Dental pulp stem cells have been used in an increasing number of studies in dental tissue engineering. Those cells show mesenchymal (stromal) stem cell-like properties including self-renewal and multilineage differentiation potentials, aside from their relative accessibility and pleasant handling properties. The purpose of this article is to review the biological principles of periodontal tissue engineering, along with the challenges facing the development of a consistent and clinically relevant tissue regeneration platform. This article includes an updated review on dental pulp stem cells and their applications in periodontal regeneration, in combination with different scaffolds and growth factors.

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“…Therefore, considerable efforts have focused on studying tissue‐implant interactions, and on enhancing the biocompatibility and osteo‐inductivity of the biomaterial surfaces so as to optimize the cellular response . Studies have shown that various surface additives can enhance cell survival, attachment, metabolism, proliferation, and differentiation, and that surface modifications such as grinding and chemical etching can improve the biocompatibility of implants . Similarly, a novel surface coating technique was developed, whereby the surfaces of titanium implants can be enhanced with a polyester‐based ultrafine dry powder coating.…”

Section: Introductionmentioning

confidence: 99%

Epoxy resin‐based ultrafine dry powder coatings for implants

Hou

Zhu

Zhang

et al. 2016

J of Applied Polymer Sci

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Ultrafine dry powder coating technology creates biocompatible polymeric coatings for implants. Nanoparticles (nTiO 2 ) modify flow to prevent agglomeration and create homogenous coatings. Since polyester-based coatings require the potentially harmful 1,3,5-triglycidyl isocyanurate (TGIC) curing agent, this study's objective was to develop alternative TGIC-free formulations. Epoxy and epoxy/polyester (1:1) hybrid mixtures were enriched with CaO (5% w/w) and nTiO 2 (0.5% w/w), as functional additives and flow modifiers, respectively. Epoxy-TiO 2 and Hybrid-TiO 2 mixtures were prepared with micron-sized TiO 2 (25% w/w) to enhance biocompatibility. Polymer chips and additives were combined in a high-shear mixer and passed through a sieve (35 mm) to yield ultrafine particles that were sprayed (20 kV) onto metal sheets and cured (200 8C). Particle size analyses showed that all formulations were ultrafine (D 0.5 < 35 mm), and epoxy/polyester/TiO 2 mixtures were the smallest (D 0.5 5 16.34 mm). Angles of repose, avalanche and resting indicated reduced flowability when epoxy was enriched with TiO 2 and/or polyester, although all formulae were highly flowable. Elemental mapping of coatings showed a predominance of carbon (C) and oxygen (O) from resin polymer, and elevated titanium (Ti) in the TiO 2 enriched surfaces. However, calcium (Ca) clusters were higher on the epoxy/polyester Hybrid coatings. Optical microscopy showed human mesenchymal cells (ATCC CRL-1486) attached and spread out, and Alizarin Red staining showed mineral deposits in 2-4 week cultures, particularly on epoxy/polyester/TiO 2 Hybrid surfaces. These epoxy resin-based formulations were effective TGIC-free substitutes for ultrafine dry powder coatings on implants. V C 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43960.

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Osteoblasts interact with MTA surfaces and express Runx2

Cited by 31 publications

References 26 publications

Mineral trioxide aggregate solution inhibits osteoclast differentiation through the maintenance of osteoprotegerin expression in osteoblasts

Mineral trioxide aggregate solution inhibits osteoclast differentiation through the maintenance of osteoprotegerin expression in osteoblasts

Dental pulp stem cells

Epoxy resin‐based ultrafine dry powder coatings for implants

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