Osteogenic differentiation of electrostimulated human mesenchymal stem cells seeded on silk-fibroin films

Çakmak, Anıl Sera; Çakmak, Soner; White, James D.; Raja, Waseem; Kaplan, David L.; Gümüşderelı́oğlu, Menemşe

doi:10.3906/biy-1505-114

Cited by 2 publications

(1 citation statement)

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“…Landmark mechanistic studies by Nelson et al identified Ca 2+ signaling as a key pathway for the effects of electrical stimulation, and this signal transduction is particular to the specific type of physical stimulus applied [ 13 ]. Most in vitro studies conclude that electric fields can be used as biophysical stimulants, and that responses can be selected by manipulating parameters such as frequency, voltage, composition of the cell culture medium or nature of the field applied [ 14 – 15 ].…”

Section: Introductionmentioning

confidence: 99%

Blood Stage Plasmodium falciparum Exhibits Biological Responses to Direct Current Electric Fields

et al. 2016

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The development of resistance to insecticides by the vector of malaria and the increasingly faster appearance of resistance to antimalarial drugs by the parasite can dangerously hamper efforts to control and eradicate the disease. Alternative ways to treat this disease are urgently needed. Here we evaluate the in vitro effect of direct current (DC) capacitive coupling electrical stimulation on the biology and viability of Plasmodium falciparum. We designed a system that exposes infected erythrocytes to different capacitively coupled electric fields in order to evaluate their effect on P. falciparum. The effect on growth of the parasite, replication of DNA, mitochondrial membrane potential and level of reactive oxygen species after exposure to electric fields demonstrate that the parasite is biologically able to respond to stimuli from DC electric fields involving calcium signaling pathways.

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Section: Introductionmentioning

confidence: 99%

Blood Stage Plasmodium falciparum Exhibits Biological Responses to Direct Current Electric Fields

et al. 2016

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Combining electrical stimulation and tissue engineering to treat large bone defects in a rat model

Leppik

Han

Mobini

et al. 2018

Sci Rep

161

126

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Bone Tissue engineering (BTE) has recently been introduced as an alternative to conventional treatments for large non-healing bone defects. BTE approaches mimic autologous bone grafts, by combining cells, scaffold, and growth factors, and have the added benefit of being able to manipulate these constituents to optimize healing. Electrical stimulation (ES) has long been used to successfully treat non-healing fractures and has recently been shown to stimulate bone cells to migrate, proliferate, align, differentiate, and adhere to bio compatible scaffolds, all cell behaviors that could improve BTE treatment outcomes. With the above in mind we performed in vitro experiments and demonstrated that exposing Mesenchymal Stem Cells (MSC) + scaffold to ES for 3 weeks resulted in significant increases in osteogenic differentiation. Then in in vivo experiments, for the first time, we demonstrated that exposing BTE treated rat femur large defects to ES for 8 weeks, caused improved healing, as indicated by increased bone formation, strength, vessel density, and osteogenic gene expression. Our results demonstrate that ES significantly increases osteogenic differentiation in vitro and that this effect is translated into improved healing in vivo. These findings support the use of ES to help BTE treatments achieve their full therapeutic potential.

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Osteogenic differentiation of electrostimulated human mesenchymal stem cells seeded on silk-fibroin films

Cited by 2 publications

References 42 publications

Blood Stage Plasmodium falciparum Exhibits Biological Responses to Direct Current Electric Fields

Blood Stage Plasmodium falciparum Exhibits Biological Responses to Direct Current Electric Fields

Combining electrical stimulation and tissue engineering to treat large bone defects in a rat model

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