Osteogenic differentiation of human bone marrow-derived mesenchymal stem cells is enhanced by an aragonite scaffold

Matta, Csaba; Szűcs-Somogyi, Csilla; Kon, Elizaveta; Robinson, Dror; Neufeld, Tova; Altschuler, Nir; Berta, Ágnes; Hangody, Laszlo Rudolf; Veréb, Zoltán; Zákány, Róza

doi:10.1016/j.diff.2019.05.002

Cited by 56 publications

(37 citation statements)

References 46 publications

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“…Starting from day 14, the osteogenic-differentiating cells also began to express OCN, in agreement with the findings that OCN appears concomitantly with the mineralization phase of bone formation [72]. Also the increment of OPN expression over time well correlates with data available in the literature [53]. When hADSCs were exposed to HBO for all the duration of differentiation, a certain reduction in the expression profile of some of the above described markers was evidenced.…”

Section: Discussionsupporting

confidence: 90%

“…The reduced proliferation rate at later time points can also be regarded as a sign of progression in differentiation [53]. We therefore monitored the effects of the HBO treatment during hADSCs osteogenesis by measuring calcium deposition in the extracellular matrix, which is considered as one of the main parameters to assess osteogenic differentiation in vitro [54].…”

Section: Discussionmentioning

confidence: 99%

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Hyperbaric Oxygen Therapy Improves the Osteogenic and Vasculogenic Properties of Mesenchymal Stem Cells in the Presence of Inflammation In Vitro

Gardin

Bosco

Ferroni

et al. 2020

IJMS

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Hyperbaric oxygen (HBO) therapy has been reported to be beneficial for treating many conditions of inflammation-associated bone loss. The aim of this work was to in vitro investigate the effect of HBO in the course of osteogenesis of human Mesenchymal Stem Cells (MSCs) grown in a simulated pro-inflammatory environment. Cells were cultured with osteogenic differentiation factors in the presence or not of the pro-inflammatory cytokine Tumor Necrosis Factor-α (TNF-α), and simultaneously exposed daily for 60 min, and up to 21 days, at 2,4 atmosphere absolute (ATA) and 100% O2. To elucidate osteogenic differentiation-dependent effects, cells were additionally pre-committed prior to treatments. Cell metabolic activity was evaluated by means of the MTT assay and DNA content quantification, whereas osteogenic and vasculogenic differentiation was assessed by quantification of extracellular calcium deposition and gene expression analysis. Metabolic activity and osteogenic properties of cells did not differ between HBO, high pressure (HB) alone, or high oxygen (HO) alone and control if cells were pre-differentiated to the osteogenic lineage. In contrast, when treatments started contextually to the osteogenic differentiation of the cells, a significant reduction in cell metabolic activity first, and in mineral deposition at later time points, were observed in the HBO-treated group. Interestingly, TNF-α supplementation determined a significant improvement in the osteogenic capacity of cells subjected to HBO, which was not observed in TNF-α-treated cells exposed to HB or HO alone. This study suggests that exposure of osteogenic-differentiating MSCs to HBO under in vitro simulated inflammatory conditions enhances differentiation towards the osteogenic phenotype, providing evidence of the potential application of HBO in all those processes requiring bone regeneration.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Hyperbaric Oxygen Therapy Improves the Osteogenic and Vasculogenic Properties of Mesenchymal Stem Cells in the Presence of Inflammation In Vitro

Gardin

Bosco

Ferroni

et al. 2020

IJMS

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“…Our results indicate that both ALP and ColI proteins are highly expressed in 20% TCP‐CPC. The final stage is characterized by high expression of osteocalcin and osteopontin as well as the deposition of calcium and phosphate mineralized matrices (Matta et al, ). Mineralized nodule formation occurs during the last part of osteogenic induction.…”

Section: Discussionmentioning

confidence: 99%

Osteogenic stimulation of human dental pulp stem cells with self‐setting biphasic calcium phosphate cement

Zhuang

Xie

et al. 2019

J Biomed Mater Res

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Calcium phosphate cement (CPC) is widely used as a repair material for bone defects. However, there is no report on the use of biphasic CPC combined with dental pulp stem cells (DPSCs) for bone tissue engineering. The aim of this study was to construct biphasic CPC and to investigate its effect in the osteogenic stimulation of human DPSCs (hDPSCs) and macrophage polarization. Biphasic CPC was developed by adding different ratios of β‐TCP to an equimolar mixture of tetracalcium phosphate (TTCP) and dicalcium phosphate anhydrous (DCPA). The biocompatibility, osteogenic capacity, and inflammatory reactions of the cements were examined. Our results show that biphasic CPC containing both HA and β‐TCP was successfully fabricated. The addition of β‐TCP did not increase necrosis or apoptosis of DPSCs. Significant increases in hDPSC mineral deposition and osteogenic marker (ColI and ALP) expression were observed in 20% TCP‐CPC. The addition of β‐TCP did not promote macrophage polarization to a proinflammatory phenotype. In conclusion, biphasic CPC with hDPSCs for bone regeneration was developed for the first time; the biphasic CPC combined with hDPSCs is expected to be used for the repair and regeneration of bone.

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“…Promisingly, the cellular morphologies are comparable to interactions shown between osteogenic scaffolds and human BMSCs. 44 In contrast, BMSCs cells adhered to thermally treated scaffolds displayed less cell spreading, generally presenting a rounded globular morphology with fewer observable elongations (Fig. 6b).…”

Section: -Xca 2þmentioning

confidence: 95%

Chemobrionic structures in tissue engineering: self-assembling calcium phosphate tubes as cellular scaffolds

et al. 2020

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Osteogenic differentiation of human bone marrow-derived mesenchymal stem cells is enhanced by an aragonite scaffold

Cited by 56 publications

References 46 publications

Hyperbaric Oxygen Therapy Improves the Osteogenic and Vasculogenic Properties of Mesenchymal Stem Cells in the Presence of Inflammation In Vitro

Hyperbaric Oxygen Therapy Improves the Osteogenic and Vasculogenic Properties of Mesenchymal Stem Cells in the Presence of Inflammation In Vitro

Osteogenic stimulation of human dental pulp stem cells with self‐setting biphasic calcium phosphate cement

Chemobrionic structures in tissue engineering: self-assembling calcium phosphate tubes as cellular scaffolds

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