Osteogenic differentiation and angiogenesis with cocultured adipose-derived stromal cells and bone marrow stromal cells

Kim, Kyung-Il; Park, Siyeon; Im, Gun‐Il

doi:10.1016/j.biomaterials.2014.02.048

Cited by 86 publications

(71 citation statements)

References 33 publications

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“…Furthermore, we could demonstrate that conditioned media harvested from osteoporotic patient-derived ASCs was able to better support osteoporotic patient-derived BMSC osteogenesis. Although similar findings were reported in studies utilizing human ASCs with either human BMSCs or mouse osteoblastic cell lines [21,39], as far as we are aware, this is the first direct evidence that human osteoporotic ASCs have the capacity to impart a pro-osteogenic paracrine effect on human osteoporotic patientderived BMSCs in vitro. It is possible therefore, that the increases in SAMP6 trabecular bone quality following ASC treatment were due, in part, to the paracrine actions of injected ASCs on resident BMSC populations.…”

Section: Discussionmentioning

confidence: 51%

Therapeutic potential of adipose-derived stromal cells in age-related osteoporosis

et al. 2014

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a b s t r a c tAdipose-derived stromal cells (ASCs) are increasingly being used for orthopedic-based tissue engineering approaches due to their ability to readily undergo osteogenic differentiation. In the present study, we used in vitro and in vivo approaches to evaluate the use of ASCs as a treatment strategy for age-related osteoporosis. Molecular, histological and micro-computed tomography (micro-CT) based approaches confirmed that ASCs isolated from 18-week-old osteoporotic senescence-accelerated mice (SAMP6) were capable of undergoing osteogenesis when cultured in either silk fibroin (SF) scaffolds or scaffold-free microtissues (ASC-MT). A single intratibial injection of CM-Dil-labeled isogeneic ASCs or ASC-MT into SAMP6 recipients significantly improved trabecular bone quality after 6 weeks in comparison to untreated contralateral bones, as determined by micro-CT. Injected ASCs could be observed in paraffin wax bone sections at 24 h and 6 weeks post treatment and induced a significant increase in several molecular markers of bone turnover. Furthermore, a significant improvement in the osteogenic potential of osteoporotic patient-derived human bone marrow stromal cells (BMSCs) was observed when differentiated in conditioned culture media harvested from osteoporotic patient-derived human ASCs. These findings therefore support the use of ASCs as an autologous cell-based approach for the treatment of osteoporosis.

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Section: Discussionmentioning

confidence: 51%

Therapeutic potential of adipose-derived stromal cells in age-related osteoporosis

et al. 2014

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“…The most frequently used materials in ASCs tissue regeneration include collagen [14], hyaluronic acid [20], and polylactic-co-glycolic acid [21]. In this respect, different clinical trials are enrolling patients for the regeneration of craniofacial, cardiovascular and soft tissues [22].…”

Section: Introductionmentioning

confidence: 99%

Hybrid Complexes of High and Low Molecular Weight Hyaluronans Highly Enhance HASCs Differentiation: Implication for Facial Bioremodelling

Stellavato

Noce

Corsuto

et al. 2017

Cell Physiol Biochem

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Background/Aims: Adipose-derived Stem Cells (ASCs) are used in Regenerative Medicine, including fat grafting, recovery from local tissue ischemia and scar remodeling. The aim of this study was to evaluate hyaluronan based gel effects on ASCs differentiation and proliferation. Methods: Comparative analyses using high (H) and low (L) molecular weight hyaluronans (HA), hyaluronan hybrid cooperative complexes (HCCs), and high and medium cross-linked hyaluronan based dermal fillers were performed. Human ASCs were characterized by flow cytometry using CD90, CD34, CD105, CD29, CD31, CD45 and CD14 markers. Then, cells were treated for 7, 14 and 21 days with hyaluronans. Adipogenic differentiation was evaluated using Oil red-O staining and expression of leptin, PPAR-γ, LPL and adiponectin using qRT-PCR. Adiponectin was analyzed by immunofluorescence, PPAR-γ and adiponectin were analyzed using western blotting. ELISA assays for adiponectin and leptin were performed. Results: HCCs highly affected ASCs differentiation by up-regulating adipogenic genes and related proteins, that were also secreted in the culture medium. H-HA and L-HA induced a lower level of ASCs differentiation. Conclusion: HCCs-based formulations clearly enhance adipogenic differentiation and proliferation, when compared with linear HA and cross-linked hyaluronans. Injection of HCCs in subdermal fat compartment may recruit and differentiate stem cells in adipocytes, and considerably improving fat tissue renewal.

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“…We found that epithelial cells co-cultured with SVF cells exhibited enhanced anti-inflammatory and pro-angiogenetic responses under oxidative stress conditions [24], and MSCs co-cultivated with SVF cells ameliorated the apoptosis induced by oxidative stress. Co-culture is a useful and powerful tool to understand cellular interactions and paracrine mechanisms [33], and this method can present a more comprehensive signal for differentiation to a certain lineage; however, it is very difficult to standardize in primary cells due to variation between individual donors [33]. We wondered whether SVF cells also induce the differentiation of MSCs, which are multipotent stromal cells that can differentiate into a variety of cell types.…”

Section: Discussionmentioning

confidence: 99%

“…2 and 3). Because this period is very short compared with the typical time required for the in vitro osteogenic differentiation of MSCs [33-35], we could not identify a proper positive osteogenic control and used only negative controls such as mono-cultured MSCs or SVF cells in all experiments.…”

Section: Discussionmentioning

confidence: 99%

Rapid Induction of Osteogenic Markers in Mesenchymal Stem Cells by Adipose-Derived Stromal Vascular Fraction Cells

Choi

Shin

Lee

et al. 2017

Cell Physiol Biochem

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Background/Aims: Stromal vascular fraction (SVF) cells are a mixed cell population, and their regenerative capacity has been validated in various therapeutic models. The purpose of this study was to investigate the regenerative mechanisms utilized by implanted SVF cells. Using an in vitro co-culture system, we sought to determine whether SVF implantation into impaired tissue affects endogenous mesenchymal stem cell (MSC) differentiation; MSCs can differentiate into a variety of cell types, and they have a strong regenerative capacity despite their low numbers in impaired tissue. Methods: Adipose-derived SVF cells obtained from four donors were co-cultured with bone marrow-derived MSCs, and the differential expression of osteogenic markers and osteogenic differentiation inducers over time was analyzed in mono-cultured MSCs and MSCs co-cultured with SVF cells. Results: The co-cultivation of MSCs with SVF cells significantly and mutually induced the expression of osteogenic-specific markers via paracrine and/or autocrine regulation but did not induce adipocyte, chondrocyte or myoblast marker expression. More surprisingly, subsequent osteogenesis and/or comparable effects were rapidly induced within 48 h. Conclusion: To the best of our knowledge, this is the first study in which osteogenesis and/or comparable effects were rapidly induced in bone marrow-derived MSCs and adipose-derived SVF cells through co-cultivation. Our findings suggest that the positive effects of SVF implantation into impaired bone may be attributed to the rapid induction of MSC osteogenesis, and the transplantation of co-cultured and preconditioned SVF cells and/or MSCs may be more effective than the transplantation of untreated cells for the treatment of bone defects.

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Osteogenic differentiation and angiogenesis with cocultured adipose-derived stromal cells and bone marrow stromal cells

Cited by 86 publications

References 33 publications

Therapeutic potential of adipose-derived stromal cells in age-related osteoporosis

Therapeutic potential of adipose-derived stromal cells in age-related osteoporosis

Hybrid Complexes of High and Low Molecular Weight Hyaluronans Highly Enhance HASCs Differentiation: Implication for Facial Bioremodelling

Rapid Induction of Osteogenic Markers in Mesenchymal Stem Cells by Adipose-Derived Stromal Vascular Fraction Cells

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