Osteoclastic resorption of Ca-P biomaterials implanted in rabbit bone

Basle, Michel‐Félix; Chappard, Daniel; Grizon, F.; Filmon, R.; Delécrin, J.; Daculsi, Guy; Rèbel, A

doi:10.1007/bf01351842

Cited by 96 publications

(50 citation statements)

References 37 publications

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“…During this period, the ceramic degraded by a disso- lution-precipitation process, as previously demonstrated by high-resolution transmission electron microscopy (TEM). 21,37,41,45 The dissolution and reprecipitation of biological apatite occurs inside the micropores of the MBCP ceramic. The first cells to arrive-macrophages-are rapidly replaced by osteoblasts and osteoclasts, which produce and remodel bone in the ceramic implant.…”

Section: Discussionmentioning

confidence: 99%

Small‐animal models for testing macroporous ceramic bone substitutes

et al. 2004

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The aim of this study was to compare the bone colonization of a macroporous biphasic calcium phosphate (MBCP) ceramic in different sites (femur, tibia, and calvaria) in two animal species (rats and rabbits). A critical size defect model was used in all cases with implantation for 21 days. Bone colonization in the empty and MBCP-filled defects was measured with the use of backscattered electron microscopy (BSEM). In the empty cavities, bone healing remained on the edges, and did not bridge the critical size defects. Bone growth was observed in all the implantation sites in rats (approx. 13.6 -36.6% of the total defect area, with ceramic ranging from 46.1 to 51.9%). The bone colonization appeared statistically higher in the femur of rabbits (48.5%) than in the tibia (12.6%) and calvaria (22.9%) sites. This slightly higher degree of bone healing was related to differences in the bone architecture of the implantation sites. Concerning the comparison between animal species, bone colonization appeared greater in rabbits than in rats for the femoral site (48.5% vs. 29.6%). For the other two sites (the tibia and calvaria), there was no statistically significant difference. The increased bone ingrowth observed in rabbit femurs might be due to the large bone surface area in contact with the MBCP ceramics. The femoral epiphysis of rabbits is therefore a favorable model for testing the bone-bonding capacity of materials, but a comparison with other implantation sites is subject to bias. This study shows that well-conducted and fully validated models with the use of small animals are essential in the development of new bone substitutes.

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Section: Discussionmentioning

confidence: 99%

Small‐animal models for testing macroporous ceramic bone substitutes

et al. 2004

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“…Because surface reactivity and physical topography can both influence osteoblast differentiation but are linked to surface architecture, it is currently unknown which, if either, material property plays a prevailing role in osteoinduction (Curran et al, 2006;Habibovic et al, 2006a;Vlacic-Zischke et al, 2011;Zhao et al, 2007). Alternatively, osteoinduction may depend on (pre-) osteoclast activity for osteogenic signals rather than intrinsic physicochemical signals originating from the material itself (Baslé et al, 1993;Gauthier et al, 2005;Malard et al, 1999). In support of this theory, it has been reported that osteoclastogenesis precedes osteoinduction by microstructured TCP by several weeks (Akiyama et al, 2011;Kondo et al, 2006), and osteoclast depletion limits (Ripamonti et al, 2010) or completely blocks de novo bone formation by osteoinductive CaPs (Davison et al, 2014a).…”

Section: Nl Davison Et Al Osteoinduction and Osteoclastogenesis By Bmentioning

confidence: 99%

Influence of surface microstructure and chemistry on osteoinduction and osteoclastogenesis by biphasic calcium phosphate discs

Davison

Su²,

Yuan³

et al. 2015

eCM

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It has been reported that surface microstructural dimensions can influence the osteoinductivity of calcium phosphates (CaPs), and osteoclasts may play a role in this process. We hypothesised that surface structural dimensions of ≤ 1 μm trigger osteoinduction and osteoclast formation irrespective of macrostructure (e.g., concavities, interconnected macropores, interparticle space) or surface chemistry. To test this, planar discs made of biphasic calcium phosphate (BCP: 80 % hydroxyapatite, 20 % tricalcium phosphate) were prepared with different surface structural dimensions -either ~ 1 μm (BCP1150) or ~ 2-4 μm (BCP1300) -and no macropores or concavities. A third material was made by sputter coating BCP1150 with titanium (BCP1150Ti), thereby changing its surface chemistry but preserving its surface structure and chemical reactivity. After intramuscular implantation in 5 dogs for 12 weeks, BCP1150 formed ectopic bone in 4 out of 5 samples, BCP1150Ti formed ectopic bone in 3 out of 5 samples, and BCP1300 formed no ectopic bone in any of the 5 samples. In vivo, large multinucleated osteoclast-like cells densely colonised BCP1150, smaller osteoclast-like cells formed on BCP1150Ti, and osteoclast-like cells scarcely formed on BCP1300. In vitro, RAW264.7 cells cultured on the surface of BCP1150 and BCP1150Ti in the presence of osteoclast differentiation factor RANKL (receptor activator for NF-κB ligand) proliferated then differentiated into multinucleated osteoclast-like cells with positive tartrate resistant acid phosphatase (TRAP) activity. However, cell proliferation, fusion, and TRAP activity were all significantly inhibited on BCP1300. These results indicate that of the material parameters tested -namely, surface microstructure, macrostructure, and surface chemistry -microstructural dimensions are critical in promoting osteoclastogenesis and triggering ectopic bone formation.

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“…Intensive efforts have been made to determine the most adequate composition and architecture. On the chemical side, many materials varying in composition and architecture have been proposed, including polymers (Ishaug-Riley et al, 1997;Ignatius et al, 2001;Mondrinos et al, 2006;Gogolewski et al, 2008), metals (Ayers et al, 1999;Bobyn et al, 1999;Itala et al, 2001;Witte et al, 2007) and ceramics (Klawitter and Hulbert, 1971;Klein et al, 1985;van Blitterswijk et al, 1986;Eggli et al, 1988;Daculsi and Passuti, 1990;Schliephake et al, 1991;Basle et al, 1993;Metsger et al, 1993;Lu et al, 1999;Flautre et al, 2001;Walsh et al, 2003;Jones and Hench, 2004;Linhart et al, 2004;Hench, 2006;Von Doernberg et al, 2006;Lan Levengood et al, 2010;Murakami et al, 2010;Yuan et al, 2010;Polak et al, 2011;Haugen et al, 2013). These materials present very different resorption rates, and many resorption mechanisms, such as dissolution, hydrolysis (e.g., poly(α-hydroxy acids) (Ignatius et al, 2001)), cell-mediated resorption (Basle et al, 1993;Lu et al, 1999;Von Doernberg et al, 2006;Yuan et al, 2010), corrosion (Witte et al, 2007), enzymatic degradation (Hutmacher, 2000;Vert, 2007), and transport <...>…”

Section: Introductionmentioning

confidence: 99%

Effect of grain size and microporosity on the in vivo behaviour of β-tricalcium phosphate scaffolds

Lapczyna

Galea²,

Wüst

et al. 2014

eCM

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Defining the most adequate architecture of a bone substitute scaffold is a topic that has received much attention over the last 40 years. However, contradictory results exist on the effect of grain size and microporosity. Therefore, the aim of this study was to determine the effect of these two factors on the in vivo behaviour of β-tricalcium phosphate (β-TCP) scaffolds. For that purpose, β-TCP scaffolds were produced with roughly the same macropore size (≈ 150 μm), and porosity (≈ 80 %), but two levels of microporosity (low: 10 % / high: ≈ 25 %) and grain size (small: 1.3 μm /large: ≈ 3.3 μm). The sample architecture was characterised extensively using materialography, Hg porosimetry, micro-computed tomography (μCT), and nitrogen adsorption. The scaffolds were implanted for 2, 4 and 8 weeks in a cylindrical 5-wall cancellous bone defect in sheep. The histological, histomorphometrical and μCT analysis of the samples revealed that all four scaffold types were almost completely resorbed within 8 weeks and replaced by new bone. Despite the three-fold difference in microporosity and grain size, very few biological differences were observed. The only significant effect at p < 0.01 was a slightly faster resorption rate and soft tissue formation between 4 and 8 weeks of implantation when microporosity was increased. Past and present results suggest that the biological response of this particular defect is not very sensitive towards physico-chemical differences of resorbable bone graft substitutes. As bone formed not only in the macropores but also in the micropores, a closer study at the microscopic and localised effects is necessary.

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Osteoclastic resorption of Ca-P biomaterials implanted in rabbit bone

Cited by 96 publications

References 37 publications

Small‐animal models for testing macroporous ceramic bone substitutes

Small‐animal models for testing macroporous ceramic bone substitutes

Influence of surface microstructure and chemistry on osteoinduction and osteoclastogenesis by biphasic calcium phosphate discs

Effect of grain size and microporosity on the in vivo behaviour of β-tricalcium phosphate scaffolds

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