2020
DOI: 10.21203/rs.3.rs-24807/v1
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Osteogenic and Fluorescent Characterization of Pig-Derived Mesenchymal Stem Cells Between eGFP Transgenesis and Non-viral Plasmid Transfection

Abstract: BackgroundWidely used in recent years, mesenchymal stem cells (MSCs) expressing enhanced green fluorescent protein (eGFP) can be tracked during migration to injury sites, while also supporting tri-lineage differentiation. However, the relationship between the expression of green fluorescence and the magnitude of osteogenic differentiation is not clearly defined. Despite increasing use of eGFP-MSCs derived from the transgenic pigs and non-viral eGFP plasmid transfected MSCs in recent years, it remains unclear w… Show more

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